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Generation of Vibrio anguillarum Ghost by Coexpression of PhiX 174 Lysis E gene and Staphylococcal Nuclease A Gene

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Abstract

Vibrio anguillarum ghosts (VAG) were generated, for the first time, using a conjugation vector containing a ghost bacteria inducing cassette, pRK-λPR-cI-Elysis, in which the expression of PhiX174 lysis gene E was controlled by the P R /cI regulatory system of lambda phage. By scanning electron microscopy, holes ranging 80–200 nm in diameter were observed in the VAG. To avoid the presence of bacterial genomic DNA and an antibiotic resistance gene in the final VAG product, we constructed a new dual vector, pRK-λPR-cI-E-SNA, containing the E-mediated lysis cassette and the staphylococcal nuclease A (SNA)-mediated DNA degradation cassette, and generated safety-enhanced VAG for use as a fish vaccine.

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Acknowledgments

This study was supported by Korea Sea Grant Program (07-57Yeongnam001) from Ministry for Food, Agriculture, Forestry and Fisheries.

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Authors and Affiliations

  1. Department of Aquatic Life Medicine, Pukyong National University, Pusan, 608-737, Korea

    Se Ryun Kwon, Yue Jai Kang, Dong Jin Lee, Eun Hye Lee & Ki Hong Kim

  2. Department of Aquaculture, Pukyong National University, Pusan, 608-737, Korea

    Yoon Kwon Nam

  3. Faculty of Food Science and Biotechnology, Pukyong National University, Pusan, 608-737, Korea

    Sung Koo Kim

Authors
  1. Se Ryun Kwon
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  2. Yue Jai Kang
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  3. Dong Jin Lee
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  4. Eun Hye Lee
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  6. Sung Koo Kim
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  7. Ki Hong Kim
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Correspondence to Ki Hong Kim.

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Kwon, S.R., Kang, Y.J., Lee, D.J. et al. Generation of Vibrio anguillarum Ghost by Coexpression of PhiX 174 Lysis E gene and Staphylococcal Nuclease A Gene. Mol Biotechnol 42, 154–159 (2009). https://doi.org/10.1007/s12033-009-9147-y

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