Abstract
Cerebrovascular amyloidosis caused by amyloid accumulation in blood vessel walls may lead to hemorrhagic stroke and cognitive impairment. Expression of TGF-β1 under glial fibrillary acidic protein promoter in mice leads to age-related deposition of amyloid, including β-amyloid (Aβ), around cerebral blood vessels, leading to vascular pathology starting at age of 7 months. We have recently shown the important role of macrophages in clearing cerebrovascular amyloid. Scavenger receptor A (SRA) is a multi-ligand and multifunctional receptor expressed on macrophages, and it has been suggested to play a role in meditating phagocytosis of different types of antigens. We investigated the role of SRA in mediating cerebrovascular amyloid clearance. We bred TGF-β1 mice with SRA−/− mice and discovered that TGF-β1/SRA−/− mice showed cerebrovascular pathology at an earlier age (3 months) compared with TGF-β1 mice. Furthermore, SRA deficiency in macrophages led to impaired clearing of congophilic cerebrovascular amyloid from amyloid precursor protein mouse model and led to reduced phagocytosis of both soluble and insoluble Aβ in vivo as compared with macrophages from wild-type mice. Our findings demonstrate the important role of SRA in cerebrovascular amyloid pathology and suggest targeting SRA for future diagnostic and therapeutic approaches for cerebral amyloid angiopathy.
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Acknowledgments
We wish to thank Pro. Tony Wyss-Coray from Stanford University School of Medicine, USA, for providing TGF-β1 mice. This work is supported by grants from the Alzheimer's Association NIRG-11-205535 and ISF (to D.F.).
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Lifshitz, V., Weiss, R., Levy, H. et al. Scavenger Receptor A Deficiency Accelerates Cerebrovascular Amyloidosis in an Animal Model. J Mol Neurosci 50, 198–203 (2013). https://doi.org/10.1007/s12031-012-9909-z
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DOI: https://doi.org/10.1007/s12031-012-9909-z