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Genetic variations in interleukin-12B in allergic rhinitis

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Abstract

Interleukin (IL)-12B is a key regulator in the protection against allergic reaction induced by various allergens in patients with allergic rhinitis (AR). This study was performed to investigate the associations between single nucleotide polymorphisms in the IL-12B gene and AR in a Chinese Han population. A total of 543 patients with AR and 600 healthy controls were genotyped for IL-12B/rs6887695, rs10045431, rs3212227, rs3212217, and rs2082412 using polymerase chain reaction (PCR) restriction fragment length polymorphism and PCR direct sequencing. The expression of IL-12B mRNA in peripheral blood mononuclear cells (PBMCs) obtained from normal controls was detected by quantitative real-time PCR. Levels of interferon (IFN)-γ and IL-4 in culture supernatants were measured by enzyme-linked immunosorbent assay. The frequencies of the rs3212227 genotype with the CC and C alleles were higher in patients with AR (p c = 8.55 × 10−4; p c = 4.20 × 10−4, respectively) than in normal controls. After peptidoglycan stimulation in PBMCs, IL-12 mRNA expression was reduced in healthy carriers of the CC genotype of rs3212227. Additionally, decreased production of IFN-γ was observed in patients having the CC genotype as compared with that in patients having the AC and AA genotypes. In contrast, IL-4 expression was higher in patients with the CC genotype than in patients with the other two genotypes. The IL-12B gene was involved in mediating susceptibility to AR.

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Abbreviations

AR:

Allergic rhinitis

SNPs:

Single nucleotide polymorphisms

PCR–RFLP:

PCR restriction fragment length polymorphism assay

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Acknowledgments

The authors would like to thank very much for all the donors contributed to the present study.

Author Contributions

In this study, Ping Wei, Wei Kou, and Wu Wang conceived and designed the experiments. Ping Wei and Wei Kou performed the experiment in the laboratory. Houyong Kang, Xinye Tang, and Cheng Zhang collected the specimen. Ping Wei and Wei Kou analyzed the data and wrote the manuscript. Hongbing Yao and Wu Wang modified the thesis.

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Authors and Affiliations

  1. Department of Otolaryngology, The Children’s Hospital of Chongqing Medical University, Chongqing, 136# Zhongshaner Road, Chongqing, 400014, China

    Ping Wei, Wei Kou, Cheng Zhang, Hongbing Yao, Xinye Tang & Wu Wang

  2. Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China

    Ping Wei, Wei Kou, Cheng Zhang, Hongbing Yao, Xinye Tang & Wu Wang

  3. Key Laboratory of Pediatrics in Chongqing, Chongqing, China

    Ping Wei, Wei Kou, Cheng Zhang, Hongbing Yao, Xinye Tang & Wu Wang

  4. Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China

    Ping Wei, Wei Kou, Cheng Zhang, Hongbing Yao, Xinye Tang & Wu Wang

  5. Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

    Houyong Kang

Authors
  1. Ping Wei
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  2. Wei Kou
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  3. Cheng Zhang
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  4. Houyong Kang
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  5. Hongbing Yao
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  6. Xinye Tang
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  7. Wu Wang
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Corresponding author

Correspondence to Wu Wang.

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Conflict of interest

We would simply emphasize that after the discussion and decision of all authors, we recommend Ping Wei and Wei Kou as the co-first authors, as the equal contribution to this study. The authors declare that they have no competing interests.

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Cite this article

Wei, P., Kou, W., Zhang, C. et al. Genetic variations in interleukin-12B in allergic rhinitis. Immunol Res 64, 329–336 (2016). https://doi.org/10.1007/s12026-015-8758-6

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  • DOI: https://doi.org/10.1007/s12026-015-8758-6

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