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Expression of YY1 in Differentiated Thyroid Cancer

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Abstract

The transcription factor Yin Yang 1 (YY1) has an important regulatory role in tumorigenesis, but its implication in thyroid cancer has not been yet investigated. In the present study, we have analyzed the expression of YY1 in differentiated thyroid cancer and assessed the association of YY1 expression with clinical features. Expression of YY1 was evaluated in human thyroid cancer cell lines, a series of matched normal/tumor thyroid tissues and in a thyroid cancer tissue microarray, using real-time PCR, Western blot, and/or immunohistochemistry. YY1 was overexpressed in thyroid cancer cells, at transcription and protein levels. A significant increase of YY1 mRNA was also observed in tumor thyroid tissues. Moreover, immunohistochemical analysis of the thyroid cancer tissue microarray revealed that both papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC) present increased YY1 protein levels (48 and 19 %, respectively). After stratification by the level of YY1 protein, positive YY1 expression identifies 88 % of patients with PTC. The association of YY1 expression with clinicopathological features in PTC and FTC showed that YY1 expression was related with age at diagnosis. Our data indicates for the first time overexpression of YY1 in differentiated thyroid cancer, with YY1 being more frequently overexpressed in the PTC subtype.

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Acknowledgments

We thank all the subjects participating in this study and Cristian Valiente for his technical support. This work was partially funded by the Spanish Ministry of Education and Science (project SAF2007-6338) and the Generalitat de Catalunya (CIRIT; 2009SGR-725). J. Arribas was supported by a predoctoral fellowship (PIF) from the Universitat Autònoma de Barcelona.

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

Patient’s data were obtained and handled in accordance with the ethical standards of the Human Ethics Committee of the Hospital (Comitè Ètic d’Investigació Clínica, CEIC, Hospital Universitari Vall d’Hebron) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Authors and Affiliations

  1. Grup de Mutagènesi, Unitat de Genètica, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallés, Barcelona, Spain

    Jéssica Arribas, Ricard Marcos & Antonia Velázquez

  2. Department of Pathology, Hospital Universitari Vall d’Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain

    Josep Castellví

  3. CIBER Epidemiologia y Salud Pública, ISCIII, Madrid, Spain

    Ricard Marcos & Antonia Velázquez

  4. Diabetes and Metabolism Research Unit (VHIR) and Department of Endocrinology, Hospital Universitari Vall d’Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain

    Carles Zafón

  5. CIBER Diabetes y Enfermedades Metabólicas Asociadas, ISCIII, Madrid, Spain

    Carles Zafón

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  1. Jéssica Arribas
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  2. Josep Castellví
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  4. Carles Zafón
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  5. Antonia Velázquez
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Correspondence to Carles Zafón or Antonia Velázquez.

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Arribas, J., Castellví, J., Marcos, R. et al. Expression of YY1 in Differentiated Thyroid Cancer. Endocr Pathol 26, 111–118 (2015). https://doi.org/10.1007/s12022-015-9359-6

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  • DOI: https://doi.org/10.1007/s12022-015-9359-6

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