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Proteomic Identification of Syzygium cumini Seed Extracts by MALDI-TOF/MS

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Abstract

Syzygium cumini is traditionally used medicinal plant. The different part of the plant such as bark, leaves, seed and fruits are widely used as an alternative medicine in various diseases. Although the scientific community has a strong interest on S. cumini seed biochemistry focusing on metabolite composition, proteins have not yet been investigated. In the present study, we have applied a proteomic approach to study the proteome of the S. cumini seed using phenol extraction method for protein isolation, which were never analysed before. Fifteen brightly silver stained protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry after resolving on two-dimensional gel electrophoresis. These proteins have been found to involve in various functions such as antifungal, sulphur metabolism, carbohydrate metabolism, fruit ripening and softening, dormancy breaking and seed germination, hormone signalling, secondary metabolite transport, defence and stress response, nitrogen metabolism, synthesis and stabilization. Amongst the identified protein, lactoferrin was a mammalian origin protein with high nutritious and pharmaceutical value, which was purified by different types of chromatographic techniques and confirmed by western blotting. The antibacterial activity of lactoferrin was assessed by disc diffusion assay. We suggest that the protein constituents of S. cumini may have role in various functions required for plant physiology and its dietary values.

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Acknowledgments

This work was supported by Women Scientists Scheme (WOS-A), Department of Science and Technology, India. Kumari Binita thanks the Department of Science and Technology, India for her fellowship. Sanjay Kumar and Vinay Kumar Sharma also thank the Indian Council of Medical Research and All India Institute of Medical Sciences, New Delhi for their fellowships respectively.

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Authors and Affiliations

  1. Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India

    Kumari Binita, Sanjay Kumar, Vinay Kumar Sharma & Savita Yadav

  2. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India

    Veena Sharma

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  1. Kumari Binita
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  2. Sanjay Kumar
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  5. Savita Yadav
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Correspondence to Savita Yadav.

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Binita, K., Kumar, S., Sharma, V.K. et al. Proteomic Identification of Syzygium cumini Seed Extracts by MALDI-TOF/MS. Appl Biochem Biotechnol 172, 2091–2105 (2014). https://doi.org/10.1007/s12010-013-0660-x

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  • DOI: https://doi.org/10.1007/s12010-013-0660-x

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