Abstract
The study details the investigations on the ability of Lactobacillus plantarum CFR 2194, an isolate from kanjika, a rice-based ayurvedic fermented product, to produce biosurfactant. Surfactant production, as a function of fermentation time, indicates that the maximum production occurred at 72 h under stationary conditions. Isolation, partial purification, and characterization of the biosurfactant produced have been carried out, and Fourier transform infrared spectroscopy (FTIR) spectra demonstrated that biosurfactants were constituted by protein and polysaccharide fractions, i.e., possessed the structure typical of glycoprotein, which is affected by the medium composition and the phase of growth of the biosurfactant-synthesizing strain. Critical micelle concentration (cmc) of the biosurfactant was found to be 6 g l−1. The emulsification index (EI), emulsification activity (EA), and emulsion stability (ES) values of the biosurfactant have confirmed its emulsification property. Aqueous fractions of the produced biosurfactant exhibited a significant antimicrobial activity against the food-borne pathogenic species: Escherichia coli ATCC 31705, E. coli MTCC 108, Salmonella typhi, Yersinia enterocolitica MTCC 859, and Staphylococcus aureus F 722. More importantly, the biosurfactant from L. plantarum showed antiadhesive property against above food-borne pathogens. The results thus indicate the potential for developing strategies to prevent microbial colonization of food contact surfaces and health-care prosthesis using these biosurfactants.
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References
Singh, P., & Cameotra, S. S. (2004). Trends in Biotechnology, 22(3), 142–146.
Mohan, P. K., Nakhla, G., & Yanful, E. K. (2006). Water Research, 40, 533–540.
Flasz, A., Rocha, C. A., Mosquera, B., & Sajo, C. (1998). Medical Science Research, 26(3), 181–185.
Mulligan, C. N. (2005). Environmental Pollution, 133(2), 183–198.
Nitschke, M., & Costa, S. G. V. A. O. (2007). Trends in Food Science and Technology, 18, 252–259.
Banat, I. M., Makkar, R. S., & Cameotra, S. S. (2000). Applied Microbiology and Biotechnology, 53, 495–508.
Shepherd, R., Rockey, J., Sutherland, I. W., & Roller, S. (1995). Journal of Biotechnology, 40, 207–217.
Hood, S. K., & Zottola, E. A. (1995). Food Control, 6(1), 9–18.
Busscher, H. J., van der Kuij-Booij, M., & van der Mei, H. C. (1996). Journal of Industrial Microbiology and Biotechnology, 16(1), 15–21.
Makkar, R. S., & Cameotra, S. S. (2002). Applied Microbiology and Biotechnology, 58, 428–434.
Cameotra, S. S., & Makkar, R. S. (1998). Applied Microbiology and Biotechnology, 50, 520–529.
Makkar, R. S., & Cameotra, S. S. (1997). Journal of the American Oil Chemists Society, 74, 887–889.
Nitschke, M., & Pastore, G. M. (2003). Applied Biochemistry and Biotechnology, 105–108, 295–301.
Dubey, K., & Juwarkar, A. (2001). World Journal of Microbiology and Biotechnology, 17, 61–69.
Shete, A. M., Wadhawa, G., Banat, I. M., & Chopade, B. A. (2006). Journal of Science of Industrial Research, 65(2), 91–115.
Madhu, A. N., Giribhattanavar, P., Narayan, M. S., & Prapulla, S. G. (2010). Biotechnology Letters, 32, 503–506.
Madhu, A. N., & Prapulla, S. G. (2012). World Journal of Microbiology and Biotechnology, 28, 901–908.
Rodrigues, L. R., Teixeira, J. A., van der Mei, H. C., & Oliveira, R. (2006). Colloids and Surfaces B: Biointerfaces, 49, 78–85.
Cooper, D. G., & Goldenberg, B. G. (1987). Applied and Environmental Microbiology, 55(2), 224–227.
Calvo, C., Martinez, C. F., Mota, A., Bejar, V., & Quesada, E. (1998). Journal of Industrial Microbiology and Biotechnology, 20, 205–209.
Cirigliano, M. C., & Carman, G. M. (1985). Applied Environmental Microbiology, 50, 846–850.
Golek, P., Bednarski, W., Brzozowski, B., & Dziuba, B. (2009). Annals of Microbiology, 59(1), 119–126.
Reid, G., Heinemann, C., Velraeds, M. M. C., van der Mei, H. C., & Busscher, H. J. (1999). Methods in Enzymology, 310, 426–432.
Portilla, O. M., Moldes, A. B., Torrado, A. M., & Domínguez, J. M. (2008). Journal of Agriculture Food Chemistry, 56, 8074–8080.
Portilla, O. M., Rivas, B., Torrado, A., Moldes, A. B., & Domínguez, J. M. (2008). Journal of Science of Food and Agriculture, 88, 2298–2308.
Anukam, K. C., & Reid, G. (2007). Research Journal of Microbiology, 2(1), 81–87.
Bartosz, B., Wlodzimierz, B., & Bartlomiej, D. (2009). Journal of Science of Food and Agriculture, 89, 2467–2476.
Velraeds, M. M. C., van der Mei, H. C., Reid, G., & Busscher, H. J. (1996). Colloids and Surfaces B: Biointerfaces, 8, 51–61.
Busscher, H. J., van Hoogmoed, C. G., Geertsema-Doornbusch, G. I., van der Kuijl-Booij, M., & van der Mei, H. C. (1997). Applied Environmental Microbiology, 63, 3810–3817.
Lang, S., & Wullbrandt, D. (1999). Applied Microbiology and Biotechnology, 51, 22–32.
Schippers, C., Gebner, K., Muller, T., & Scheper, T. (2000). Journal of Biotechnology, 83, 189–198.
Moldes, A. B., Paradelo, R., Vecino, X., Cruz, J. M., Gudina, E., Rodrigues, L., Teixeira, J. A., Dominguez, J. M., & Barral, M. T. (2013). BioMed Research International. doi:10.1155/2013/961842. Article ID 961842, 6 pages.
Bello, X. V., Devesa-Rey, R., Cruz, J. M., & Moldes, A. B. (2012). Journal of Agriculture and Food Chemistry, 60, 1258–1265.
Rodrigues, L. R., Van der Mei, H. C., Teixeira, J. A., & Oliveira, R. (2004). Applied Environmental Microbiology, 70, 4408–4410.
Reid, G., Gan, B. S., Ens, W., Weinberger, S., & Howard, J. C. (2002). Applied Environmental Microbiology, 68, 977–980.
Rodrigues, L. R., Teixeira, J. A., Van der Mei, H. C., & Oliveira, R. (2006). Colloids and Surfaces B: Biointerfaces, 53, 105–112.
Rodrigues, L. R., Banat, I. M., van der Mei, H. C., Teixeira, J. A., & Oliveira, R. (2006). Journal of Applied Microbiology, 100(3), 470–480.
Falagas, M. E., & Makris, G. C. (2009). Journal of Hospital Infection, 71, 301–306.
Acknowledgments
A.N. Madhu is thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the award of Senior Research Fellowship. Esther Magdalene Sharon, a project student, was kindly acknowledged for help in carrying out the experiments. Dr. Purnima Kaul Tiku, a scientist, of the Protein Chemistry and Nutrition, CFTRI, is greatly acknowledged for the help in using the tensiometer. The authors thank the Director, CFTRI, for supporting the work.
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Madhu, A.N., Prapulla, S.G. Evaluation and Functional Characterization of a Biosurfactant Produced by Lactobacillus plantarum CFR 2194. Appl Biochem Biotechnol 172, 1777–1789 (2014). https://doi.org/10.1007/s12010-013-0649-5
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DOI: https://doi.org/10.1007/s12010-013-0649-5