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Immobilization of Candida antarctica lipase onto cellulose acetate-coated Fe2O3 nanoparticles for glycerolysis of olive oil

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Abstract

Candida antarctica lipase was covalently immobilized onto the surface of cellulose acetate-coated Fe2O3 nanoparticles. The characterizations of immobilized lipase were examined by Fourier transform infrared spectrophotometer (FTIR) and field emission gun-scanning electron microscopy (FEG-SEM). The immobilized lipase was assayed for production of monoglycerides (MG) and diglycerides (DG) by glycerolysis of olive oil in a solvent medium. The effect of various reaction conditions on the MG and DG production such as reaction time, temperature, the molar ratio of glycerol to oil and amount of immobilized lipase was investigated. The optimum condition for MG and DG production was found at 50 °C temperature and 0.025 g of lipase with the molar ratio of glycerol to oil 1.5: 1 in 5 h of reaction time. The effect of substrate concentration on enzymatic activity of the free and immobilized lipase showed the best fits to the Lineweaver-Burk plots. The K m and V max values of immobilized lipase were found to be 25mM and 0.58mM/min, whereas that for free lipase was 52.63mM and 1.75mM/min, respectively. The activation and deactivation energy was found to decrease for immobilization of lipase on cellulose acetate-coated Fe2O3 nanoparticles.

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Refrerences

  1. A. Chaubey, R. Parshad, S. C. Taneja and G. N. Qazi, Process Biochem., 44, 154 (2009).

    Article  CAS  Google Scholar 

  2. H. Zeng, K. Liao, X. Deng, H. Jiang and F. Zhang, Process Biochem., 44, 791 (2009).

    Article  CAS  Google Scholar 

  3. M. Karra-Châabouni, I. Bouaziz, S. Bou, A. M.B. Rego and Y. Gargouri, Colloids Surf. B, 66, 168 (2008).

    Article  Google Scholar 

  4. C. Pizarro, M. Fernandez-Torroba, C. Benito and J. Gonzalez-Saiz, Biotechnol. Bioeng., 53, 497 (1997).

    Article  CAS  Google Scholar 

  5. M. Kamori, T. Hori, M. Yamashita, T. Hori, Y. Yamashita, Y. Hirose and N. Naoshinobu, J. Mol. Catal. B: Enzym., 9, 269 (2000).

    Article  CAS  Google Scholar 

  6. R. Dalla-Vecchia, D. Sebrao, M.G. Nascimento and V. Soldi, Process Biochem., 40, 2677 (2005).

    Article  CAS  Google Scholar 

  7. A. K. Singh and M. Mukhopadhyay, Appl. Biochem. Biotechnol., 166, 486 (2012).

    Article  CAS  Google Scholar 

  8. M. H. Kim, S. An, K. Won, H. J. Kim and S. H. Lee, J. Mol. Catal. B: Enzym., 75, 68 (2012).

    Article  CAS  Google Scholar 

  9. S. S. Betigeri and S. H. Neau, Biomater., 23, 3627 (2002).

    Article  CAS  Google Scholar 

  10. A. B. Majumder and M. N. Gupta, Bioresour. Technol., 101, 2877 (2010).

    Article  CAS  Google Scholar 

  11. Y. Yesiloglu, Process Biochem., 40, 2155 (2005).

    Article  CAS  Google Scholar 

  12. J. Brem, M.C. Turcu, C. Paizs, K. Lundell, M. Tosa, F. Irimie and L. T. Kanerva, Process Biochem., 47, 119 (2012).

    Article  CAS  Google Scholar 

  13. S. Sabbani, E. Hedenstrom and O. Nordin, J. Mol. Catal. B: Enzym., 42, 1 (2006).

    Article  CAS  Google Scholar 

  14. H. Ghamgui, N. Miled, M. Karra-chaabouni and Y. Gargouri, Biochem. Eng. J., 37, 34 (2007).

    Article  CAS  Google Scholar 

  15. E. Yilmaz, K. Can, M. Sezgin and M. Yilmaz, Bioresour. Technol., 102, 499 (2011).

    Article  CAS  Google Scholar 

  16. M. Zheng, S. Zhang, G. Ma and P. Wang, J. Biotechnol., 154, 274 (2011).

    Article  CAS  Google Scholar 

  17. K. M. Ponvel, D.G. Lee, E. J. Woo, I. S. Ahn and C. H. Lee, Korean J. Chem. Eng., 26, 127 (2009).

    Article  CAS  Google Scholar 

  18. E. Serra, E. Díez, I. Díaz and R.M. Blanco, Micropor. Mesopor. Mater., 132, 487 (2010).

    Article  CAS  Google Scholar 

  19. M. Guncheva, M. Dimitrov and D. Zhiryakova, Process Biochem., 46, 2170 (2011).

    Article  CAS  Google Scholar 

  20. Y. Z. Chen, C. B. Ching and R. Xu, Process Biochem., 44, 1245 (2009).

    Article  CAS  Google Scholar 

  21. Y. Wu, Y. Wang, G. Luo and Y. Dai, Bioresour. Technol., 101, 841 (2010).

    Article  CAS  Google Scholar 

  22. P. R. Solanki, C. Dhand, A. Kaushik, A. A. Ansari, K. N. Sood and B. D. Malhotra, Sens. Actuators, B, 141, 551 (2009).

    Article  CAS  Google Scholar 

  23. E. Ranjbakhsh, A. K. Bordbar, M. Abbasi, A. R. Khosropour and E. Shams, Chem. Eng. J., 179, 272 (2012).

    Article  CAS  Google Scholar 

  24. X. Liu, L. Lei, Y. Li, H. Zhu, Y. Cui and H. Hu, Biochem. Eng. J., 56, 142 (2011).

    Article  CAS  Google Scholar 

  25. M. Namdeo and S. K. Bajpai, J. Mol. Catal. B: Enzym., 59, 134 (2009).

    Article  CAS  Google Scholar 

  26. S. Rauf, A. Ihsan, K. Akhtar, M. A. Ghauri, M. Rahman, M. A. Anwar and A. M. Khalid, J. Biotechnol., 121, 351 (2006).

    Article  CAS  Google Scholar 

  27. Y. Wu, Y. J. Wang, G. S. Luo and Y. Y. Dai, Bioresour. Technol., 100, 3459 (2008).

    Article  Google Scholar 

  28. D.G. Lee, K.M. Ponvel, M. Kim, S. Hwang, I. S. Ahn and C. H. Lee, J. Mol. Catal. B: Enzym., 57, 62 (2009).

    Article  CAS  Google Scholar 

  29. Y. Yong, Y.X. Bai, Y. F. Li, L. Lin, Y. J. Cui and C.G. Xia, J. Magn. Magn. Mater., 320, 2350 (2008).

    Article  CAS  Google Scholar 

  30. B. Hu, J. Pan, H. L. Yu, J.W. Liu and J. H. Xu, Process Biochem., 44, 1019 (2009).

    Article  CAS  Google Scholar 

  31. B. Chen, J. Hu, E. M. Miller, W. Xie, M. Cai and R. A. Gross, Biomacromolecules, 9, 463 (2008).

    Article  CAS  Google Scholar 

  32. C. González, J.M. Resa and J. Lanz, J. Am. Oil Chem. Soc., 77, 985 (2000).

    Article  Google Scholar 

  33. O. H. Lowry, M. J. Rosenbrough, A. L. Farr and R. J. Randell, J. Biol. Chem., 193, 265 (1951).

    CAS  Google Scholar 

  34. B. Stellmach, Bestimmungsmethoden enzyme, Chinese Light Industry Press, Chinese (1992).

    Google Scholar 

  35. A. Idris, N.A.M. Zain and M. S. Suhaimi, Process Biochem., 43, 331 (2008).

    Article  CAS  Google Scholar 

  36. F. J. Kao, S. A. Ekhorutomwen and S. P. Sawan, Biotechnol. Technol., 11, 849 (1997).

    Article  CAS  Google Scholar 

  37. M.Y. Changa and R. S. Juangb, Enzyme Microb. Technol., 36, 75 (2005).

    Article  Google Scholar 

  38. Q. Z. K. Zhou and X. D. Chen, Biochem. Eng. J., 9, 33 (2001).

    Article  CAS  Google Scholar 

  39. C. A. Borgo, A.M. Lazarin, Y.V. Kholin, R. Landers and Y. Gushikem, J. Braz. Chem. Soc., 15, 50 (2004).

    Article  CAS  Google Scholar 

  40. M. L. Foresti and M. L. Ferreira, Catal. Today, 107, 23 (2005).

    Article  Google Scholar 

  41. M. L. Damstrup, T. Jensen, F.V. Sparsø, S. Z. Kiil, A. D. Jensen and X. Xu, J. Am. Oil Chem. Soc., 8, 559 (2005).

    Article  Google Scholar 

  42. A.K. Singh and M. Mukhopadhyay, Grasas Aceites, 63, 202 (2012).

    Article  CAS  Google Scholar 

  43. N. Zhong, L. Li, X. Xu, L. Cheong, B. Li, S. Hu and X. Zhao, J. Am. Oil Chem. Soc., 86, 783 (2009).

    Article  CAS  Google Scholar 

  44. A. Valério, R. L. Kruger, J. Ninow, F. C. Corazza, D. D. Oliveira, J.V. Oliveira and M. L. Corazza, J. Agric. Food Chem., 57, 8350 (2009).

    Article  Google Scholar 

  45. G. P. McNeill and T. Yamane, J. Am. Oil Chem. Soc., 68, 6 (1991).

    Article  CAS  Google Scholar 

  46. D. E. Stevenson, R.A. Stanley and R.H. Furneaux, Biotechnol. Lett., 15, 1043 (1993).

    Article  CAS  Google Scholar 

  47. M. Tüter and H. A. Aksoy, Biotechnol. Lett., 22, 31 (2000).

    Article  Google Scholar 

  48. T. Yamane, S. T. Kang, K. Kawahara and Y. Koizumi, J. Am. Oil Chem. Soc., 71, 339 (1994).

    Article  CAS  Google Scholar 

  49. C. Brady, L. Metcalfe, D. Slaboszewski and D. Frank, J. Am. Oil Chem. Soc., 65, 917 (1988).

    Article  CAS  Google Scholar 

  50. D. Yang and J. S. Rhee, Biotechnol. Lett., 13, 553 (1991).

    Article  CAS  Google Scholar 

  51. R. Pawongrat, X. Xu and A. H-Kittikun, Food Chem., 104, 251 (2007).

    Article  CAS  Google Scholar 

  52. F. N. Xi, J.M. Wu, Z. S. Jia and X. F. Lin, Process Biochem., 40, 2833 (2005).

    Article  CAS  Google Scholar 

  53. F. M. Gomes, E.B. Pereira and F. H. Decastro, Biomacromolecules, 5, 17 (2004).

    Article  CAS  Google Scholar 

  54. H. Tümtürk, N. Karaca, G. Demirel and F. ahin, Int. J. Biol. Macromol., 40, 281 (2007).

    Article  Google Scholar 

  55. Y. Yang, Y. X. Bai, Y. F. Li, L. Lei, Y. J. Cui and C.G. Xia, Process Biochem., 43, 1179 (2008).

    Article  Google Scholar 

  56. H. Kim, H. S. Kwon, J. Ahn, C. H. Lee and I. S. Ahn, Biocatal. Biotransform., 27, 246 (2009).

    Article  CAS  Google Scholar 

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  1. Department of Chemical Engineering, Sardar Vallabhbhai National Institute of Technology, Surat, 395007, Gujarat, India

    Abhishek Kumar Singh & Mausumi Mukhopadhyay

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  1. Abhishek Kumar Singh
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  2. Mausumi Mukhopadhyay
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Correspondence to Mausumi Mukhopadhyay.

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Singh, A.K., Mukhopadhyay, M. Immobilization of Candida antarctica lipase onto cellulose acetate-coated Fe2O3 nanoparticles for glycerolysis of olive oil. Korean J. Chem. Eng. 31, 1225–1232 (2014). https://doi.org/10.1007/s11814-014-0020-8

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