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Measurements of freezing tolerance and their relationship with some biochemical and physiological parameters in seven olive cultivars

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Abstract

The freezing tolerance of seven olive cultivars, including Amphisis, Conservallia, Koroneiki, Manzanilla, Gorgan, Shengeh, and Rashid, was compared, and its correlation with some biochemical and physiological factors was investigated. From each cultivar, leaf and stem samples were collected and sorted into two groups: the first was exposed to various freezing temperatures to indicate its freezing tolerance in terms of lethal temperature (LT50) values by several approaches (electrolyte leakage, maximum quantum yield of PSII photochemistry, and tetrazolium staining test); the second was prepared for some biochemical and physiological parameter analyses. Of all cultivars, Amphisis and Shengeh showed the highest freezing tolerance, the maximum soluble carbohydrate and proline content, DPPH (1, 1-diphenyl–2-picrylhydrazyl) scavenging capacity, the minimum malondialdehyde (MDA), and relative water content (RWC). Conservallia, Gorgan, and Manzanilla cultivars could moderately tolerate freezing temperatures, whereas Rashid and Koroneiki cultivars turned out to be freezing-susceptible. In olive bark and leaf, LT50 showed a negative correlation with soluble carbohydrate, proline content, and DPPH scavenging capacity and a positive correlation with MDA and RWC. Three indicators, i.e., LT50, osmoregulant content, and DPPH scavenging capacity, can contribute to screening the freezing-tolerant olive cultivars.

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References

  • Adams GT, Perkins TD (1993) Assessing cold tolerance in Picea using chlorophyll fluorescence. Environ exper bot 33:377–382

    Article  CAS  Google Scholar 

  • Aparicio R, Luna G (2002) Characterisation of monovarietal virgin olive oils. Eur J Lipid Sci Technol 104:614–627

    Article  CAS  Google Scholar 

  • Arias NS, Bucci SJ, Scholz FG, Goldstein G (2015) Freezing avoidance by supercooling in Olea europaea cultivars: the role of apoplastic water solute content and cell wall rigidity. Plant Cell Environ 38:2061–2070

    Article  CAS  Google Scholar 

  • Arora R, Wisniewski ME, Scorza R (1992) Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica [L.] Batsch). Plant Physiol 99:1562–1568

    Article  CAS  Google Scholar 

  • Azzarello E, Mugnai S, Pandolfi C, Masi E, Marone E, Mancuso S (2009) Comparing image (fractal analysis) and electrochemical (impedance spectroscopy and electrolyte leakage) techniques for the assessment of the freezing tolerance in olive. Trees 23:159–167

    Article  Google Scholar 

  • Ball MC, Canny MJ, Huang CX, Heady RD (2004) Structural changes in acclimated and unacclimated leaves during freezing and thawing. Funct Plant Biol 31:29–40

    Article  Google Scholar 

  • Barranco D, Ruiz N, Gomez-del-Campo M (2005) Frost tolerance of eight olive cultivars. HortScience 40:558–560

    Google Scholar 

  • Bartolozzi F, Fontanazza G (1999) Assessment of frost tolerance in olive (Olea europaea L.). Sci Hortic 81:309–319

    Article  Google Scholar 

  • Bartolozzi F, Mencuccini M, Fontanazza G (2001) Enhancement of frost tolerance in olive shoots in vitro by cold acclimation and sucrose increase in the culture medium. Plant Cell Tissue Organ Cult 67:299–302

    Article  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Cai Q, Wang S, Cui Z, Sun J, Ishii Y (2004) Changes in freezing tolerance and its relationship with the contents of carbohydrates and proline in overwintering centipedegrass (Eremochloa ophiuroides (Munro) Hack.). Plant prod sci 7:421–426

    Article  CAS  Google Scholar 

  • Cansev A, Gulen H, Eris A (2009) Cold-hardiness of olive (Olea europaea L.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins. J Agric Sci 147:51–61

    Article  CAS  Google Scholar 

  • Cheung LM, Cheung PCK, Ooi VEC (2003) Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 81:249–255

    Article  CAS  Google Scholar 

  • Clement JMAM, Van Hasselt PR (1996) Chlorophyll fluorescence as a parameter for frost hardiness in winter wheat. A comparison with other hardiness parameters. Phyton Ann Rei Bot Austria 36:29–41

    CAS  Google Scholar 

  • Couée I, Sulmon C, Gouesbet G, ElAmrani A (2006) Involvement of solubles ugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57:449–459

    Article  Google Scholar 

  • Danyluk J, Perron A, Houde M, Limin A, Fowler B, Benhamou N, Sarhan F (1998) Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat. Plant Cell 10:623–638

    Article  CAS  Google Scholar 

  • Dionne J, Castonguay Y, Nadeau P, Desjardins Y (2001) Freezing tolerance and carbohydrate changes during cold acclimation of green–type annual bluegrass (Poa annua L.) ecotypes. Crop Sci 41:443–451

    Article  CAS  Google Scholar 

  • Eris A, Gulen H, Barut E, Cansev A (2007) Annual patterns of total soluble sugars and proteins related to cold–hardiness in olive (Olea europaea L. ‘Gemlik’). J Hort Sci Biotechnol 82:597–604

    Article  CAS  Google Scholar 

  • Ershadi A, Karimi R, Naderi Mahdei K (2016) Freezing tolerance and its relationship with soluble carbohydrates proline and water content in 12 grapevine cultivars. Acta Physiol Plant 38:2–10

    Article  Google Scholar 

  • Fiorino P, Mancuso S (2000) Differential thermal analysis supercooling and cell viability in organs of Olea europaea at subzero temperatures. Adv Hort Sci 14:23–27

    Google Scholar 

  • Ghasemi Soloklui AA, Ershadi A, Fallahi E (2012) Evaluation of cold hardiness in seven Iranian commercial pomegranates (Punica granatum L.) cultivars. HortScience 47:1821–1825

    Article  Google Scholar 

  • Gilmore AM, Matsubara S, Ball MC, Barker DH, Itoh S (2003) Excitation energy flow at 77 K in the photosynthetic apparatus of overwintering evergreens. Plant Cell Environ 26:1021–1034

    Article  Google Scholar 

  • Gomez–del–Campo M, Barranco D (2005) Field evaluation of frost tolerance in 10 olive cultivars. Plant Genet Resour 3:385–390

    Article  Google Scholar 

  • Guerra D, Lamontanara A, Bagnaresi P, Orrù L, Rizza F, Zelasco S, Beghè D, Ganino T, Pagani D, Cattivelli L, Mazzucotelli E (2015) Transcriptome changes associated with cold acclimation in leaves of olive tree (Olea europaea L.). Tree Genet Genomes 11:113

    Article  Google Scholar 

  • Gulcin I, Alici HA, Cesur M (2005) Determination of in vitro antioxidant and radical scavenging activities of propofol. Chem Pharm Bull 53:281–285

    Article  Google Scholar 

  • Gulen H, Cansev A, Eris A (2009) Cold hardiness of olive (Olea europaea L.) cultivars in cold-acclimated and non-acclimated stages: seasonal alteration of soluble sugars and phospholipids. J Agric Sci 147:459–467

    Article  CAS  Google Scholar 

  • Gusta LV, Wisniewski M (2013) Understanding plant cold hardiness: an opinion. Physiol Plan 147:4–14

    Article  CAS  Google Scholar 

  • Gusta LV, Wisniewski M, Nesbitt NT, Gusta ML (2004) The effect of water, sugars, and proteins on the pattern of ice nucleation and propagation in acclimated and non-acclimated canola leaves. Plant Physiol 135:1642–1653

    Article  CAS  Google Scholar 

  • Guy CL (1990) Cold acclimation and freezing stress tolerance: role of protein metabolism. Ann Rev Plant Physiol Plant Mol Biol 41:187–223

    Article  CAS  Google Scholar 

  • Hashempour A, Ghasemnezhad M, FotouhiGhazvini R, Sohani MM (2014a) Olive (Olea europaea L.) freezing tolerance related to antioxidant enzymes activity during cold acclimation and non acclimation. Acta Physiol Plant 36:3231–3241

    Article  CAS  Google Scholar 

  • Hashempour A, Ghasemnezhad M, Ghazvini RF, Sohani MM (2014b) The physiological and biochemical responses to freezing stress of olive plants treated with salicylic acid. Russ J plant physiol 61:443–450

    Article  CAS  Google Scholar 

  • Hatfield JL, Prueger JH (2015) Temperature extremes: effect on plant growth and development. Weather Clim Extrem 10:4–10

    Article  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts I Kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  Google Scholar 

  • Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

    Article  CAS  Google Scholar 

  • Hou YD, Guo ZF, Yi Y, Li HN, Li HG, Chen LJ, Ma H, Zhang L, Lin JW, Zhong M (2010) Effects of cold acclimation and exogenous pytohormone abscisic acid treatment on physiological indicators of winterness wheat. J Plant Sci 5:125–136

    Article  CAS  Google Scholar 

  • Irigoyen JJ, Emerich DW, Sanchez–Diaz M (1992) Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60

    Article  CAS  Google Scholar 

  • Jacobsen SE, Monteros C, Christiansen JL, Bravo LA, Corcuera LJ, Mujica A (2005) Plant responses of quinoa (Chenopodium quinoa Willd.) to frost at various phonological stages. Eur J Agron 22:131–139

    Article  Google Scholar 

  • Kang HM, Saltveit ME (2002) Chilling tolerance of maize cucumber and rice seedling leaves and roots are differentially affected by salicylic acid. Physiol Plant 115:571–576

    Article  CAS  Google Scholar 

  • Krause GH (1988) Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms. Physiol Plant 74:566–574

    Article  CAS  Google Scholar 

  • Li J, Arkorful E, Cheng S, Zhou Q, Li H, Chen X, Sun K, Li X (2018) Alleviation of cold damage by exogenous application of melatonin in vegetatively propagated tea plant (Camellia sinensis (L.) O. Kuntze). Sci Hortic 238:356–362

    Article  CAS  Google Scholar 

  • Mancuso S (2000) Electrical resistance changes during exposure to low temperature measure chilling and freezing tolerance in olive tree (Olea europaea L.) plants. Plant Cell Environ 23:291–299

    Article  Google Scholar 

  • Mills LJ, Ferguson JC, Keller M (2006) Cold-hardiness evaluation of grapevine buds and cane tissues. Am J Enol Vitic 57:194–200

    Google Scholar 

  • Nesbitt ML, Ebel RC, Findley D, Wilkins B, Woods F, Himelrick D (2002) Assays to assess freeze injury of Satsuma mandarin. HortScience 37:871–877

    Article  Google Scholar 

  • Nishizawa A, Yabuta Y, Shigeoka S (2008) Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Plant Physiol 147:1251–1263

    Article  CAS  Google Scholar 

  • Ortega–Garcı´a F, Perago´n J (2009) The response of phenylalanine ammonia–lyase polyphenol oxidase and phenols to cold stress in the olive tree (Olea europaea L. cv Picual). J Sci Food 89:1565–1573

    Article  Google Scholar 

  • Palliotti A, Bongi G (1996) Freezing injury in the olive leaf and effects of mefluidide treatment. Sci Hortic 71:57–63

    Article  CAS  Google Scholar 

  • Palta JP, Levitt J, Stadelman EJ (1977) Freezing injury in onion bulbs cells II post-thawing injury or recovery. Plant Physiol 60:398–401

    Article  CAS  Google Scholar 

  • Proietti P, Famiani F (2002) Diurnal and seasonal changes in photosynthetic characteristics in different olive (Olea europaea L.) cultivars. Photosynthetica 40:171–176

    Article  CAS  Google Scholar 

  • Rihan HZ, Al–Issawi M, Fuller MP (2017) Advances in physiological and molecular aspects of plant cold tolerance. J Plant Interact 12:143–157

    Article  CAS  Google Scholar 

  • Roselli G, Venora G (1990) Relationship between stomatal size and winter hardiness in the olive. Acta Hort 286:89–92

    Article  Google Scholar 

  • Roselli G, Benelli G, Morelli D (1989) Relationship between stomatal density and winter hardiness in olive (Olea europaea L.). HortScience 64:199–203

    Google Scholar 

  • Roselli G, La Porta N, Morelli D (1992) Valutazioni del germoplasma di olivo per la tolleranza a stress da freddo. Anti Convegno Germoplasma Frutticolo 9:107–112

    Google Scholar 

  • Rugini E, Biasi R, Muleo R (2000) Olive (Olea europaea var sativa) transformation. In: Jain SM, Minocha SC (eds) Molecular biology of woody plants. Springer, Dordrecht, pp 245–279

    Google Scholar 

  • Sarikhani H, Haghi H, Ershadi A, Esna–Ashari M, Pouya M (2014) Foliar application of potassium sulphate enhances the cold–hardiness of grapevine (Vitis vinifera L.). J Hort Sci Biotechnol 89:141–146

    Article  CAS  Google Scholar 

  • Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species oxidative damage and antioxidative defense mechanism in plants under stressful conditions. J Bot 2012:1–26

    Article  Google Scholar 

  • Simkeshzadeh N, Mobli M, Etemadi N, Baninasab B (2011) Assessment of the frost resistance in some olive cultivars using visual injuries and chlorophyll fluorescence. J Hort Sci 4:163–169 (In Persian with English abstract)

    Google Scholar 

  • Solecka D, Kacperska A (2003) Phenylpropanoid deficiency affects the course of plant acclimation to cold. Physiol Plant 119:253–262

    Article  CAS  Google Scholar 

  • Steponkus PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Ann Rev Plant Physiol 35:543–584

    Article  CAS  Google Scholar 

  • Taylor CB (1996) Proline and water deficit: ups and downs. Plant Cell 8:1221–1224

    Article  CAS  Google Scholar 

  • Wang M, Li J, Rangarajan M, Shao Y, LaVoie EJ, Huang T, Ho Ch (1998) Antioxidative phenolic compounds from sage (Salvia officinalis). J Agric Food Chem 46:4869–4873

    Article  CAS  Google Scholar 

  • Weiser CJ (1970) Cold resistance and injury in woody plants: knowledge of hardy plant adaptations to freezing stress may help us to reduce winter damage. Science 169:1269–1278

    Article  CAS  Google Scholar 

  • Wise RR (1995) Chilling–enhanced photooxidation: the production action and study of reactive oxygen species produced during chilling in the light. Photosynth Res 45:79–97

    Article  CAS  Google Scholar 

  • Xin Z, Li PH (1993) Relationship between proline and abscisic acid in the induction of chilling tolerance in maize suspension-cultured cells. Plant Physiol 103:607–613

    Article  CAS  Google Scholar 

  • Zhang X, Ervin EH, LaBranche AJ (2006) Metabolic defense responses of seeded bermudagrass during acclimation to freezing stress. Crop Sci 46:2598–2605

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to express their sincere gratitude to Dr. M. R. Sabzalian and Mr. R. Mohammadi for their valuable help with this experiment. This study was supported by the Research Council of Isfahan University of Technology.

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Authors and Affiliations

  1. Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Safoora Saadati, Bahram Baninasab, Mostafa Mobli & Mahdiyeh Gholami

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  1. Safoora Saadati
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  2. Bahram Baninasab
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  3. Mostafa Mobli
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  4. Mahdiyeh Gholami
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Correspondence to Bahram Baninasab.

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Communicated by L. Bavaresco.

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Saadati, S., Baninasab, B., Mobli, M. et al. Measurements of freezing tolerance and their relationship with some biochemical and physiological parameters in seven olive cultivars. Acta Physiol Plant 41, 51 (2019). https://doi.org/10.1007/s11738-019-2843-8

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  • DOI: https://doi.org/10.1007/s11738-019-2843-8

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