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Comparative proteomic analysis of cold-induced sweetening in potato (Solanum tuberosum L.) tuber

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Abstract

Cold-induced sweetening is one of the major factors limiting the quality of fried potato products. To understand the mechanisms of protein regulation for cold-induced sweetening in potato tubers, a comparative proteomic approach was used to analyse the differentially expressed proteins both during control (25 °C, 30 days) and cold treatment (4 °C, 30 days) using two-dimensional gel electrophoresis. Quantitative image analyses indicated that there were 25 protein spots with their intensities significantly altered more than twofold. Of these proteins, 9 were up-regulated, 13 were down-regulated, 2 were absent, and 1 was induced in the cold-stored tubers. The MALDI-TOF/TOF MS analyses led to the identification of differentially expressed proteins that are involved in several processes and might work cooperatively to maintain metabolic homeostasis in tubers during low-temperature storage. The preponderance of metabolic proteins reflects the inhibition of starch re-synthesis and the accumulation of sugars in carbon fluxes, linking starch–sugar conversion. The respiration-related proteins suggest the transfer of respiratory activity from aerobic respiration to anaerobic respiration in the cold-stored tubers. The proteins associated with defence appear to protect the tuber cells from low-temperature stress. Some heat shock proteins that act as chaperones also displayed a differential expression pattern, suggesting a potentially important role in cold-stored tubers, although their exact contribution remains to be investigated. The proposed hypothetical model might explain the interaction of these differentially expressed proteins that are associated with cold-induced sweetening in tubers.

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Acknowledgments

This work was supported by program for National Natural Science Foundation (30960205, 31171477), New Century Excellent Talents in University (NCET-07-0214), Innovative Basic Research Groups of Gansu (1308RJIA005), International S&T Cooperation Program of China (2014DFG31570), Gansu Provincial Key Laboratory of Aridland Crop Science (GSCS-02), Gansu Educational Science Foundation (1002-07), Sheng Tongsheng Science Technology and Innovation Foundation of Gansu Agricultural University (GSAU-STS-1223).

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  1. Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Gansu Provincial Key Laboratory of Aridland Crop Science, Research and Testing Center, Gansu Agricultural University, Lanzhou, 730070, China

    Lixiang Cheng, Yuping Wang, Dongxia Wang, Di Wang & Feng Zhang

  2. College of Life Science and Technology, Gansu Agricultural University, Lanzhou, China

    Xin Zhang, Qingxia Zhao, Huijun Li & Feng Zhang

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  1. Lixiang Cheng
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  2. Xin Zhang
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  3. Qingxia Zhao
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  5. Yuping Wang
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  7. Di Wang
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Correspondence to Di Wang or Feng Zhang.

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Communicated by M. Stobiecki.

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Cheng, L., Zhang, X., Zhao, Q. et al. Comparative proteomic analysis of cold-induced sweetening in potato (Solanum tuberosum L.) tuber. Acta Physiol Plant 36, 1197–1210 (2014). https://doi.org/10.1007/s11738-014-1496-x

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