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Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.) G. Don]

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Abstract

A pot experiment was conducted to find out whether the foliar spray of salicylic acid (SA) could successfully ameliorate the adverse effects of salinity stress on periwinkle. Thirty-day-old plants were supplied with Control; 0 mM NaCl + 10−5 M SA (T1); 50 mM NaCl + 0 SA (T2); 100 mM NaCl + 0 SA (T3); 150 mM NaCl + 0 SA (T4); 50 mM NaCl + 10−5 M SA (T5); 100 mM NaCl + 10−5 M SA (T6); 150 mM NaCl + 10−5 M SA (T7). The plants were sampled 90 days after sowing to assess the effect of SA on stressed and unstressed plants. Salt stress significantly reduced the growth attributes including plant height, leaf-area index, shoot and root fresh weights, shoot and root dry weights. Increasing NaCl concentrations led to a gradual decrease in photosynthetic parameters and activities of nitrate reductase and carbonic anhydrase. Ascorbic acid, total alkaloids and antioxidants enzymes superoxide dismutase, catalase and peroxidase also declined in NaCl-treated plants. The plants, undergoing NaCl stress, exhibited a significant increase in electrolyte leakage and proline content. Foliar application of SA (10−5 M) reduced the damaging effect of salinity on plant growth and accelerated the restoration of growth processes. It not only improved the growth parameters but also reversed the effects of salinity. Total alkaloid content was improved by SA application both in unstressed and stressed plants. The highest level of total alkaloid content recorded in leaves of SA-treated stressed plants was 11.1%. Foliar spray of SA overcame the adverse effect of salinity by improving the content of vincristine (14.0%) and vinblastine (14.6%) in plants treated with 100 M NaCl.

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Abbreviations

AA:

Ascorbic acid

ACC:

1-Aminocyclopropane-1-carboxylic acid

LAI:

Leaf-area index

NED-HCL:

N-(1-Naphthyl) ethylenediamine dihydrochloride

OD:

Optical density

PRO:

Proline

ROS:

Reactive oxygen species

SA:

Salicylic acid

TCA:

Trichloroacetic acid

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Authors and Affiliations

  1. Plant Physiology Section, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Mohd Idrees, M. Naeem, Tariq Aftab, M. Masroor A. Khan &  Moinuddin

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  1. Mohd Idrees
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  2. M. Naeem
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Correspondence to Mohd Idrees.

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Communicated by M. Rapacz.

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Idrees, M., Naeem, M., Aftab, T. et al. Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.) G. Don]. Acta Physiol Plant 33, 987–999 (2011). https://doi.org/10.1007/s11738-010-0631-6

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