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Leaf callus induction and suspension culture establishment in lychee (Litchi chinensis Sonn.) cv. Huaizhi

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Abstract

This study reports a protocol for leaf callus induction and suspension culture establishment in lychee cv. Huaizhi. The results showed that 12-day-old leaf explants cultured under a photoperiod of 16/8 h with their adaxial side touching the medium were the optimum conditions for leaf callus induction. Globular embryos were formed when the induced calli were kept on the callus induction medium without 2,4-D for 24 weeks. Friable calli were induced after 2–3 subcultures at 4 weeks intervals on the Murashige and Skoog medium supplemented with 3 mg/L IAA and 2 mg/L BAP. Suspension culture was established when these friable calli were subcultured six times in liquid callus induction medium.

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Abbreviations

BAP:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KT:

Kinetin

NAA:

α-Naphthalene acetic acid

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Acknowledgments

The work was accomplished at Guangdong Key Laboratory of Vegetable and Fruit. We are thankful to the Fruit Research Institute, Guangdong Academy of Agricultural Science for giving facilities for the present study.

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Author notes

  1. Xin-Ye Ma

    Present address: The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China

Authors and Affiliations

  1. The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, 510275, Guangzhou, People’s Republic of China

    Xin-Ye Ma & Xue-Lin Huang

  2. Fruit Research Institute, Guangdong Academy of Agricultural Science, 510640, Guangzhou, People’s Republic of China

    Gan-Jun Yi & Ji-Wu Zeng

Authors
  1. Xin-Ye Ma
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  2. Gan-Jun Yi
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  3. Xue-Lin Huang
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  4. Ji-Wu Zeng
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Corresponding author

Correspondence to Xue-Lin Huang.

Additional information

Communicated by E. Lojkowska.

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Ma, XY., Yi, GJ., Huang, XL. et al. Leaf callus induction and suspension culture establishment in lychee (Litchi chinensis Sonn.) cv. Huaizhi. Acta Physiol Plant 31, 401–405 (2009). https://doi.org/10.1007/s11738-008-0223-x

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