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UCP2 and PLIN1 Expression Affects the Resting Metabolic Rate and Weight Loss on Obese Patients

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Abstract

Background

Analysis of the expression of genes related to the energy metabolism can elucidate the understanding of physiological and genetic factors that contribute to obesity. This study aimed to evaluate the expression of genes and its influence on resting metabolic rate and weight loss in obese patients before and after bariatric surgery.

Methods

This study was conducted on 23 women, who were divided into two groups: bariatric surgery (preoperative and 6 months after surgery) and control. Abdominal subcutaneous adipose tissue samples were collected to analyze the gene expression, and the resting metabolic rate (RMR) was measured by indirect calorimetry.

Results

Significant differences were observed in weight reduction (22 %, p = 0.01), BMI (22.5 %, p = 0.01), and RMR values (10.5 %, p = 0.01) after the bariatric surgery, while the weight-adjusted RMR increased (15.8 %, p = 0.01). Increased UCP2 expression after 6 months of Roux-en-Y gastric bypass (RYGB) as compared to preoperative period (0.764 to 1.268, p = 0.01) was observed. Analysis with weight-adjusted RMR as dependent variable revealed that UCP2 (r 2 = 0.517, p = 0.01) and PLIN1 (r 2 = 0.420, p = 0.04) expression determine the RMR values in preoperative period. Moreover, UCP2 and PLIN1 expression in preoperative period influenced the percentage of weight loss, even when adjusted for age and BMI.

Conclusions

We have demonstrated that after 6 months of bariatric surgery, there is significant increase in the UCP2 expression. Additionally, the expression of UCP2 and PLIN1 genes influences the resting metabolic rate in obese individuals and could predict the weight loss after bariatric surgery.

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Acknowledgments

This work was supported by grant nos. 2013/06563-7 and 2013/08916-4 from the São Paulo Research Foundation (FAPESP).

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Authors and Affiliations

  1. Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil

    Bruno Affonso Parenti de Oliveira, Marcela Augusta de Souza Pinhel, Carolina Ferreira Nicoletti, Cristiana Cortes de Oliveira, Driele Cristina Gomes Quinhoneiro, Natália Yumi Noronha, Priscila Giácomo Fassini, Wilson Araújo da Silva Júnior, Wilson Salgado Junior & Carla Barbosa Nonino

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  1. Bruno Affonso Parenti de Oliveira
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  2. Marcela Augusta de Souza Pinhel
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  3. Carolina Ferreira Nicoletti
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  4. Cristiana Cortes de Oliveira
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  6. Natália Yumi Noronha
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  9. Wilson Salgado Junior
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  10. Carla Barbosa Nonino
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Correspondence to Carla Barbosa Nonino.

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The authors declare that they have no conflict of interest.

Ethical Approval

All procedures performed in this study were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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de Oliveira, B.A.P., de Souza Pinhel, M.A., Nicoletti, C.F. et al. UCP2 and PLIN1 Expression Affects the Resting Metabolic Rate and Weight Loss on Obese Patients. OBES SURG 27, 343–348 (2017). https://doi.org/10.1007/s11695-016-2275-0

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