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Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings

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Abstract

Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA (indole-3-butyric acid) concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4°C for 0 (immediate planting), 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions: 0 (free of IBA), 1,000, 2,000, 3,000 and 4,000 mg·L−1. Every seven days (0 (immediate planting), 7, 14, 21 and 28 days), destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg·L−1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Exposure to low temperature induced adventitious root formation with diffuse vascular connections.

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Authors and Affiliations

  1. Department of Forest Engineering, Federal University of Mato Grosso (UFMT), Avenida Fernando Corrêa da Costa, 2367, ZC 78060-900, Cuiabá, Mato Grosso, Brazil

    Gilvano Ebling Brondani

  2. Department of Forest Sciences, “Luiz de Queiroz” College of Agriculture, University of São Paulo (ESALQ/USP), Avenida Pádua Dias, 11, POB 9, ZC 13418-900, Piracicaba, São Paulo, Brazil

    Francisco José Benedini Baccarin & Antonio Natal Gonçalves

  3. Department of Biological Sciences, “Luiz de Queiroz” College of Agriculture, University of São Paulo (ESALQ/USP), Avenida Pádua Dias, 11, POB 9, ZC 13418-900, Piracicaba, São Paulo, Brazil

    Heron Wilhelmus de Wit Ondas & Marcilio de Almeida

  4. Department of Forest and Environmental Sciences, North Carolina State University, Carolina, NC, 27695, USA

    José Luiz Stape

Authors
  1. Gilvano Ebling Brondani
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  2. Francisco José Benedini Baccarin
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  3. Heron Wilhelmus de Wit Ondas
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  4. José Luiz Stape
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  5. Antonio Natal Gonçalves
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  6. Marcilio de Almeida
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Corresponding author

Correspondence to Gilvano Ebling Brondani.

Additional information

Foundation project: This work was financially by FAPESP (São Paulo Research Foundation, Brazil) and CAPES (Coordination for the Improvement of Higher Level Personnel, Brazil).

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Brondani, G.E., Baccarin, F.J.B., de Wit Ondas, H.W. et al. Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings. Journal of Forestry Research 23, 583–592 (2012). https://doi.org/10.1007/s11676-012-0298-5

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  • DOI: https://doi.org/10.1007/s11676-012-0298-5

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