Abstract
The objectives of this study were to investigate the effects of successive continuous generation (SCG) cuttings of Buxus sinica var. parvifolia on the rejuvenation of ortets at the hormone level, detect levels of indoleacetic acid (IAA), abscisic acid (ABA), isopentenyladenosine (iPA), zeatin riboside (ZR) and gibberellin4 (GA4) during the rooting process of different generations by means of enzyme-linked immunosorbent assay (ELISA) technology and statistically analyze the rooting characteristics of cuttings. The results show that, the root systems of subsequent generations (generation 1998, 2000) developed satisfactorily, only a few initial generation (generation 1990, 1994) cuttings rooted and the root growth was inhibited. Around the period of callus formation and root projection through the epidermis, there was a marked, regular variation in the levels of IAA, ABA and GA4 between the initial generations and subsequent generations.
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References
Berthon J Y, Maldiney R Sotta B, Gaspar T, Boyer N. 1989. Endogenous levels of plant hormones during the course of adventitious rooting in cuttings of Sequoiadendron giganteum in vitro. Biochem. Physiol. Pfl., 184: 405–411
Blakesley D, Weston G D, Elliott M C. 1991. Endogenous levels of indole-3-acetic acid and abscisic acid during the rooting of Cornus coggygria cuttings taken at different times of the Year. Plant Growth Regul., 10: 1–12
Bollmark M, Kubat B, Eliasson E. 1988. Variation in endogenous cytokinin content during adventitious root formation in pea cuttings. J. Plant Physiol., 132: 262–265
Chen X M, Gao H B, Wang S S. 1994. Studies on endogenous hormone levels in cuttings of three poplar species during rooting process. Sci. Silv. Sin., 30(1): 1–7 (in Chinese with an English abstract)
Ford Y Y, Bonham E C, Cameron R W F, Blake P S, Judd H L, Harrison-Murray R S. 2001. Adventitious rooting: examining the role of auxin in an easy-and a difficult-to-root plant. Plant Growth Regul., 36(2): 149–159
Jarvis B C. 1986. Endogenous control of adventitious rooting in non-woody cuttings. In: Jackson M B (ed), New Root Formation in Plants and Cuttings. Dordrecht: Martinus Nijhoff, 191–223
Ji K S, Wang Z R, Chen T H, Wang M X. 1997. Study on the endogenous inhibitors in Masson pine (Pinus massoniana Lamb.) cuttings. Sci. Silv. Sin., 33(2): 142–151 (in Chinese with an English abstract)
Ji K S, Wang Z R, Chen T H, Wang M X. 1999. Cyclophysis and effect of rejuvenation with continued cottage in Pinus massoniana cutting propagation. J. Zhejiang For. Coll., 16(4): 341–345 (in Chinese with an English abstract)
Kefeli V I. 1978. Natural Plant Growth Inhibitors and Phytohormones. The Hague: Dr W Junk Publisher, 4–38, 133–145
Liu G F, Yang C P, Qu G Z, You X L. 2001. Dynamic changes of four endogenous hormones in the larch hybrid during cutting rooting. J. Northeast For. Univ., 29(6): 1–3 (in Chinese with an English abstract)
Liu G J, Li X Y, You X L, Zhang J, Zhao Y H, Liu G F. 2000. Relationship between endogenous hormone changes of softwood cuttings and adventitious roots of Larix olgensis emerging. J. Northeast For. Univ., 28(1): 19–20 (in Chinese with an English abstract)
Maldiney R, Pelese F, Pilate G, Sotta B, Sossountzov L, Miginiac E. 1986. Endogenous levels of abscisic acid, indole-3-acetic acid, zeatin and zeatin-riboside during the course of adventitious root formation in cuttings of Craigella and Craigella lateral suppressor tomatoes. Physiol. Plant, 68(3): 426–430
Wang J X, Pan R C. 2004. Changes in endogenous hormone contents of Mung beans during adventitious root formation of cuttings. Plant Physiol. Commun., 40(6): 696–698 (in Chinese with an English abstract)
Wang J. 2001. The study on Actinidia cuttage rooting physiology and regulatory mechanisms. Master’s Thesis. Hefei: Anhui Agricultural University (in Chinese with an English abstract)
Wiesman Z, Riov J. 1994. Interaction of paclobutrazol and indole-3-buryric acid in relation to rooting of Mung bean (Vigna radiata) cuttings. Physiol. Plant, 92: 608–612
Xu J Z, Chen S W. 1989. The effect of the changes of the endogenous hormone’s contents (ABA and IAA) in hardwood cuttings of peach to rooting. Acta Hort. Sin., 16(4): 275–278 (in Chinese with an English abstract)
Zhan Y G, Yang C P, Jin Z F, Wang Y C. 2001. Endogenous hormones and nutritive material in softwood cuttings of Betula platyphylla during rooting. J. Northeast For. Univ., 29(4): 1–4 (in Chinese with an English abstract)
Zhang X P, Fang Y M, Huang S H. 2004. The endogenous hormone’s variation during the adventurous foots formation of hybrid tulip trees by cutting. J. Nanjing For. Univ., 28(3): 79–82 (in Chinese with an English abstract)
Zhou S, Deng D F. 1994. Cuttage reproduction of Mexican cypress superior clones. Hunan For. Sci. Tech., 21(3): 39–42, 54 (in Chinese with an English abstract)
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Huang, Y., Ji, Ks. & Zhai, Jr. Relationship between rooting ability and endogenous phytohormone changes in successive continuous generation cuttings of Buxus sinica var. parvifolia, an endangered woody species in China. For. Stud. China 9, 189–197 (2007). https://doi.org/10.1007/s11632-007-0030-2
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DOI: https://doi.org/10.1007/s11632-007-0030-2