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Improved in vitro rooting and acclimatization of Capsicum chinense Jacq. plantlets

  • Micropropagation
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Abstract

In vitro conditions such as low light intensity, the levels of sucrose and other nutrients, and high relative humidity may cause anatomical and physiological changes that have negative effects on acclimatization of regenerated plants. The management of these conditions during in vitro culture could improve the plant ex vitro performance. The influences of light intensity, sucrose concentration, the nutritional composition of the culture medium, and culture container size on in vitro rooting and acclimatization were evaluated in Capsicum chinense cv. Naranja plants. The size of the culture container vessel did not affect plant growth. The best responses of the growth variables tested (plant height, number of leaves, number of roots, dry weight, and osmotic potential) and of survival and rooting were obtained with a photosynthetic photon flux density (PPFD) of 28 μmol m−2 s−1 and H medium with sucrose supplementation at either 1.5% or 3% (w/v). The combination of a PPFD of 28 μmol m−2 s−1 and H medium with 1.5% sucrose can be used for successful rooting of C. chinense adventitious shoots, to enhance the performance of the plantlets in the greenhouse or field and to reduce production costs. The factors studied during in vitro culture significantly influenced ex vitro growth.

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Acknowledgments

The authors would like to express their gratitude to Consejo Nacional de Ciencia y Tecnología (CONACYT, Mexico) for the scholarship awarded to ABL (175327), Fundación “Czeslawa Prywer Lidzbarska,” and Consejo Mexiquense de Ciencia y Tecnología (COMECYT).

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Authors and Affiliations

  1. Posgrado en Recursos Genéticos y Productividad, Colegio de Postgraduados, Km. 36.5, Carretera México-Texcoco, Montecillo, Texcoco, Estado de México, C.P. 56230, Mexico

    A. Barrales-López & A. Robledo-Paz

  2. Posgrado en Botánica, Colegio de Postgraduados, Km. 36.5, Carretera México-Texcoco, Montecillo, Texcoco, Estado de México, C.P. 56230, Mexico

    C. Trejo

  3. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Km. 13.5, Carretera México-Texcoco, C.P. 56250, Coatlinchán, Estado de México, Mexico

    E. Espitia-Rangel

  4. Academia de Genética, Departamento de Fitotecnia, Universidad Autónoma Chapingo, Km. 38.5, Carretera México-Texcoco, Chapingo, Estado de México, C.P. 56230, Mexico

    J. L. Rodríguez-De La O

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  1. A. Barrales-López
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  2. A. Robledo-Paz
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  4. E. Espitia-Rangel
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  5. J. L. Rodríguez-De La O
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Correspondence to A. Robledo-Paz.

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Editor: Neftali Ochoa-Alejo

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Barrales-López, A., Robledo-Paz, A., Trejo, C. et al. Improved in vitro rooting and acclimatization of Capsicum chinense Jacq. plantlets. In Vitro Cell.Dev.Biol.-Plant 51, 274–283 (2015). https://doi.org/10.1007/s11627-015-9671-3

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  • DOI: https://doi.org/10.1007/s11627-015-9671-3

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