Abstract
Nodal segment explants of Ilex paraguariensis, collected from greenhouse-grown plants, were found to contain endophytic bacteria. After culturing in bioreactors, 16 rRNA gene analyses and analytical profile index biochemical tests were used to identify these bacteria as Stenotrophomonas malthophilia. The presence of bacterial cells in the intercellular spaces of stem cortical parenchyma was detected in histological sections by scanning electron microscopy. A range of commercial isothiazolone biocides were tested for their ability to repress the growth of Gram-negative bacteria grown in liquid media during the micropropagation phase. The addition of 0.75 ml l−1 Delcide™ TG (5-chloro-2-methyl-4-isothiazolin-3-one + 2-methyl-4-isothiazolin-3-one, 1.05% and 0.45%, respectively) to the culture media resulted in 100% visibly clean cultures, with no suppression of shoot growth.
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Acknowledgments
The authors are grateful for funding support from SGCyT-UNNE (grant no. 014/10, 005/11), CONICET (PIP 0734), and Establecimiento Las Marías S.A.C.I.F.A. We extend our deepest appreciation to the companies that provided the biocides.
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Editor: David Duncan
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Luna, C., Acevedo, R., Collavino, M. et al. Endophytic bacteria from Ilex paraguariensis shoot cultures: localization, characterization, and response to isothiazolone biocides. In Vitro Cell.Dev.Biol.-Plant 49, 326–332 (2013). https://doi.org/10.1007/s11627-013-9500-5
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DOI: https://doi.org/10.1007/s11627-013-9500-5