Skip to main content
Log in

Differentiation potential of rabbit CD90-positive cells sorted from adipose-derived stem cells in vitro

  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

Abstract

To investigate the differentiation potential of purified CD90+ cells sorted from adipose-derived stem cells (ADSCs), CD90+ cells were sorted from rabbit ADSCs using flow cytometry. Then, cell expansion of CD90+ cells and unsorted ADSCs was observed using an inverted microscope. Furthermore, cell surface markers including CD40, CD105, and CD90 on CD90+ cells and unsorted ADSCs were quantified using flow cytometry. Additionally, multi-lineage differentiation ability between CD90+ cells and unsorted ADSCs was compared, and expression of adipocyte-related genes PPAR-r and CEBPA as well as stem cell-related gene SOX2 in CD90+ cells and unsorted ADSCs was determined using real-time quantitative PCR. We found that CD90+ cells had a stronger cell proliferation ability than unsorted ADSCs. CD90+ cells showed a stronger ability of osteoblast and chondrocyte differentiation than unsorted ADSCs and CD90 cells, whereas the adipose differentiation ability of CD90+ cells was similar to that of ADSCs and CD90 cells. CD14, CD105, and CD90 on CD90+ cells were expressed more highly than those on ADSCs. Additionally, the mRNA expression level of SOX2 in CD90+ cells was significantly higher than that in ADSCs, whereas the expression of PPAR-r and CEBPA was markedly lower than that in ADSCs. These results suggested that the purified CD90+ cells sorted from ADSCs exhibit a stronger differentiation potential than the unsorted ADSCs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Aslan H, Zilberman Y, Kandel L, Liebergall M, Oskouian RJ, Gazit D, Gazit Z (2006) Osteogenic differentiation of noncultured immunoisolated bone marrow-derived CD105+ cells. Stem Cells 24:1728–1737

    Article  PubMed  Google Scholar 

  • Braun J, Hack A, Weis-Klemm M, Conrad S, Treml S, Kohler K, Walliser U, Skutella T, Aicher WK (2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells. Am J Vet Res 71:1228–1236

    Article  PubMed  Google Scholar 

  • Chen XD, Qian HY, Neff L, Satomura K, Horowitz MC (1999) Thy-1 antigen expression by cells in the osteoblast lineage. J Bone Miner Res 14:362–375

    Article  CAS  PubMed  Google Scholar 

  • Chung MT, Liu C, Hyun JS, Lo DD, Montoro DT, Hasegawa M, Li S, Sorkin M, Rennert R, Keeney M (2013) CD90 (Thy-1)-positive selection enhances osteogenic capacity of human adipose-derived stromal cells. Tissue Eng Part A 19:989–997

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Davies OG, Cooper PR, Shelton RM, Smith AJ, Scheven BA (2015) Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation. J Tissue Eng 6:2041731415592356

    Article  PubMed  PubMed Central  Google Scholar 

  • Dennis JE, Esterly K, Awadallah A, Parrish CR, Poynter GM, Goltry KL (2007) Clinical-scale expansion of a mixed population of bone marrow-derived stem and progenitor cells for potential use in bone tissue regeneration. Stem Cells 25:2575–2582

    Article  PubMed  Google Scholar 

  • Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement Cytotherapy 8:315–317

    CAS  PubMed  Google Scholar 

  • García-Contreras M, Vera-Donoso CD, Hernandez-Andreu JM, García-Verdugo JM, Oltra E (2014) Therapeutic potential of human adipose-derived stem cells (ADSCs) from cancer patients: a pilot study. PLoS One 9:e113288

    Article  PubMed  PubMed Central  Google Scholar 

  • Gimble J, Rad MR, Yao S (2013) Adipose tissue-derived stem cells and their regeneration potential. Stem Cells Craniofac Dev Regen 241–258

  • Golipoor Z, Ragerdi Kashani I, Akbari M, Hassanzadeh G, Malek F, Mahmoudi R (2010) Differentiation of adipose-derived stem cells into schwann cell phenotype in comparison with bone marrow stem cells. Iranian Journal of Basic Medical Sciences 13:76–84

    CAS  Google Scholar 

  • Hosoya A, Hiraga T, Ninomiya T, Yukita A, Yoshiba K, Yoshiba N, Takahashi M, Ito S, Nakamura H (2012) Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells. Histochem Cell Biol 137:733–742

    Article  CAS  PubMed  Google Scholar 

  • Ishimura D, Yamamoto N, Tajima K, Ohno A, Yamamoto Y, Washimi O, Yamada H (2008) Differentiation of adipose-derived stromal vascular fraction culture cells into chondrocytes using the method of cell sorting with a mesenchymal stem cell marker. Tohoku J Exp Med 216:149–156

    Article  PubMed  Google Scholar 

  • Jiang T, Liu W, Lv X, Sun H, Zhang L, Liu Y, Zhang WJ, Cao Y, Zhou G (2010) Potent in vitro chondrogenesis of CD105 enriched human adipose-derived stem cells. Biomaterials 31:3564–3571

    Article  CAS  PubMed  Google Scholar 

  • Krawetz RJ, Wu YE, Martin L, Rattner JB, Matyas JR, Hart DA (2012) Synovial fluid progenitors expressing CD90+ from normal but not osteoarthritic joints undergo chondrogenic differentiation without micro-mass culture. PLoS One 7:e43616

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu K, Lin B, Zhao M, Yang X, Chen M, Gao A, Liu F, Que J, Lan X (2013) The multiple roles for Sox2 in stem cell maintenance and tumorigenesis. Cell Signal 25:1264–1271

    Article  CAS  PubMed  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  • Miranville A, Heeschen C, Sengenes C, Curat C, Busse R, Bouloumie A (2004) Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation 110:349–355

    Article  CAS  PubMed  Google Scholar 

  • Mitterberger MC, Lechner S, Mattesich M, Kaiser A, Probst D, Wenger N, Pierer G, Zwerschke W (2012) DLK1 (PREF1) is a negative regulator of adipogenesis in CD105+/CD90+/CD34+/CD31−/FABP4− adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women. Stem Cell Res 9:35–48

    Article  CAS  PubMed  Google Scholar 

  • Niemelä S, Miettinen S, Sarkanen J, Ashammakhi N (2008) Adipose tissue and adipocyte differentiation: molecular and cellular aspects and tissue engineering applications. Topics in Tissue Engineering 4:26

    Google Scholar 

  • Ramirez-Zacarias J, Castro-Munozledo F, Kuri-Harcuch W (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97:493–497

    Article  CAS  PubMed  Google Scholar 

  • Reich CM, Raabe O, Wenisch S, Bridger PS, Kramer M, Arnhold S (2012) Isolation, culture and chondrogenic differentiation of canine adipose tissue-and bone marrow-derived mesenchymal stem cells—a comparative study. Vet Res Commun 36:139–148

    Article  PubMed  Google Scholar 

  • Shin S, Kim Y, Jeong S, Hong S, Kim I, Lee W, Choi S (2013) The therapeutic effect of human adult stem cells derived from adipose tissue in endotoxemic rat model. International journal of medical sciences 10:8

    Article  CAS  PubMed  Google Scholar 

  • Yamamoto M, Nakata H, Hao J, Chou J, Kasugai S, Kuroda S (2014) Osteogenic potential of mouse adipose-derived stem cells sorted for CD90 and CD105 in vitro. Stem Cells Int 2014:1–17

    Article  Google Scholar 

  • Yanez R, Lamana ML, García-Castro J, Colmenero I, Ramírez M, Bueren JA (2006) Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease. Stem Cells 24:2582–2591

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Core Facilities of Zhejiang University School of Medicine for technical assistance. This work was supported by the Project of Science and Technology Department of Zhejiang Province (no. 2014C37067, no. 2015C37008), the National Natural Science Foundation of China (no. 81501943), the Fundamental Research Funds for the Central Universities (no. 2016QNA7021), and the Important Subject Fund of Science Technology Department of Zhejiang province (no. 2013C03048-1).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ting Shen.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Editor: Tetsuji Okamoto

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Song, X., Hong, C., Zheng, Q. et al. Differentiation potential of rabbit CD90-positive cells sorted from adipose-derived stem cells in vitro. In Vitro Cell.Dev.Biol.-Animal 53, 77–82 (2017). https://doi.org/10.1007/s11626-016-0081-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11626-016-0081-6

Keywords

Navigation