Skip to main content
Log in

A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice

  • Letter to the Editor
  • Published:
Science China Life Sciences Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Bryan, G.T., Wu, K.S., Farrall, L., Jia, Y., Hershey, H.P., McAdams, S.A., Faulk, K.N., Donaldson, G.K., Tarchini, R., and Valent, B. (2000). tA single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell 12, 2033–2046.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiao, Y., Wang, Y., Xue, D., Wang, J., Yan, M., Liu, G., Dong, G., Zeng, D., Lu, Z., Zhu, X., Qian, Q., and Li, J. (2010). Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet 42, 541–544.

    Article  CAS  PubMed  Google Scholar 

  • Komor, A.C., Kim, Y.B., Packer, M.S., Zuris, J.A., and Liu, D.R. (2016). Programmable editing of a target base in genomic DNA without doublestranded DNA cleavage. Nature 533, 420–424.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ma, X., Zhu, Q., Chen, Y., and Liu, Y.G. (2016). CRISPR/Cas9 platforms for genome editing in plants: developments and applications. Mol Plant 9, 961–974.

    Article  CAS  PubMed  Google Scholar 

  • Zhou, H., Liu, B., Weeks, D.P., Spalding, M.H., and Yang, B. (2014). Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice. Nucleic Acids Res 42, 10903–10914.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We thank Xiaonan Zhao, Jining Normal University, for technical assistance; Bo Ding and Qiuyin Yang for critically reading the manuscript. This work was supported by the Agricultural Science and Technology Innovation Program of The Chinese Academy of Agricultural Sciences to Huanbin Zhou, and Open Project of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, to Huanbin Zhou and Dawei Zhang.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Honghui Lin or Huanbin Zhou.

Additional information

Contributed equally to this work.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ren, B., Yan, F., Kuang, Y. et al. A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice. Sci. China Life Sci. 60, 516–519 (2017). https://doi.org/10.1007/s11427-016-0406-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-016-0406-x

Navigation