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Influence of UV-A radiation on oxidative stress and antioxidant enzymes in Mythimna separata (Lepidoptera: Noctuidae)

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Abstract

Abiotic stress factors, including ultraviolet (UV) radiation, significantly affect insect life. UV-A radiation (320–400 nm) has been widely used for insect control since it increases the production of ROS and causes oxidative cell damage. In the present study, we evaluated the effects of UV-A irradiation on an important pest in China, the ear-cutting caterpillar, Mythimna separata (Lepidoptera: Noctuidae). We exposed 3-day-old M. separata adults to UV-A radiation for different periods of time (0, 30, 60, 90, and 120 min) and evaluated the resulting total antioxidant capacity and the activity of the antioxidant enzymes superoxide dismutase, catalase, peroxidase, and glutathione-S-transferase. The total antioxidant capacity significantly increased after exposure to UV-A radiation for 60 min but decreased after 90 and 120 min of exposure, compared with the control. The antioxidant activity of glutathione-S-transferase, superoxide dismutase, catalase, and peroxidase increased after 60-min exposure, and it was decreased at the longest exposure period 120 min. The longest exposure time period relatively activates the xenobiotic detoxifying enzymes like glutathione-S-transferase, superoxide dismutase, catalase, and peroxidase enzymes. The longest duration of UV-A radiation may cooperate with pesticide detoxification mechanism in insects, making them more susceptible to insecticides. Our results demonstrated that UV irradiation causes oxidative stress, affects the activity of antioxidant enzymes, and disturbs the physiology of M. separata adults.

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Acknowledgments

This study was supported by the National Department Public Benefit (Agriculture) Research Foundation (201403031) and the National Natural Science Foundation of China (No. 30871639).

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Authors and Affiliations

  1. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China

    Arif Ali, Muhammad Adnan Rashid, Qiu Ying Huang & Chao-Liang Lei

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  1. Arif Ali
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  2. Muhammad Adnan Rashid
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  4. Chao-Liang Lei
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Correspondence to Chao-Liang Lei.

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Ali, A., Rashid, M.A., Huang, Q.Y. et al. Influence of UV-A radiation on oxidative stress and antioxidant enzymes in Mythimna separata (Lepidoptera: Noctuidae). Environ Sci Pollut Res 24, 8392–8398 (2017). https://doi.org/10.1007/s11356-017-8514-7

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