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Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel

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Abstract

In the current study, sweet potato peel (Ipomoea batatas) was observed as the most favorable substrate for the maximum synthesis of α-1,4-glucosidase among various agro-industrial residues. Bacillus licheniformis KIBGE-IB4 produced 6533.0 U ml−1 of α-1,4-glucosidase when growth medium was supplemented with 1% dried and crushed sweet potato peel. It was evident from the results that bacterial isolate secreted 6539.0 U ml−1 of α-1,4-glucosidase in the presence of 0.4% peptone and meat extract with 0.1% yeast extract. B. licheniformis KIBGE-IB4 released 6739.0 and 7190.0 U ml−1 of enzyme at 40 °C and pH 7.0, respectively. An improved and cost-effective growth medium design resulted 8590.0 U ml−1 of α-1,4-glucosidase with 1.3-fold increase as compared to initial amount from B. licheniformis KIBGE-IB4. This enzyme can be used to fulfill the accelerating demand of food and pharmaceutical industries. Further purification and immobilization of this enzyme can also enhance its utility for various commercial applications.

Pictorial representation of maltase production from sweet potato peel

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Acknowledgements

This research work was financially supported by the Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi-75270, Pakistan.

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Authors and Affiliations

  1. Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir Upper, KPK, Pakistan

    Muhammad Asif Nawaz

  2. The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan

    Muhammad Asif Nawaz, Afsheen Aman & Shah Ali Ul Qader

  3. Department of Biotechnology, Federal Urdu University of Arts, Science, and Technology, Karachi, Pakistan

    Zainab Bibi

  4. National Institute of Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan

    Asad Karim

  5. Department of Chemistry, University of Turbat, Kech, Balochistan, Pakistan

    Haneef Ur Rehman

  6. Department of Microbiology, Abdul Wali Khan University, Garden Campus, Mardan, Pakistan

    Muhsin Jamal

  7. Department of Botany, University of Malakand, Chaldara, KPK, Pakistan

    Tour Jan

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  1. Muhammad Asif Nawaz
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  2. Zainab Bibi
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Responsible editor: Philippe Garrigues

Highlights

• Various agro-industrial wastes and pure substrates were evaluated as feedstock for α-1,4-glucosidase production.

• α-1,4-Glucosidase production was optimized using sweet potato peel as a sole carbon source.

• Incorporation of organic nitrogen sources in growth medium promoted the enzyme synthesis.

• Broad thermal and pH stability profile indicated its utility in various industrial bioprocesses.

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Nawaz, M.A., Bibi, Z., Karim, A. et al. Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel. Environ Sci Pollut Res 24, 4058–4066 (2017). https://doi.org/10.1007/s11356-016-8168-x

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