Skip to main content
Log in

Effect of Telmisartan on local cardiovascular oxidative stress in mouse under chronic intermittent hypoxia condition

  • Original Article
  • Published:
Sleep and Breathing Aims and scope Submit manuscript

An Erratum to this article was published on 15 September 2012

Abstract

Objective

The objective of this study is to explore the protective effect of an angiotensin receptor blocker (ARB) on local cardiovascular angiotensin II (AngII) and oxidative stress markers such as malondialdehyde (MDA), NADPH oxydase p47phox, and 8-hydroxy-2′-deoxyguanosine/8-hydroxyguanosine (8-OHdG/8-OHG) of mice that had chronic intermittent hypoxia (CIH).

Methods

Thirty-two healthy male C57B6J mice were randomly divided into four groups: CIH (12 weeks of CIH), ARB (CIH+Telmisartan), air control (room air delivery), and blank control (no treatment). AngII, p47phox, and 8-OHdG/8-OHG were detected in mouse cardiocytes by immunohistochemistry. MDA was measured with the thiobarbituric acid method.

Measurements and results

The highest AngII levels occur in the ARB treatment group (P < 0.05), followed by the CIH group (which was higher than the two control groups; P = 0.000). The levels of p47phox were statistically higher in the CIH group than in the other groups (P = 0.000) and lower in the ARB group (but still higher than in the two control groups, P = 0.000). The levels of 8-OHdG/8-OHG were the highest in the CIH group (P < 0.05), followed by the ARB group (which was higher than the two control groups, P = 0.000). The levels of MDA in the myocardial homogenate of mice were the highest in the CIH group (P = 0.000).

Conclusions

Based on the above results, it can be concluded that Telmisartan may protect mouse cardiocytes from oxidative stress damage due to CIH.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Yang Song-qing, Han Li-li, Dong Xiao-lu, Wang Chun-yong, Xia H, Liu P, Wang Jing-hua, He Ping-ping, Liu Sheng-nan, Li Ming-xian (2011) Mal-effects of obstructive sleep apnea on the heart. Sleep Breath. doi:10.1007/s11325-011-0566-1

  2. Shah NA, Yaggi HK, Concato J, Mohsenin V (2010) Obstructive sleep apnea as a risk factor for coronary events or cardiovascular death. Sleep Breath 14(2):131–136

    Article  PubMed  Google Scholar 

  3. Christou K, Markoulis N, Moulas AN, Pastaka C, Gourgoulianis KI (2003) Reactive oxygen metabolites (ROMs) as an index of oxidative stress in obstructive sleep apnea patients. Sleep Breath 7:105–110

    Article  PubMed  Google Scholar 

  4. Vatansever E, Surmen-Gur E, Ursavas A, Karadag M (2010) Obstructive sleep apnea causes oxidative damage to plasma lipids and proteins and decreases adiponectin levels. Sleep Breath 15(3):275–282

    Article  PubMed  Google Scholar 

  5. Xiao-yang CHEN, Yi-ming Z, Zi-yang H, Geng T, Xing-zhong Z, Yong-jia L (2005) Effect of chronic intermittent hypoxia on the expression of HIF-1α. Chinese Journal of Tuberculosis and Respiratory Diseases 28(2):93–96

    Google Scholar 

  6. Nagai N, Oike Y, Noda K, Urano T, Kubota Y, Ozawa Y, Shinoda H, Koto T, Shinoda K, Inoue M, Tsubota K, Yamashiro K, Suda T, Ishida S (2005) Suppression of ocular inflammation in endotoxin-induced uveitis by blocking the angiotensin II type 1 receptor. Invest Ophthalmol Vis Sci 46(8):2925–2931

    Article  PubMed  Google Scholar 

  7. Victor Chi, Chandy George K (2007) Immunohistochemistry: paraffin sections using the Vectastain ABC Kit from Vector Labs. J Vis Exp 8:308

    Google Scholar 

  8. Sinnhuber RO, Yu TC, Yu TCE (1958) Characterization of the red pigment formed in the 2-thiobarbituric acid determination of oxidative rancidity. Food Research 23:626–633

    Article  CAS  Google Scholar 

  9. Foster GE, Poulin MJ, Hanly PJ (2007) Intermittent hypoxia and vascular function: implications for obstructive sleep apnoea. Experimental Physiology 92(1):51–65

    Article  PubMed  Google Scholar 

  10. Tuteja N, Singh MB, Misra MK, Bhalla PL, Tuteja R (2001) Molecular mechanisms of DNAdamage and repair: progress in plants. Crit Rev Biochem Mol Biol 36:337–397

    Article  PubMed  CAS  Google Scholar 

  11. Rey S, Valdés G, Iturriaga R (2007) Pathophysiology of obstructive sleep apnea-associated hypertension. Rev Med Chil 135(10):1333–1342

    PubMed  CAS  Google Scholar 

  12. Hayashi T, Yamashita C, Matsumoto C, Kwak C-J, Fujii K, Hirata T, Miyamura M, Mori T, Ukimura A, Okada Y, Matsumura Y, Kitaura Y (2008) Role of gp91phox-containing NADPH oxidase in left ventricular remodeling induced by intermittent hypoxic stress. Am J Physiol Heart Circ Physiol 294:2197–2203

    Article  Google Scholar 

  13. Ferrario CM, Trask AJ, Jessup JA (2005) Advances in biochemical and functional roles of angiotensin-converting enzyme 2 and angiotensin-(1–7) in regulation of cardiovascular function. Am J Physiol 289:2281–2290

    Google Scholar 

  14. Ferrario CM, Jessup J, Chappell MC, Averill DB, Brosnihan KB, Tallant EA, Diz DI, Gallagher PE (2005) Effect of angiotensin-converting enzyme inhibition and angiotensin II receptor blockers on cardiac angiotensin-converting enzyme 2. Circulation 111:2605–2610

    Article  PubMed  CAS  Google Scholar 

  15. Joergen O, Dendorfer A, Dominiak P (2004) Cardiovascular and renal function of angiotensin II type-2 receptor. Cardiovascular Res 62:460–467

    Article  Google Scholar 

  16. Yamashita C, Hayashi T, Mori T, Tazawa N, Kwak CJ, Nakano D, Sohmiya K, Okada Y, Kitaura Y, Matsumura Y (2007) Angiotensin II receptor blocker reduces oxidative stress and attenuates hypoxia-induced left ventricular remodeling in apolipoprotein E-knockout mice. Hypertens Res 30:1219–1230

    Article  PubMed  CAS  Google Scholar 

  17. Fiala ES, Conaway CC, Mathis JE (1989) Oxidative DNA and RNA damage in the livers of Sprague Dawley rats treated with the hepatocarcinogen 2-nitropropane. Cancer Res 49:5518–5522

    PubMed  CAS  Google Scholar 

  18. Rhee Y, Valentine MR, Termini J (1995) Oxidative base damage in RNA detected by reverse transcriptase. Nucleic Acids Res 23:3275–3282

    Article  PubMed  CAS  Google Scholar 

  19. Wamer WG, Wei RR (1997) In vitro photooxidation of nucleic acids by ultraviolet A radiation. Photochem Photobiol 65:560–563

    Article  PubMed  CAS  Google Scholar 

  20. Wamer WG, Yin JJ, Wei RR (1997) Oxidative damage to nucleic acids photosensitized by titanium dioxide. Free Radic Biol Med 23:851–858

    Article  PubMed  CAS  Google Scholar 

  21. Dani SU (1997) Molecular turnover and aging. In: Dani SU, Hori A, Walter GF (eds) Principles of neural aging. Elsevier, Amsterdam, pp. 83–101

  22. Lavie L, Lavie P (2009) Molecular mechanisms of cardiovascular disease in OSAHS: the oxidative stress link. Eur Respir J 33:1467–1484

    Article  PubMed  CAS  Google Scholar 

  23. Cassarino DS, Bennett JP Jr (1999) An evaluation of the role of mitochondria in neurodegenerative diseases: mitochondrial mutations and oxidative pathology, protective nuclear responses, and cell death in neurodegeneration. Brain Res Brain Res Rev 29:1–25

    Article  PubMed  CAS  Google Scholar 

  24. Romero FJ, Bosch-Morell F, Romero MJ, Jareño EJ, Romero B, Marín N, Romá J (1998) Lipid peroxidation products and antioxidants in human disease. Environ Health Perspect 106(5):1229–1234

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from Fujian Medical University (Number 09ZD015).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yi-Ming Zeng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zeng, YM., Wan, WY., Chen, XY. et al. Effect of Telmisartan on local cardiovascular oxidative stress in mouse under chronic intermittent hypoxia condition. Sleep Breath 17, 181–187 (2013). https://doi.org/10.1007/s11325-012-0669-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11325-012-0669-3

Keywords

Navigation