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Cloning and characterization of two allelic glyceraldehyde-3-phosphate dehydrogenase genes in Auricularia auricula-judae

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Abstract

Two allelic variants of the gpd gene, Gpd a and Gpd b, were isolated based on a putative glyceraldehyde-3-phosphate dehydrogenase encoding sequence from the transcriptome of Auricularia auricula-judae strain Au916. The two alleles were found to have a 73 bp length discrepancy and 39 SNP variations. Both of the genomic DNA sequences of two alleles were interrupted by five introns, and encoded a same 340 aa protein. Intron positions analysis showed that the first intron was absent, but the last unique intron was gained in A. auricula-judae. Allele-specific expression analysis showed that the Gpd a and Gpd b were expressed with no significant difference in dikaryotic mycelia of A. auricula-judae. To the best of our knowledge, this is the first report about the detection of two allelic gpd genes in A. auricula-judae, as well as the application of allele-specific primers in gene expression analysis for this edible fungus.

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Acknowledgments

This work was supported by the Industry (Agriculture), Science and Technology Plans of China (Grant No. nyhyzx07-008) and the key program from the Natural Science Foundation of Hubei province. The authors are grateful to Dr. Changhe Zhou (Virginia Tech.) and Dr. Hua Jiang (Swedish University of Agricultural Sciences) for linguistic revision and suggestions.

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Authors and Affiliations

  1. Institute of Applied Mycology, Huazhong Agricultural University, No. 1 Shizishan Rd., Wuhan, 430070, Hubei, China

    Xiuzhi Fan, Yan Zhou, Yang Xiao & Yinbing Bian

  2. Key Laboratory of Agro-Microbial Resource and Development, Ministry of Agriculture, Wuhan, China

    Xiuzhi Fan, Yan Zhou, Yang Xiao & Yinbing Bian

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  1. Xiuzhi Fan
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  2. Yan Zhou
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  3. Yang Xiao
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Correspondence to Yinbing Bian.

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Fan, X., Zhou, Y., Xiao, Y. et al. Cloning and characterization of two allelic glyceraldehyde-3-phosphate dehydrogenase genes in Auricularia auricula-judae . World J Microbiol Biotechnol 30, 181–189 (2014). https://doi.org/10.1007/s11274-013-1436-8

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  • DOI: https://doi.org/10.1007/s11274-013-1436-8

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