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Antifouling and antibacterial compounds from a marine fungus Cladosporium sp. F14

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Abstract

To investigate the antifouling secondary metabolites from marine-derived fungi, we used bioassay-guided column chromatography techniques, such as HPLC, to separate and purify compounds from Cladosporium sp. F14. Extensive spectral analyses including 1D NMR spectra and MS were employed for structure elucidation of the compounds. Antilarval activity of the compounds was evaluated in settlement inhibition assays with laboratory-reared Balanus amphitrite and Bugula neritina larvae, while antibacterial activity was assessed with disc diffusion bioassay on growth inhibition of six marine bacterial species. In total, nine compounds were obtained. Among them, 3-phenyl-2-propenoic acid, cyclo-(Phe-Pro) and cyclo-(Val-Pro) had various antibacterial activities against three fouling bacteria, furthermore, 3-phenyl-2-propenoic acid and bis(2-ethylhexyl)phthalate effectively inhibited larval settlement of B. neritina and B. amphitrite larvae, respectively, indicating that the two compounds are potential natural antifouling agents.

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Acknowledgements

The authors are grateful to the Knowledge Innovation Program of Chinese Academy of Science (Grant KZCX2–YW-216-1), the CAS/SAFEA International Partnership Program for Creative Research Teams, Knowledge Innovation Program of South China Sea Institute of Oceanology, Chinese Academy of Sciences (Grant LYQY200703), and Science Foundation of Guangdong Province (Grant 8151030101000020) for financial support.

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Authors and Affiliations

  1. Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, The Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, Guangdong, People’s Republic of China

    Shu-Hua Qi & Si Zhang

  2. Coastal Marine Laboratory, Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, People’s Republic of China

    Ying Xu & Pei-Yuan Qian

  3. South-Central University for Nationalities, 708 Mingyuan Road, Wuhan, 430074, People’s Republic of China

    Hai-Rong Xiong

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  1. Shu-Hua Qi
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  2. Ying Xu
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  3. Hai-Rong Xiong
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  4. Pei-Yuan Qian
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  5. Si Zhang
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Correspondence to Shu-Hua Qi.

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Qi, SH., Xu, Y., Xiong, HR. et al. Antifouling and antibacterial compounds from a marine fungus Cladosporium sp. F14. World J Microbiol Biotechnol 25, 399–406 (2009). https://doi.org/10.1007/s11274-008-9904-2

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  • DOI: https://doi.org/10.1007/s11274-008-9904-2

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