Abstract
Eight bacterial strains designated ARI 1-8 were isolated from soil and water samples collected from selenium-contaminated sites in India using the enrichment culture technique. They exhibited very high MIC values ranging from 300 to 750 mM for different forms of selenium (selenite and selenate). On the basis of various biochemical tests, fatty acid methyl ester profile and 16S rRNA gene sequencing, these isolates were identified belonging to the classes β-Proteobacteria and Bacilli. These microorganisms could be further used for bioremediation of contaminated sites.
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References
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Brim H, Osborne JP, Kostandarithes HM, Fredrickson JK, Wackett LP, Daly MJ (2006) Deinococcus radiodurans engineered for complete toluene degradation facilitates Cr(VI) reduction. Microbiology 152:2469–2477
Burton GA Jr, Giddings TH, DeBrine P, Fall R (1987) High incidence of selenite-resistant bacteria from a site polluted with selenium. Appl Environ Microbiol 53:185–188
de Souza MP, Amini A, Dojka MA, Pickering IJ, Dawson SC, Pace NR, Terry N (2001) Identification and characterization of bacteria in a selenium-contaminated hypersaline evaporation pond. Appl Environ Microbiol 67:3785–3794
Ellis RJ, Morgan P, Weightman AJ, Fry JC (2003) Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil. Appl Environ Microbiol 69:3223–3230
Frankenberger WT Jr, Arshad M (2001) Bioremediation of selenium-contaminated sediments and water. Biofactors 14:241–254
Garbisu C, Gonzalez S, Yang WH, Tee BC, Carlson DE, Yee A, Smith N, Otero R, Buchanan BB, Leighton T (1995) Physiological mechanism regulating the conversion of selenate to elemental selenium by B. subtilis. Biofactors 9:29–37
Ghosh A, Paul D, Prakash D, Mayilraj S, Jain RK (2006) Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. Int J Syst Evol Microbiol 56:1965–1969
Haq R, Zaidi SK, Shakoori AR (1999) Cadmium resistant Enterobacter cloacae and Klebsiella sp. isolated from industrial effluents and their possible role in cadmium detoxification. World J Microbiol Biotechnol 15:283–290
Klonowska A, Heulin T, Vermeglio A (2005) Selenite and tellurite reduction by Shewanella oneidensis. Appl Environ Microbiol 71:5607–5609
Kozdroj J, van Elsas JD (2001) Structural diversity of microorganisms in chemically perturbed soil assessed by molecular and cytochemical approaches. J Microbiol Methods 43:197–212
Krieg NR, Holt JG, Murray RGE (eds) (1984) Bergey’s manual of systematic bacteriology, vols. 1 and 2. Williams and Wilkins, Baltimore
Lortie L, Gould WD, Rajan S, McCready RG, Cheng KJ (1992) Reduction of selenate and selenite to elemental selenium by a Pseudomonas stutzeri isolate. Appl Environ Microbiol 58:4042–4044
Losi ME, Frankenberger WT (1997) Reduction of selenium oxyanions by Enterobacter cloacae SLD1a-1: isolation and growth of the bacterium and its expulsion of selenium particles. Appl Environ Microbiol 63:3079–3084
Mokashi SA, Paknikar KM (2002) Arsenic (III) oxidizing Microbacterium lacticum and its use in the treatment of arsenic contaminated groundwater. Lett Appl Microbiol 34:258–262
Nies DH (1999) Microbial heavy-metal resistance. Appl Microbiol Biotechnol 51:730–750
Pandey KK, Mayilraj S, Chakrabarti T (2002) Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567
Rech SA, Macy JM (1992) The terminal reductases for selenate and nitrate respiration in Thauera selenatis are two distinct enzymes. J Bacteriol 174:7316–7320
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Stadtman TC (1990) Selenium biochemistry. Annu Rev Biochem 59:111–127
Stolz JF, Basu P, Santini JM, Oremland RS (2006) Arsenic and selenium in microbial metabolism. Annu Rev Microbiol 60:107–130
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Van de Peer Y, De Wachter R (1994) TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
Wang CL, Maratukulam PD, Lum AM, Clark DS, Keasling JD (2000) Metabolic engineering of an aerobic sulfate reduction pathway and its application to precipitation of cadmium on the cell surface. Appl Environ Microbiol 66:4497–4502
Acknowledgements
We express gratitude to Mr. Mayilraj for his help in carrying out the FAMEs analysis. We thank Mr. Dhan Prakash and Ms. Meenu Khurana for their excellent technical assistance. We are also thankful to Mr. Janmejay Pandey and Mr. Narinder K. Sharma for reading the manuscript. This work was supported, in parts, by CSIR and DBT, Government of India. A.G. acknowledges research fellowship awarded by CSIR. This is IMTECH communication number 014/2007.
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Ghosh, A., Mohod, A.M., Paknikar, K.M. et al. Isolation and characterization of selenite- and selenate-tolerant microorganisms from selenium-contaminated sites. World J Microbiol Biotechnol 24, 1607–1611 (2008). https://doi.org/10.1007/s11274-007-9624-z
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DOI: https://doi.org/10.1007/s11274-007-9624-z