Skip to main content
SpringerLink
Log in

Impact of Bacillus subtilis JA, a biocontrol strain of fungal plant pathogens, on arbuscular mycorrhiza formation in Zea mays

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

In the present study, the influence of Bacillus subtilis JA on arbuscular mycorrhizal fungi (AMF) was evaluated by either pot culture or in vitro conditions, respectively. Under the pot culture conditions, the inoculation of B. subtilis JA decreased the frequency (% F) of the root colonization by indigenous arbuscular mycorrhizal fungi and the shoot dry weight of maize (Zea mays L.), but had no apparent effect on the intensity (% I) of AM fungal root colonization. The unknown volatile emitted from the B. subtilis JA in vitro significantly inhibited spore germination and the hyphal growth in the dual-compartment experiments. Moreover, the data from the direct interaction between B. subtilis JA and Glomus etunicatum showed that soluble antifungal lipopeptides influenced the development of AMF. Therefore, the application of antifungal Bacillus strains should take the compatibility with the indigenous beneficial fungi into consideration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Barea JM, Andrade G, Bianciotto V et al (1998) Impact on arbuscular mycorrhiza formation of Pseudomonas strains used as inoculants for biocontrol of soil-borne fungal plant pathogens. Appl Environ Microbiol 64:2304–2307

    CAS  Google Scholar 

  • Citernesi AS, Filippi C, Bagnoli G, Giovannetti M (1994) Effects of the antimycotic molecule iturin A2, secreted by Bacillus subtilis strain M51, on arbuscular mycorrhizal fungi. Mycol Res 149:241–246

    CAS  Google Scholar 

  • Declerck S, Strullu DG, Plenchette C (1996) In vitro mass-production of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots. Mycol Res 100:1237–1242

    Article  Google Scholar 

  • Declerck S, Strullu DG, Plenchette C (1998) Monoxenic culture of the intraradical forms of Glomus sp. isolated from a tropical ecosystem: a proposed methodology for germplasm collection. Mycologia 90:579–585

    Article  Google Scholar 

  • Doner LW, Bécard G (1991) Solubilization of gellan gels by chelation of cations. Biotechnol Tech 5:25–29

    Article  CAS  Google Scholar 

  • Garbaye J (1994) Helper bacteria: a new dimension to the mycorrhizal symbiosis. New Phytol 128:197–210

    Article  Google Scholar 

  • Koske RE, Gemma JN (1989) A modified procedure for staining roots to detect VA mycorrhizas. Mycol Res 92:486–488

    Google Scholar 

  • Liu J, Liu M, Wang J et al (2005) Enhancement of the Gibberella zeae growth inhibitory lipopeptides from a Bacillus subtilis mutant by ion beam implantation. Appl Microbiol Biotechnol 69:223–238

    Article  CAS  Google Scholar 

  • Liu J, Wang J, Yao JM et al (2004) Properties of the crude extract of Bacillus subtilis and purification of antimicrobial peptides. Acta Microbiol Sin 44:511–514

    CAS  Google Scholar 

  • Pawlowska TE, Douds DD, Charvat I (1999) In vitro propagation and life cycle of the arbuscular mycorrhizal fungus Glomus etunicatum. Mycol Res 103:1549–1556

    Article  Google Scholar 

  • Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic Press, San Diego, CA, USA

    Google Scholar 

  • Sonenshein AL, Hoch JA, Losick R (2001) Bacillus subtilis and its closest relatives. from genes to cells. American Society for Microbiology Press, Washington, DC

    Google Scholar 

  • Stein T (2005) Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol 56:845–857

    Article  CAS  Google Scholar 

  • Toro M, Azcon R, Barea JM (1997) Improvement of arbuscular mycorrhiza development by inoculation of soil with phosphate-solubilizing rhizobacteria to improve rock phosphate bioavailability (32P) and nutrient cycling. Appl Environ Microbiol 63:4408–4412

    CAS  Google Scholar 

  • Vivas A, Marulanda A, Manuel JRL et al (2003) Influence of a Bacillus sp. on physiological activities of two arbuscular mycorrhizal fungi and on plant responses to PEG-induced drought stress. Mycorrhiza 13:249–256

    Article  Google Scholar 

Download references

Acknowledgements

This study was supported by the grant under Hefei Institutes of Physical Science. We would like to thank Dr. Ann Xu for her efforts to make the manuscript fluent.

Author information

Authors and Affiliations

  1. Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, P. O. Box 1126, Hefei, Anhui, 230031, P.R. China

    Xiang Xiao, Hao Chen, Hua Chen, Jun Wang, Chong Ren & Lijun Wu

Authors
  1. Xiang Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hua Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chong Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lijun Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lijun Wu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xiao, X., Chen, H., Chen, H. et al. Impact of Bacillus subtilis JA, a biocontrol strain of fungal plant pathogens, on arbuscular mycorrhiza formation in Zea mays . World J Microbiol Biotechnol 24, 1133–1137 (2008). https://doi.org/10.1007/s11274-007-9584-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-007-9584-3

Keywords

Search

Navigation