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Toxic Effects, Oxidative Stress and Ultrastructural Changes in Moss Taxithelium Nepalense (Schwaegr.) Broth. Under Chromium and Lead Phytotoxicity

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Abstract

The effect of short-term Pb and Cr (0, 100 and 1000μM) stress in moss Taxithelium nepalense (Schwaegr.) Broth., the possible generation of oxidative stress, antioxidant metabolism and changes in the chloroplast and cell membrane ultrastructure were investigated. In moss cells, treatment of Pb and Cr for 12 and 24 h decreased the dry mass and total chlorophyll content with marked inhibition under Pb. Both Pb and Cr accumulated after 24 h of their treatment where highest accumulation of Pb was visible than that of Cr. The ultrastructural studies at 1000 μM of Pb and Cr showed distortion of the thylakoid, distortion of chloroplast membrane and changes in the chloroplast structure. Chloroplast distortion was highly visible under Pb than that of Cr. The distortion in the cell membrane was evident at high concentration of Pb, while under Cr, minor changes were visible as compared to controls. Both Pb and Cr significantly increased the production of ROS like H2O2 and O2− radical with marked production after 24 h under Pb than that of Cr. The alteration in metabolism of activated oxygen in moss cells was evidenced by the increase in the lipid peroxidation in moss cells, with pronounced effect after 24 h than that of 12 h after Pb and Cr treatment. The SOD activity showed an increasing trend followed by decrease in CAT, POX and GR activity after 12 and 24 h. Both ascorbate and glutathione showed higher accumulation under Pb followed by Cr. The results showed that at high concentration of metals, oxidative stress could be induced in moss cells characterized by the generation of ROS and initiation lipid peroxidation that inhibited the major antioxidant metabolism. Both physiological and ultrastructural studies suggested the possible induction of oxidative stress in Taxithelium nepalense (Schwaegr.) Broth. under Pb and Cr toxicity.

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References

  • Appenroth, K.–J., Stockel, J., Srivastava, A. and Stresser, R. J.: 2001, ‘Multiple effect of chromate on the photosynthetic apparatus of Spirodela polyrhizaprobed by OIJP chlorophyll a fluorescence measurements’, Env. Pollut. 115, 49.

    Article  Google Scholar 

  • Arnon, D. I.: 1949, ‘Copper enzyme in the isolated chloroplast, Polyphenol oxidase in Beta vulgaris’, Plant Physiol. 24, 1.

    Google Scholar 

  • Asada, K. and Takahashi, M.: 1987, ‘Production and scavenging of active oxygen in photosynthetic tissue’, in C. H. Foyer, and P. M. Mullneux (eds), Causes of Photooxidative Stress and Amelioration of Defense System in Plants, CRC Press, Boca Raton, FL.

    Google Scholar 

  • Barcelo, J. and Poschenrieder, C.: 1997, ‘Chromium in plants’, in S. Carati, F. Tottareli and P. Seqmi (eds), Chromium Environmental Issue, Francotangati Press, Milan.

    Google Scholar 

  • Bargagli, R.: 1998, ‘Mosses as passive and active biomonitors of trace elements’, in R. Bargagli (ed.), Trace Elements in Terrestrial Plant, Springer-Verlag, Berlin.

    Google Scholar 

  • Basile, A., Spagnualo, V., Alfano, F. and Castaldo Cobianchi, R.: 1995, ‘Effect of lead and colchicines on morphogenesis in the protenema of the moss Funaria hygrometrica’, Ann. Bot. (London). 76, 597.

    Article  Google Scholar 

  • Bassi, M., Corradi, M. G. and Realini, M.: 1990a, ‘Effect of chromium (VI) on two freshwater plants, Lemna minor and Pistia stratiotes, 1 Morphological observations’, Cytobios. 62, 27.

    Google Scholar 

  • Bassi, M., Corradi, M. G. and Ricci, A.: 1990b, ‘Effect of chromium (VI) on two freshwater plants Lemna minorand Pistia stratiotes, 2 Biochemical and Physiological observations’, Cytobios. 62, 101.

    Google Scholar 

  • Bassi, P., Basile, A., Stefenimi, A., Vosa, C. B., Maffie, M., Giordano, S. and Castaldo Cobianchi, R.: 1995, ‘Effect of lead on the nuclear repetitive DNA of the moss Funaria hygrometrica (Funariales)’, Ann. Bot. (London). 87, 537.

    Article  Google Scholar 

  • Bazynski, T., Krol, M. and Wolinski, D.: 1981, ‘Photosynthetic apparatus of Lemna minor L. as affected by chromate treatment’, in G. Akoyunoglou (ed), Photosynthesis II. Electron Transport and Photophosphorylation, Balaban International Science Service, Philadelphia.

  • Bazzaz, F. A., Rolfe, G. L. and Carlson, R. W.: 1992, ‘Effect of lead on photosynthesis and transpiration of excised leaves of corn and sunflower’, Physiol. Plant. 32, 373.

    Google Scholar 

  • Bowler, C., Van Camp, W. and Inze, D.: 1992, ‘Superoxide dismutase and stress tolerance’, Ann. Rev. Plant Physiol. Mol. Biol. 43, 83.

    Article  Google Scholar 

  • Bowler, C., Van Camp, W., Van Motagu, M. and Inze, D.: 1994, ‘Superoxide dismutase in plants’, Critical Rev. Plant Sci. 13, 119.

    Google Scholar 

  • Brown, D. H. and Sidhu, M.: 1992, ‘Heavy metal uptake, cellular tolerance and inhibition of moss growth’. Cryptogam Bot. 3, 82.

  • Burzynski, M. and Grabowski, A.: 1984, ‘Influence of lead on NO−3 uptake and reduction in cucumber seedlings’, Acta Soc. Bot. Pol. 53, 77.

    Google Scholar 

  • Cabral, J. P.: 2003, ‘ Copper toxicity to five Parmelialichens in vitro’, Env. Exp. Bot. 49, 237.

    Article  Google Scholar 

  • Carballeria, A., Vasquez, M. D. and Lopez, J.: 2001, ‘Biomonitoring of sporadic acidification of rivers on the basis of release of preloaded cadmium from aquatic bryophyte Fantinalis antipyretica. Hedw’, Environ. Pollut. 111, 95.

    Article  PubMed  Google Scholar 

  • Carginale, V., Sorbo, S., Capasso, C., Trinchella, F., Cafiero, G. and Basile, A.: 2004, ‘Accumulation, localization and toxic effects of cadmium in the liverworth Lunularia crucita’, Protoplasma. 223, 53.

    Article  PubMed  Google Scholar 

  • Chance, B. and Maehly, A. C.: 1955, ‘Assay of catalase and peroxidases’, Methods Enzymol. 2, 764.

    Article  Google Scholar 

  • Charles, S. A. and Halliwell, B.: 1981, ‘Light activation of fructose bisphosphate in isolated spinach chloroplast and activation by hydrogen peroxide’, Planta 151, 242.

    Article  Google Scholar 

  • Clemens, C.: 2001, ‘Molecular mechanism of plant metal tolerance and homeostasis’, Planta 212, 475.

    Article  PubMed  Google Scholar 

  • De Fillippis, L. F. and Ziegler, H.: 1993, ‘Effect of sub-lethal concentration of zinc, cadmium and mercury on the photosynthetic carbon reduction cycle of Euglena’, J. Plant Physiol. 142, 167.

    Google Scholar 

  • De Vos, C. H. R. and Schat, H.: 1991, ‘Free radical and heavy metal tolerance’, in J. Rozema and J. A. C. Verkleij (eds), Ecological Responses to Environmental Stresses. Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  • Dietz, K. J., Baier, M. and Kramer, U.: 1999, ‘Free radicals and reactive oxygen species as mediator of heavy metal toxicity in plants’, in M. N. V. Prasad and J. Hagemeyer (eds), Heavy Metal Stress in Plants, From Molecules to Ecosystem. Springer-Verlag, Berlin.

    Google Scholar 

  • Dixit, V., Pandey, V. and Shyam, R.: 2002, ‘ Chromium ion inactivate electron transport and enhance superoxide generation in vivo in pea (Pisum sativumL. cv. Azad) root mitochondria’, Plant, Cell and Env. 25, 687.

    Google Scholar 

  • Elstner, E. F.: 1982, ‘Oxygen activation and oxygen toxicity’, Ann. Rev. Plant Physiol. 33, 73.

    Article  Google Scholar 

  • Elstner, E. F. and Heupel, A.: 1976, ‘Inhibition of nitrate formation from hydroxyl ammonium chloride: A simple assay for superoxide dismutase’, Anal. Biochem. 70, 616.

    Article  PubMed  Google Scholar 

  • Epstein, E., Sagge, O., Cohen, J. D. and Garty, J.: 1986, ‘Endogenous auxin and ethylene in the lichen Ramalina duriaei’, Plant Physiol. 82, 1122.

    Google Scholar 

  • Fridovich, I.: 1995, ‘Superoxide radical and superoxide dismutase’, Ann. Rev. Biochem. 64, 97.

    Article  PubMed  Google Scholar 

  • Gallego, S. M., Benavioes, M. P. and Tomaro, M. L.: 1996, ‘Effect of heavy metal ion excess on sunflower leaves – Evidence for involvement of oxidative stress’, Plant Sci. 121, 151.

    Article  Google Scholar 

  • Garty J., Kloog N., Wolfson R., Cohen Y., Karnieli A. and Avni A.: 1997, ‘The influence of air pollution on the concentration of mineral elements, on spectral reflectance response and on the production of stress ethylene in the lichen Ramalina duriaei’, New Phytol. 137, 587.

    Article  Google Scholar 

  • Garty J., Tamir O., Hassid I., Eshel A., Cohen Y., Karnieli A. and Orlovsky L.: 2001, ‘Photosynthesis, chlorophyll integrity, and spectral reflectance in lichens exposed to air pollution’, J. Env. Qual. 30, 884.

    Google Scholar 

  • Gebicki, J. M. and Bielski, B. H. J.: 1981, ‘Comparison of the capacities of the perhydroxyl and superoxide radicals to initiate chain oxidation of linoleic acid’, J. Am. Chem. Soc. 103, 7020.

    Article  Google Scholar 

  • Giannopolitis, C. N. and Ries, S. K.: 1977, ‘Superoxide dismutase. I. Occurrence in higher plants’, Plant Physiol. 59, 309.

    Google Scholar 

  • Goldbold, D. L. and Kettner, C.: 1991, ‘Lead influences root growth and mineral nutrition of Picea abies seedlings’, J. Plant Physiol. 139, 95.

    Google Scholar 

  • Gonzalez C. M. and Pignata M. I.: 1994, ‘The influence of air pollution on soluble proteins, chlorophyll degradation, MDA, sulfur and heavy metals in a transplanted lichen’, Chem. Ecol. 9, 105.

    Google Scholar 

  • Guo, Y. and Marschner, H.: 1995, ‘Uptake, distribution and binding of cadmium and nickel in different plant species’, J. Plant Nutr. 18, 2691.

    Google Scholar 

  • Halliwell, B. and Gutteridge, J. C. M.: 1988, Free Radicals in Biology and Medicine, Clerodendron Press, Oxford.

    Google Scholar 

  • Heath, R. L. and Packer, K.: 1968, ‘Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation’, Arch. Biochem. Biophys. 125, 189.

    Article  PubMed  Google Scholar 

  • Iqbal, J. and Mustaq, S.: 1987, ‘ Effect of lead on germination, early seedling growth, soluble protein and acid phosphatase content in Zea mays’, Pak. J. Sci. Ind. Res. 30, 853.

    Google Scholar 

  • Kardish N., Ronen R., Bubrick P. and Garty J.: 1987, ‘The influence of air pollution on the concentration of ATP and chlorophyll degradation in lichen Ramalina duriaei (De Not.) Bagl.’, New Phytol. 106, 697.

    Google Scholar 

  • Makela, P., Karkkainen, J. and Somersalo, S.: 2000, ‘Effect of glycine betain on chloroplast ultrastructure, chlorophyll and protein content, and RuBPCO activities in tomato grown under drought or salinity’, Biol. Plant. 43, 471.

    Article  Google Scholar 

  • McGrath, S. P.: 1995, ‘Chromium and Nickel’, in B. J. Alloway (ed), Heavy Metals in Soil, Chapman and Hall, London.

    Google Scholar 

  • Mishra, A. and Choudhuri, M. A.: 1998, ‘Amelioration of lead and mercury effects on germination and rice seedling growth by antioxidants’, Biol. Plant. 41, 469.

    Article  Google Scholar 

  • Moustakas, M., Lanaras, T., Symeonidis, L. and Karataglis, S.: 1994, ‘Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress’, Photosynthetica. 30, 389.

    Google Scholar 

  • Nellesson, H. and Fletcher, J. S.: 1993, ‘Assessment of published literature on the uptake, accumulation and translocation of heavy metals in vascular plants’, Chemosphere. 9, 1669.

    Article  Google Scholar 

  • Nieboer, E. and Richardson, D. H. S.: 1980, ‘The replacement of nondescript term ≪heavy metals≫ by a biologically and chemically significant classification of metal ions’, Environ. Pollut. Ser. 1, 3.

    Article  Google Scholar 

  • Oser, B. L.: 1979, Hawks Physiological Chemistry, McGraw-Hill, New York.

    Google Scholar 

  • Panda, S. K.: 2003, ‘Heavy metal phytotoxicity induces oxidative stress in a moss, Taxithellium sp.’, Curr. Sci. 84(5), 631.

  • Panda, S. K. and Khan, M. H.: 2003, ‘Chromium phytotoxicity effects on the germination of green gram (Vigna radiata) seeds’, J. Phytol. Res. 15, 225.

    Google Scholar 

  • Panda, S. K. and Patra, H. K.: 1997, ‘Physiology of chromium toxicity. A review’, Plant Physiol. Biochem. 24, 10.

    Google Scholar 

  • Panda, S. K. and Patra, H. K.: 2000, ‘Does chromium (III) produces oxidative damage in excised wheat leaves?’ J. Plant Biol. 27, 105.

    Google Scholar 

  • Panda, S. K., Chaudhury, I. and Khan, M. H.: 2003a, ‘Heavy metal induced lipid peroxidation and affect antioxidants in wheat leaves’, Biol. Plant. 46(2), 289.

    Article  Google Scholar 

  • Panda, S. K., Singha, L. B. and Khan, M. H.: 2003b, ‘Does aluminum phytotoxicity induces oxidative stress in green gram (Vigna radiata)?’ Bulg. J. Plant Physiol. 29, 77.

    Google Scholar 

  • Patra, J. B., Panda, B.: 1998. ‘A comparison of biochemical responses to oxidative and metal stress of barley (Hordeum vulgare L.)’. Environ. Pollut. 101, 99.

    Article  PubMed  Google Scholar 

  • Poskuta, J. W., Parys, E. and Ramanowska, E.: 1987, ‘The effects of lead on the gaseous exchange and photosynthetic carbon metabolism of pea seedlings’, Acta Soc. Bot. Pol. 56, 127.

    Google Scholar 

  • Prasad, K. V. S. K., Pardha Saradhi, P. and Shramila, P.: 1999, ‘Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea’, Env. Exp. Bot. 42, 1.

    Article  Google Scholar 

  • Rao, D. N., Robitaille, G. and Leblance, F.: 1977, ‘Influence of heavy metal pollution on lichens and bryophytes’, J. Hattori. Bot. Lab. 42, 213.

    Google Scholar 

  • Rennenberg, H.: 1982, ‘Glutathione metabolism and possible role in higher plants’, Phytochemistry 28, 2771.

    Google Scholar 

  • Sagisaka, S.: 1979, ‘The occurrence of peroxide in perennial plant Populus glerica’, Plant Physiol. 57, 308.

    Google Scholar 

  • Salt, D. E., Smith, R. D. and Raskin, I.: 1998, ‘Phytoremadation’, Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 643.

    Article  PubMed  Google Scholar 

  • Sandalio, L. M., Dalurzo, H. C., Gomez, M., Romero-Puertas, M. C. and del Rio, L. A.: 2001, ‘Cadmium induced changes in growth and oxidative metabolism of pea plants’, J. Exp. Bot. 52, 2115.

    PubMed  Google Scholar 

  • Saxena, D. K., Saxena, A. and Srivastava, H. S.: 1999, ‘Heavy metal accumulation and in vivo nitrate reductase activity in the Sphagnum squarrosum Carm. Samnl.’, Proc. Nat. Acad. Sci. India. 69, 307.

    Google Scholar 

  • Saxena, D. K., Sharma, B. K., Srivastava, H. S., Saxena, A., Sankar, N., Kamakshi and Goswami, A.: 2001, ‘Need of bryophyte bank for environment monitoring in India’, Curr. Sci. 81, 1395.

    Google Scholar 

  • Smith, I. K., Vierhgeller, T. L. and Throne, C. A.: 1988, ‘Assay of glutathione reductase in crude tissue homogenate using 5, 5′, dithiobis (2-nitrobenzoic acid)’, Anal. Biochem. 175, 408.

    Article  PubMed  Google Scholar 

  • Somashekaraiah, B. V., Padmaja, K. and Prasda, A. R. K.: 1992, ‘Phytotoxicity of cadmium ions on germinating seedlings of mung beans (Phaseolus vulgaris). Involvement of lipid peroxides in chlorophyll degradation’, Physiol. Plant. 85, 85.

    Article  Google Scholar 

  • Van Assche, F. and Clijsters, H.: 1990, ‘Effect of metals on enzyme activity in plants’, Plant Cell Environ. 13, 195.

    Google Scholar 

  • Verma, S. and Dubey, R. S.: 2003, ‘Lead toxicity indices lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants’, Plant Sci. 164(4), 645.

    Article  Google Scholar 

  • Vincent, C. D., Lawlor, A. J. and Tipping, E.: 2001, ‘Accumulation of Al, Mn, Fe, Cu, Zn, Cd and Pb by the bryophyte Scapania undulate in three upland waters of different pH’, Ecotoxicol. Environ. Saf. 44, 12.

    Google Scholar 

  • Vranova, E., Inze, D. and Van Breusegem, F.: 2002, ‘Signal transduction during oxidative stress’, J. Exp. Bot. 53(372), 1227.

    Article  Google Scholar 

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  1. Plant Biochemistry Laboratory, School of Life Sciences, Assam (Central) University, Silchar, 788 011, India

    Shuvasish Choudhury & Sanjib Kumar Panda

  2. Research Institute for Bioresources, Okayama University, Kurashiki, 7100 046, Japan

    Sanjib Kumar Panda

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  1. Shuvasish Choudhury
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Choudhury, S., Panda, S.K. Toxic Effects, Oxidative Stress and Ultrastructural Changes in Moss Taxithelium Nepalense (Schwaegr.) Broth. Under Chromium and Lead Phytotoxicity. Water Air Soil Pollut 167, 73–90 (2005). https://doi.org/10.1007/s11270-005-8682-9

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