Abstract
The Patnaik histological grade is a good method for the prediction of long-term mast cell tumour behaviour but it is influenced by subjective inter-observer variations and intratumoral heterogeneity. The present study evaluated each of the histopathological parameters used to formulate Patnaik’s grade in terms of prognosis and tested whether they have a different prognostic sensitivity, thereby disclosing which could be considered more useful in the prediction of tumour recurrence and patient survival. Clinical presentation (single or multiple tumours) was also considered as possible prognostic factor. The results demonstrated that individual histological criteria together with multiple presentation may be of value in predicting the outcome of mast cell tumours. Among these, invasiveness (β1.85; standard error 1.15) and the number of mitotic figures (β3.01; standard error 1.18) showed high prognostic significance (Cox proportional hazard regression for censored data ; chi-squared = 15.52, degree of freedom = 6, p = 0.016) and could serve as reliable prognostic indicators avoiding more subjective parameters such as cellular differentiation, nuclear morphology and tumoural pattern.
Similar content being viewed by others
Abbreviations
- hpf:
-
high-power field
- MCT:
-
mast cell tumour
- PFI:
-
progress in-free interval
- ST:
-
survival time
References
Abadie, J.J., Amardeilh, M.A. and Deverdier, M.E., 1999. Immunohistochemical detection of proliferating cell nuclear antigen and Ki67 in mast cell tumors from dogs. Journal of the American Veterinary Medical Association, 215, 1629–1634
Al-Sarraf, R., Maudlin, G.N., Patnaik, A.K. and Meleo, K.A., 1996. A prospective study of radiation therapy for the treatment of grade 2 mast cell tumors in 32 dogs. Journal of Veterinary Internal Medicine, 10, 376–378
Bostock, D.E., 1973. The prognosis following surgical removal of mastocytomas in dogs. Journal of Small Animal Practice, 14, 27–41
Bostock, D.E., Crocker, J., Harris, K. and Smith, P., 1989. Nucleolar organiser regions as indicators of postsurgical prognosis in canine spontaneous mast cell tumours. British Journal of Cancer, 59, 915–918
Camussi, A., Möller F., Ottaviano, E. and Sari Gorla M., 1991. Metodi Statistici per la Sperimentazione Biologica, 4th edn., (Zanichelli, Bologna)
Macy, D.W., 1984. Canine and feline mast cell tumours: biologic behavior, diagnosis and therapy. Seminars in Veterinary Medicine and Surgery, 1, 72–83
Michels, G.M., Knapp, D.W., DeNicola, D.B., Glickmann, N. and Bonney, P., 2002. Prognosis following surgical excision of canine cutaneous mast cell tumors with histopathologically tumor free versus nontumor free margins: a retrospective study of 31 cases. Journal of the American Animal Hospital Association, 38, 458–466
Misdorp, W., 1987. Incomplete surgery, local immunostimulation and recurrence of some tumour types in dogs and cats. Veterinary Quarterly, 9, 279–286
Murphy, S., Sparkes, A.H., Smith, K.C., Blunden, A.S. and Brearly, M.J., 2004. Relationships between the histological grade of cutaneous mast cell tumours in dogs, their survival and the efficacy of surgical resection. The Veterinary Record, 154, 743–746
Northrup, N.C., Harmon, B.G., Gieger, T.L., Brown, C.A., Carmichael, K.P., Garcia, A., Latimer, K.S., Munday, J.S., Rakich, P.M., Richey, L.J., Stedman, N.L., Cheng, A.L. and Howerth, E.W., 2005. Variation among pathologists in histologic grading of canine cutaneous mast cell tumours. Journal of Veterinary Diagnostic Investigation, 17, 245–248
O'Keefe, D.A., 1990. Canine mast cell tumors. Veterinary Clinics of North America, Small Animal Practice, 20, 1105–1115
Patnaik, A.K., Ehler, W.Y. and MacEwen, E.G., 1984. Canine cutaneous mast cell tumors: morphologic grading and survival time in 83 dogs. Veterinary Pathology, 21, 469–474
Preziosi, R., Sarli, G. and Paltrinieri, M., 2004. Prognostic value of intratumoral vessel density in cutaneous mast cell tumours of the dog. Journal of Comparative Pathology, 130, 143–151
Rogers, K.S., 1996. Mast cell tumours: dilemmas of diagnosis and treatment. Veterinary Clinics of North America: Small Animal Practice, 26, 87–101
Séguin, B., Leibman, N.F., Bregazzi, S.V., Ogilvie, G.K., Powers, B.E., Dernell, W.S., Fettmann, M.J. and Withrow, S.J., 2001. Clinical outcome of dogs with grade-II mast cell tumors treated with surgery alone: 55 cases (1996–1999). Journal of the American Veterinary Medical Association, 218, 1120–1123
Simoes, J.P.C., Schoning, P. and Butine, M., 1994. Prognosis of canine mast cell tumors: a comparison of three methods. Veterinary Pathology, 31, 637–647
Strefezzi, R.De F., Xavier, J.G. and Catão-Dias, J.L., 2003. Morphometry of canine cutaneous mast cell tumors. Veterinary Pathology, 40, 268–275
Vail, D.M., 1996. Mast cell tumors. In: S.J. Withrow and E.G. MacEwen (eds), Small Animal Clinical Oncology, (WB Saunders, Philadelphia), 192–210
Walder, E.J. and Gross, T.L., 1992. Canine mast cell tumors. In: T.L. Gross, P.J, Ihrke and E.J. Walder (eds), Veterinary Dermatopathology – a Macroscopic and Microscopic Evaluation of Canine and Feline Skin Disease, (Mosby Year Book, St Louis), 470–473
Author information
Authors and Affiliations
Corresponding author
Additional information
R. Preziosi Deceased
Rights and permissions
About this article
Cite this article
Preziosi, R., Sarli, G. & Paltrinieri, M. Multivariate Survival Analysis of Histological Parameters and Clinical Presentation in Canine Cutaneous Mast Cell Tumours. Vet Res Commun 31, 287–296 (2007). https://doi.org/10.1007/s11259-006-3427-9
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11259-006-3427-9