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Prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of Mexico

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Abstract

A region-wide survey was conducted in the tropical area of Tierra Caliente, State of Guerrero, Mexico to estimate the prevalence of subclinical bovine mastitis (SCM), distribution of mastitis pathogens, and in vitro antimicrobial susceptibility of different mastitis pathogens in dairy farms. In total, 1036 quarter milk samples were obtained from 259 cows at 87 different dairy farms. Collected quarter milk samples were submitted for California Mastitis Test (CMT), bacteriological examination, and testing for antimicrobial susceptibility. Overall prevalence of SCM in the studied area was 20.5 %. Prevalence in the different regions was as follows: 28 % in Arcelia municipality, 21 % in Tlalchapa municipality, 19.4 % in Pungarabato municipality, and 14.3 % in Finch Cutzamala municipality. Of all positive isolates, 97.5 % were Gram-negative bacteria. Moreover, of all positive isolates, 37.5 % were Proteus vulgaris, 25 % Salmonella spp., 12.5 % Enterobacter aerogenes, and 10 % Escherichia coli. Klebsiella pneumonia and E. coli were sensitive for netilmicin antimicrobial. However, E. coli was sensitive for pefloxacin and gentamicin with a sensitivity for pefloxacin for E. aerogenes, while Staphylococci were sensitive for gentamicin and dicloxacillin. It could be concluded that practices such as the implementation of mastitis control programs, improved milking hygiene together with an intramammary treatment with netilmicin, pefloxacin, and gentamicin antimicrobials should be considered for mastitis prevention in the study area of Tierra Caliente, in the tropical area of Guerrero, Mexico.

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Abbreviations

CMT:

California Mastitis Test

I:

Intermediate resistance

R:

Resistant

S:

Sensitive

SCM:

Subclinical bovine mastitis

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Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the official Mexican standard of animals care (NOM-051-ZOO-1995).

Conflict of interest

All authors declare that there are no present or potential conflicts of interest among the authors and other people or organizations that could inappropriately bias their work.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Author information

Authors and Affiliations

  1. Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Altamirano, P.O. 40660, Mexico

    Jaime Olivares-Pérez, Saul Rojas-Hernández, David Velázquez-Reynoso, Moisés Cipriano-Salazar & Luis Miguel Camacho-Díaz

  2. Dairy Science Department, National Research Centre, 33 Bohouth St. Dokki, Giza, Egypt

    Ahmed Eid Kholif

  3. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, Mexico

    Mona Mohamed Mohamed Yasseen Elghandour, Abdelfattah Zeidan Mohamed Salem & María Uxúa Alonso-Fresán

  4. Universidad Autónoma del Estado de Hidalgo, Instituto de Ciencias Agropecuarias, Rancho Universitario, Av. Universidad Km. 1, Ex-Hda. de Aquetzalpa AP 32, CP 43600, Tulancingo, Hidalgo, Mexico

    Adrian Zaragoza Bastida

  5. North Florida Research and Education Center, Department of Animal Sciences, University of Florida, Marianna, FL, USA

    Nicolas DiLorenzo

Authors
  1. Jaime Olivares-Pérez
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  2. Ahmed Eid Kholif
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  3. Saul Rojas-Hernández
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  4. Mona Mohamed Mohamed Yasseen Elghandour
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  5. Abdelfattah Zeidan Mohamed Salem
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  6. Adrian Zaragoza Bastida
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  7. David Velázquez-Reynoso
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  8. Moisés Cipriano-Salazar
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  9. Luis Miguel Camacho-Díaz
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  10. María Uxúa Alonso-Fresán
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  11. Nicolas DiLorenzo
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Corresponding author

Correspondence to Abdelfattah Zeidan Mohamed Salem.

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Olivares-Pérez, J., Kholif, A.E., Rojas-Hernández, S. et al. Prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of Mexico. Trop Anim Health Prod 47, 1497–1504 (2015). https://doi.org/10.1007/s11250-015-0890-8

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  • DOI: https://doi.org/10.1007/s11250-015-0890-8

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