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First serological and molecular evidence of PPRV occurrence in Ghardaïa district, center of Algeria

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Abstract

In February 2012, an outbreak of peste des petits ruminants (PPR) was suspected in Ghardaïa district at the center of Algeria. Clinical, serological, and molecular investigations were performed to confirm the occurrence of PPRV. The overall morbidity, mortality, and case fatality rates of the ten flocks investigated were 12.2, 2.5, and 20.3 %, respectively. At the flock level, positivity to PPR was 100, 90, and 100 % by competitive ELISA (c-ELISA), RT-PCR of blood samples, and oculo-nasal swabs, respectively. At the individual levels, the present study showed that out of 186 samples collected from the same animals 17/62 (27.41 %), 14/62 (22.85 %), and 36/62 (58.06 %) were positive by c-ELISA, RT-PCR of blood samples, and RT PCR of oculo-nasal swabs, respectively. The positivity of PPR was significantly higher using RT-PCR of oculo-nasal swabs than c-ELISA and RT-PCR of blood samples. The N gene partial sequence of five PPRV-positive amplicons revealed 100 % homology among them and phylogenetically belonged to lineage IV. The sequences also showed similarity range of 97–99 % with the strains implicated in the Moroccan and Tunisian outbreaks, however, suggesting that a similar strain is circulating across this area of the Maghreb and highlighting the need for a regional control approach.

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Acknowledgments

Special thanks to the FAO/OIE reference laboratory of PPR (CIRAD, Montpelier, France) for the sequencing.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Institut National de la Médecine Vétérinaire (INMV), BP 205, Hacen Badi, El Harrach, Algiers, Algeria

    Moustafa Kardjadj

  2. Laboratoire de Recherche “Santé et Productions Animales”, Ecole Nationale Supérieure Vétérinaire d’Alger, El Alia, Algiers, Algeria

    Moustafa Kardjadj & Meriem-Hind Ben-Mahdi

  3. Applied Biotechnology Division, National Veterinary Research Institute, Vom, Plateau, Nigeria

    Pam Dachung Luka

Authors
  1. Moustafa Kardjadj
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  2. Meriem-Hind Ben-Mahdi
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  3. Pam Dachung Luka
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Correspondence to Moustafa Kardjadj.

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Kardjadj, M., Ben-Mahdi, MH. & Luka, P.D. First serological and molecular evidence of PPRV occurrence in Ghardaïa district, center of Algeria. Trop Anim Health Prod 47, 1279–1284 (2015). https://doi.org/10.1007/s11250-015-0860-1

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  • DOI: https://doi.org/10.1007/s11250-015-0860-1

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