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Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1

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Abstract

Potato virus Y (PVY) is the most important viral pathogen of cultivated potato (Solanum tuberosum) from a commercial perspective, causing severe losses in both tuber quality and yield worldwide. Specific accessions of wild potato species exhibit resistance against PVY but efforts to transfer the trait to cultivated material have not yielded widely adopted varieties. Because amino acid substitutions at specific domains of host factor eIF4E-1 often confer resistance to various crops, we sequenced the associated genes expressed in wild potato plants. A novel eIF4E-1 variant, designated here as Eva1, was identified in S. chacoense, S. demissum, and S. etuberosum. The protein contains amino acid substitutions at ten different positions when compared to its cultivated potato (S. tuberosum) homolog. In the yeast two-hybrid system, Eva1 failed to bind VPg, a viral protein required for infectivity. Overexpression of the associated cDNA conferred PVY resistance to transgenic potato plants silenced for the native eIF4E-1 gene. Because the gene sources of Eva1 are sexually compatible with potato, the molecular strategies described can be employed to develop ‘intragenic’ potato cultivars.

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Acknowledgments

We thank Dr. Hua Yan, Heather Holloway, Matthew Burns, and Christie Caplinger for excellent technical support.

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Authors and Affiliations

  1. Simplot Plant Sciences, JR Simplot Company, Boise, ID, 83706, USA

    Hui Duan, Craig Richael & Caius M. Rommens

Authors
  1. Hui Duan
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  2. Craig Richael
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  3. Caius M. Rommens
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Corresponding author

Correspondence to Caius M. Rommens.

Electronic supplementary material

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11248_2011_9576_MOESM1_ESM.ppt

Supplementary Figure 1. Symptom development following PVYNTN infection. Leaves shown are from a mock-infected control plant (left) and a plant that had been infected two weeks before (right). (PPT 1560 kb)

11248_2011_9576_MOESM2_ESM.ppt

Supplementary Figure 2. SteIF4E-1 transcript levels in silencing transgenic potato. Leaf RNA from the wild type Russet Burbank (wt), transgenic plants carrying the empty vector control (vc), and plants from the transgenic lines 1895/1-13 were hybridized with a SteIF4E-1-derived probe. Ethidium bromide-stained ribosomal RNA was used as a loading control. (PPT 563 kb)

11248_2011_9576_MOESM3_ESM.docx

Supplementary Figure 3. Alignment of resistance-associate eIF4E proteins. Amino acid differences with the homologous proteins of susceptible genotypes are highlighted in gray. (DOCX 18 kb)

Supplementary material 4 (PDF 32 kb)

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Duan, H., Richael, C. & Rommens, C.M. Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1 . Transgenic Res 21, 929–938 (2012). https://doi.org/10.1007/s11248-011-9576-9

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  • DOI: https://doi.org/10.1007/s11248-011-9576-9

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