Skip to main content
SpringerLink
Log in

An efficient and quick protocol for in vitro multiplication of snake plant, Sansevieria trifasciata var. Laurentii [Prain]

  • Protocol Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments sized 1 cm were surface sterilized and inoculated on Murashige and Skoog media supplemented with 3% sucrose, 0.8% agar, and various concentrations of indole-3-butyric acid (1 to 10 mg/L). Cultures were maintained for 4 to 5 weeks at standard conditions to allow root induction and elongation. Shoot induction was triggered upon altering the daytime culture room temperature to 37 ± 2 °C. Multiple shoots were produced at higher IBA concentrations. Another 5 weeks later, individual plantlets were excised and hardened for 2 weeks in soil preparation contained in small cups before transferring to 30 × 20 × 20 cm sized pots. We discuss the probable events effectuating unusual shoot regeneration at relatively higher temperatures in media without supplementing any cytokinins.

Key message

This manuscript offers a unique protocol for rapid in vitro regeneration in snake plants using leaf explants cultured in airtight-sealed vessels. The success of the protocol relies on IBA led root induction at standard tissue culture temperatures, while shoot induction uses only an abrupt daytime shift to relatively high temperatures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anbu JS, Jayaraj P, Varatharajan R, Thomas JG, Jisha J, Muthappan M (2009) Analgesic and antipyretic effects of Sansevieria trifasciata leaves. Afr J Tradit Complement Altern Med: AJTCAM 6:529–533

    PubMed  PubMed Central  Google Scholar 

  • Andhare RN, Raut MK, Naik SR (2012) Evaluation of antiallergic and anti-anaphylactic activity of ethanolic extract of Sanseveiria trifasciata leaves (EEST) in rodents. J Ethnopharmacol 142:627–633. https://doi.org/10.1016/j.jep.2012.05.007

    Article  CAS  PubMed  Google Scholar 

  • Biddington NL (1992) The influence of ethylene in plant tissue culture. Plant Growth Regul 11:173–187. https://doi.org/10.1007/BF00024072

    Article  CAS  Google Scholar 

  • Biddington N, Robinson HT (1990) Variations in response to high temperature treatments in anther culture of Brussels sprouts. Plant Cell Tissue Organ Cult 22:48–54

    Google Scholar 

  • Biddington N, Robinson HT (1991) Ethylene production during anther culture of Brussels sprouts (Brassica oleracea var gemmifera) and its relationship with factors that affect embryo production Plant Cell. Tissue Organ Cult 25:169–177

    CAS  Google Scholar 

  • Biondi S, Diaz T, Iglesias I, Gamberini G, Bagni N (1990) Polyamines and ethylene in relation to adventitious root formation in Prunus avium shoot cultures. Physiol Plant 78:474–483

    Article  Google Scholar 

  • Boraphech P, Thiravetyan P (2015) Removal of trimethylamine (fishy odor) by C3 and CAM plants. Environ Sci Pollut Res 22:11543–11557. https://doi.org/10.1007/s11356-015-4364-3

    Article  CAS  Google Scholar 

  • Cheng T-S, Floros J, Shewfelt R, Chang C (1988) The effect of high-temperature stress on ripening of tomatoes (Lycopersicon esculentum). J Plant Physiol 132:459–464

    Article  CAS  Google Scholar 

  • Cornejo-Martin M, Mingo-Castel A, Primo-Millo E (1979) Organ redifferentiation in rice callus: effects of C2H4, CO2 and cytokinins. Zeitschrift für Pflanzenphysiologie 94:117–123

    Article  CAS  Google Scholar 

  • Dalton C, Street H (1976) The role of the gas phase in the greening and growth of illuminated cell suspension cultures of spinach (Spinacia oleracea, L.). In Vitro 12:485–494

    Article  CAS  Google Scholar 

  • De Pereira-Netto AB, McCown BH (1999) Thermally induced changes in shoot morphology of Hancornia speciosa microcultures: evidence of mediation by ethylene. Tree Physiol 19:733–740. https://doi.org/10.1093/treephys/19.11.733

    Article  PubMed  Google Scholar 

  • George EF (1996) Plant propagation by tissue culture. Exegetics Ltd., Wilts, England

    Google Scholar 

  • Hessayon DG (1996) The house plant expert. pbi Publications, Brittanica House, Waltham Cross, HertsEngland

  • Huxter TJ, Thorpe TA, Reid DM (1981) Shoot initiation in light-and dark-grown tobacco callus: the role of ethylene. Physiol Plant 53:319–326

    Article  CAS  Google Scholar 

  • Irga PJ, Pettit TJ, Torpy FR (2018) The phytoremediation of indoor air pollution: a review on the technology development from the potted plant through to functional green wall biofilters. Rev Environ Sci Bio/technol 17:395–415. https://doi.org/10.1007/s11157-018-9465-2

    Article  CAS  Google Scholar 

  • Joyner JF, Gangstad EO, Seale CC (1951) The vegetative propagation of sansevieria1. Agronomy J 43:128–130

    Article  Google Scholar 

  • Kigel J, Konsens I, Ofir M (1991) Branching, flowering and pod-set patterns in snap-bean (Phaseolus vulgaris L.) as affected by temperature. Canad J Plant Sci 71:1233–1242

    Article  Google Scholar 

  • Kim KJ, Lee DW (2008) Efficiency of volatile formaldehyde removal of orchids as affected by species and crassulacean acid metabolism (CAM) nature. Horticul Environ Biotechnol 49:132–137

    CAS  Google Scholar 

  • Kudoyarova GR et al (2014) Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP). J Exp Bot 65:2287–2294. https://doi.org/10.1093/jxb/eru113

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kumar PP, Reid DM, Thorpe TA (1987) The role of ethylene and carbon dioxide in differentiation of shoot buds in excised cotyledons of Pinus radiata in vitro. Physiol Plant 69:244–252

    Article  CAS  Google Scholar 

  • Kumar PP, Rao CD, Goh C-J (1996) Ethylene and CO2 affect direct shoot regeneration from the petiolar ends of Paulownia kawakamii leaves cultured in vitro. Plant Growth Regul 20:237–243. https://doi.org/10.1007/BF00043313

    Article  CAS  Google Scholar 

  • Le Guyader H (1987) Ethylène et différenciation de racines par des cultures de couches cellulaires minces de tabac Comptes rendus de l’Académie des sciences Série 3. Sciences De La Vie 305:591–594

    Google Scholar 

  • Lefebvre R (1972) Compared effects of an anaerobic treatment and ethylene on the budding and synthesis of foliar pigments. [Chicoky] Acad Sci Paris CR Ser D 193–195

  • Mimaki Y, Inoue T, Kuroda M, Sashida Y (1996) Steroidal saponins from Sansevieria trifasciata. Phytochemistry 43:1325–1331

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15: 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

  • Nogué F, Gonneau M, Faure J-D (2003) Cytokinins. In: Henry HL, Norman AW (eds) Encyclopedia of Hormones. Academic Press, New York, pp 371–378. https://doi.org/10.1016/B0-12-341103-3/00061-9

  • Nour KA, Thorpe TA (1994) The effect of the gaseous state on bud induction and shoot multiplication in vitro in eastern white cedar. Physiol Plant 90:163–172. https://doi.org/10.1111/j.1399-3054.1994.tb02207.x

    Article  CAS  Google Scholar 

  • Ramanaiah K, Prasad AR, Reddy KHC (2013) Mechanical, thermophysical and fire properties of sansevieria fiber-reinforced polyester composites. Mater Design 49:986–991

    Article  CAS  Google Scholar 

  • Riov J, Yang SF (1989) Ethylene and auxin-ethylene interaction in adventitious root formation in mung bean (Vigna radiata) cuttings. J Plant Growth Regul 8:131. https://doi.org/10.1007/BF02025280

    Article  CAS  Google Scholar 

  • Sarmast M, Salehi M, Salehi H (2009) The potential of different parts of Sansevieria trifasciata L. leaf for meristemoids production. Aust J Basic Appl Sci 3:2506–2509

    CAS  Google Scholar 

  • Sarmast MK, Salehi H, Khosh-Khui M (2014) Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants. 3 Biotech 4:599–604. https://doi.org/10.1007/s13205-013-0191-8

    Article  PubMed  Google Scholar 

  • Schoellhorn RK, Barrett JE, Nell TA (1996) Branching of chrysanthemum cultivars varies with season, temperature, and photosynthetic photon flux. HortScience 31:74–78

    Article  Google Scholar 

  • Shekhawat N, Rathore T, Singh R, Deora N, Rao SR (1993) Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Regul 12:273–280

    Article  CAS  Google Scholar 

  • Sreenivasan V, Somasundaram S, Ravindran D, Manikandan V, Narayanasamy R (2011) Microstructural, physico-chemical and mechanical characterisation of Sansevieria cylindrica fibres–An exploratory investigation. Mater Design 32:453–461

    Article  CAS  Google Scholar 

  • Sriprapat W, Suksabye P, Areephak S, Klantup P, Waraha A, Sawattan A, Thiravetyan P (2014) Uptake of toluene and ethylbenzene by plants: removal of volatile indoor air contaminants. Ecotoxicol Environ Saf 102:147–151

    Article  CAS  Google Scholar 

  • Takawira R, Nordal I (2001) The genus Sansevieria (family Dracaenaceae) in Zimbabwe. In: XX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals-Part II, 572:189–198

  • Tchegnitegni BT, Teponno RB, Jenett-Siems K, Melzig MF, Miyamoto T, Tapondjou LA (2017) A dihydrochalcone derivative and further steroidal saponins from Sansevieria trifasciata Prain. Z Naturforsch [c] 72:477–482. https://doi.org/10.1515/znc-2017-0027

    Article  CAS  Google Scholar 

  • Teponno RB, Tanaka C, Jie B, Tapondjou LA, Miyamoto T (2016) Trifasciatosides A-J steroidal saponins from Sansevieria Trifasciata. Chem Pharmac Bull 64:1347–1355. https://doi.org/10.1248/cpb.c16-00337

    Article  CAS  Google Scholar 

  • Thomas DdS, Murashige T (1979) Volatile emissions of plant tissue cultures. I. Identification of the major components. Vitro 15:654–658

    Article  CAS  Google Scholar 

  • Torres KC (1989) In vitro propagation of snake plant (Sansevieria). In: Tissue culture techniques for horticultural crops. Springer, pp 92–95. https://doi.org/10.1007/978-1-4615-9756-8_8

  • Wolverton BC, Johnson A, Bounds K (1989) Interior landscape plants for indoor air pollution abatement. NTRS - NASA Technical Reports Server, work supported by the NASA Office of Commercial Programs-Technology Utilization Division, and the Associated Landscape Contractors of America (ALCA) National Aeronautics and Space Administration, John C Stennis Space Center, Science and Technology Laboratory, Stennis Space Center, MS 39529–6000 1/07/1989:27

  • Yu Y-B, Adams DO, Yang SF (1980) Inhibition of ethylene production by 2, 4-dinitrophenol and high temperature. Plant Physiol 66:286–290

    Article  CAS  Google Scholar 

  • Yuningsih LM, Batubara I, Darusman LK (2014) Sansevieria trifasciata Properties as Lead (II) Ion Biosorbent. Makara J Sci. https://doi.org/10.7454/mss.v18i2.3139

    Article  Google Scholar 

  • Yusnita Y, Pungkastiani W, Hapsoro D (2011) In vitro organogenesis of two Sansevieria trifasciata cultivars on different concentrations of benzyladenine (BA). AGRIVITA, J Agricul Sci 33:147–153. https://doi.org/10.17503/agrivita.v33i2.57

    Article  Google Scholar 

Download references

Acknowledgements

Authors wish to thank University Center for Research and Development (UCRD) and University Institute of Biotechnology (UIBT) for infrastructural support. Authors do not have any conflict of interests.

Author information

Authors and Affiliations

  1. Chandigarh University, UIBT, Gharuan, Mohali, Punjab, 140413, India

    Jaspreet Kaur & Gaurav Mudgal

Authors
  1. Jaspreet Kaur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gaurav Mudgal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gaurav Mudgal.

Additional information

Communicated by Maurizio Lambardi.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (PDF 289 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaur, J., Mudgal, G. An efficient and quick protocol for in vitro multiplication of snake plant, Sansevieria trifasciata var. Laurentii [Prain]. Plant Cell Tiss Organ Cult 147, 405–411 (2021). https://doi.org/10.1007/s11240-021-02132-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-021-02132-0

Keywords

Search

Navigation