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An efficient method for inducing multiple genotypes of tetraploids Lilium rosthornii Diels

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Abstract

Polyploids generally show strong environmental adaptation and diverse morphological variations. Therefore, polyploid induction is an important protocol for plant breeding. Lilium rosthornii is a wild lily species with high horticultural value and excellent disease resistance. In this study, seeds of L. rosthornii were subjected to polyploidy induction treatments to obtain multiple genotypes of tetraploids. Germinated seeds were immersed in two antimitotic agents at different concentrations for various times. In total, 199 tetraploid genotypes were obtained. The most efficient treatments of each agent were immersed in 0.05% colchicine for 36 h and in 0.01% oryzalin for 24 h; the induction rate of the former (27.78%) was significantly higher than that of the latter (22.22%). The swollen hypocotyl phenotype after colchicine and oryzalin treatments was strongly correlated with tetraploidy (0.989** and 0.975**, respectively), suggesting that this phenotype could serve as an early ploidy selection trait. The correlations were weaker between stomata length/density and tetraploidy (0.773** and 0.695**, respectively), implying that stomatal characters are affected by both the ploidy level and genotype. After several rounds subculture in vitro, the morphology and growth traits were not significantly different between diploids and tetraploids, but there were wider variations in these parameters in tetraploids than in diploids. After transplanting, the bulblet germination rate was higher in tetraploids than in diploids. The leaf phenotype did not differ between tetraploids and diploids initially, but the leaves of tetraploids became larger than those of diploids over time. Together, these suggest that tetraploids, may contribute diverse characteristics to lily breeding.

Key message

Multiple genotypes of tetraploid Lilium rosthornii were induced by colchicine and oryzalin. Swollen hypocotyl was associated with polyploidy. Compared with diploids, tetraploids showed wider variations in morphological parameters.

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Abbreviations

ANOVA:

One-way analysis of variance

BA:

Benzylaminopurine

FCM:

Flow cytometry

MS:

Murashige–Skoog

NAA:

Naphthylacetic

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Acknowledgements

This work was supported by Grants from the National Key R & D Program of China (2019YFD1001002) and Funds for the National Natural Science Foundation of China (Grant No. 31772348).

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Authors and Affiliations

  1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plant of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China

    Lian-Juan Wang, Qian Zhang, Xue Gao & Gui-Xia Jia

  2. School of Agroforestry & Medicine, The Open University of China, Beijing, 100039, China

    Qin-Zheng Cao

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  1. Lian-Juan Wang
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  2. Qian Zhang
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  3. Qin-Zheng Cao
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  4. Xue Gao
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  5. Gui-Xia Jia
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Contributions

G-XJ and L-JW conceived and designed the experiments; L-JW, QZ and XG performed the experiments; L-JW analyzed the data and wrote the manuscript; G-XJ, L-JW and Q-ZC revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Gui-Xia Jia.

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Communicated by M. I. Beruto.

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Wang, LJ., Zhang, Q., Cao, QZ. et al. An efficient method for inducing multiple genotypes of tetraploids Lilium rosthornii Diels. Plant Cell Tiss Organ Cult 141, 499–510 (2020). https://doi.org/10.1007/s11240-020-01807-4

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