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Regeneration of daylily (Hemerocallis) from young leaf segments

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Abstract

Calli were initiated from leaf segments (~0.5 × 0.5 cm) of daylily incubated on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-dichlorophenoxyacetic acid (2.4-D), and either benzyladenine (BA) or thidiazuron (TDZ). The highest frequency of callus induction was observed on medium with 6.79 μM 2,4-D plus either 4.55 or 6.81 μm TDZ. A period of callus maintenance on medium containing 5.37 μM naphthaleneacetic acid (NAA) plus 2.22 or 4.44 μM BA was necessary following induction to improve the quality of the callus, and significantly increase the frequency of embryogenic-like callus formation and shoot regeneration once calli were transferred to light. Over 70% of the regenerated shoots produced roots on ½ strength MS medium lacking plant growth regulators. The regenerated plantlets were successfully transferred into soil and acclimatized in growth rooms. This is the first report showing that leaf segments can be used for daylily regeneration.

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Acknowledgments

We wish to thank Linda Pinkham for providing the daylily seeds used in these experiments. Also we wish thank Dr. Barry Flinn’s encouraging the study of daylily. This project was funded by the United States Department of Agriculture, as well as through operating funds provided by the Commonwealth of Virginia.

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Authors and Affiliations

  1. Institute for Sustainable and Renewable Resources, Institute for Advanced Learning and Research, 150 Slayton Avenue, Danville, VA, 24540, USA

    Zhiwu Li & Faith Campbell

  2. Departments of Horticulture and Forestry, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24060, USA

    Zhiwu Li

  3. Department of Biology, College of William and Mary, Williamsburg, VA, 23186, USA

    Katherine Mize

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  1. Zhiwu Li
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  2. Katherine Mize
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  3. Faith Campbell
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Correspondence to Zhiwu Li.

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Li, Z., Mize, K. & Campbell, F. Regeneration of daylily (Hemerocallis) from young leaf segments. Plant Cell Tiss Organ Cult 102, 199–204 (2010). https://doi.org/10.1007/s11240-010-9722-8

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  • DOI: https://doi.org/10.1007/s11240-010-9722-8

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