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Crystal structure of the Psb27 assembly factor at 1.6 Å: implications for binding to Photosystem II

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Abstract

The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of the Mn4Ca cluster catalysing water oxidation. To gain a better understanding of Psb27, we have determined in this study the crystal structure of the soluble domain of Psb27 from Thermosynechococcus elongatus to a resolution of 1.6 Å. The structure is a four-helix bundle, similar to the recently published solution structures of Psb27 from Synechocystis PCC 6803 obtained by nuclear magnetic resonance (NMR) spectroscopy. Importantly, the crystal structure presented here helps us resolve the differences between the NMR-derived structural models. Potential binding sites for Psb27 within PSII are discussed in light of recent biochemical data in the literature.

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Abbreviations

D1:

Photosystem II reaction centre subunit encoded by psbA

D2:

Photosystem II reaction centre subunit encoded by psbD

CP43 and CP47:

Photosystem II proximal light-harvesting subunits encoded by psbC and psbB, respectively

PsbO:

Photosystem II manganese-stabilising polypeptide

PsbV:

Cytochrome c-550

PsbU:

Photosystem II 12-kDa extrinsic protein

RC47:

Photosystem II complex lacking CP43

RCC1:

Monomeric photosystem II

RCC2:

Dimeric photosystem II

RMSD:

Root mean square deviation

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Acknowledgments

The authors thank the staff of SOLEIL Light Source for their help. FM, MB and PJN would like to thank the BBSRC (BB/E006388/1) and Engineering and Physical Sciences Research Council (EPSRC, EP/F00270X/1), and JK to the Czech Science Foundation (GACR, P501/11/0377) and Algatech (CZ.1.05/2.1.00/03.0110) for the respective financial supports. JWM holds a Biotechnology and Biological Sciences Research Council (BBSRC) David Phillips Fellowship (BB/F023308/1).

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Author notes

  1. Kenji Takasaka

    Present address: Division of Bioscience, Faculty of Science, Okayama University, Okayama, 700-8530, Japan

  2. Marko Boehm

    Present address: National Renewable Energy Laboratory, 16253 Denver West Parkway, FTLB-(190-04B), Golden, CO, 80401, USA

Authors and Affiliations

  1. Division of Molecular Biosciences, Imperial College London, Wolfson Biochemistry Building, South Kensington Campus, London, SW7 2AZ, UK

    Franck Michoux, Kenji Takasaka, Marko Boehm, Peter J. Nixon & James W. Murray

  2. Institute of Microbiology, Academy of Sciences, 379 81, Třeboň, Czech Republic

    Josef Komenda

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  1. Franck Michoux
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  2. Kenji Takasaka
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  4. Josef Komenda
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Correspondence to Franck Michoux.

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Michoux, F., Takasaka, K., Boehm, M. et al. Crystal structure of the Psb27 assembly factor at 1.6 Å: implications for binding to Photosystem II. Photosynth Res 110, 169–175 (2012). https://doi.org/10.1007/s11120-011-9712-7

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  • DOI: https://doi.org/10.1007/s11120-011-9712-7

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