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A Comparative Transcriptome Analysis Leads to New Insights into the Molecular Events Governing Root Formation in Mulberry Softwood Cuttings

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Abstract

Accelerated root formation is necessary for successful cuttage in a number of woody plants, such as mulberry (Morus alba L.). However, little information regarding the molecular mechanisms involved in this type of root formation is available. Here, we compared transcriptome changes during different root formation periods (0, 4, and 8 days) by conducting high-throughput sequencing to understand the molecular mechanisms of root formation in mulberry softwood cuttings. Approximately 88.2 million, 88.2 million, and 52.8 million transcriptome sequencing reads were obtained for the three time periods, 0, 4, and 8 days, respectively. These reads were then assembled into 61,687 (0 day), 61,651 (4 days), and 59,457 (8 days) contigs by utilizing the de novo method, and a large number of differentially expressed genes were identified at various developmental stages. These assembled contigs were annotated using Kyoto Encyclopedia of Genes and Genomes (KEGG), as well as the simple sequence repeat (SSR). Mulberry had a complex response to adventitious rooting. Genes that might be associated with mulberry stem cutting root formation were also identified here. Furthermore, the reliability of the sequencing results was validated by performing quantitative real-time polymerase chain reaction (qRT-PCR). These results provide a comprehensive background of the molecular biology of mulberry development, in particular regarding its stem cutting root formation development.

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Compliance with Ethical Standards

We have known the ethical responsibilities of authors and declare to comply with ethical standards. We have reviewed the final version of the manuscript and approved it for publication. To the best of our knowledge and belief, this manuscript has not been published in whole or in part nor is it being considered for publication elsewhere.

Conflict of Interest

The authors declare that they have no competing interests.

Human and animal studies

This article does not contain any studies with human or animal subjects.

Funding

This work was supported by China Agriculture Research System (Grant No. CARS-22).

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Authors and Affiliations

  1. Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, China

    Wei Du, Yueyuan Ban, Hao Nie, Zhuang Tang, Xiaolong Du & Jialing Cheng

  2. Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, 661101, Yunnan, China

    Wei Du

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  1. Wei Du
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  2. Yueyuan Ban
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  3. Hao Nie
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  4. Zhuang Tang
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  5. Xiaolong Du
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Correspondence to Jialing Cheng.

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Du, W., Ban, Y., Nie, H. et al. A Comparative Transcriptome Analysis Leads to New Insights into the Molecular Events Governing Root Formation in Mulberry Softwood Cuttings. Plant Mol Biol Rep 34, 365–373 (2016). https://doi.org/10.1007/s11105-015-0927-1

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