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Sequence Analysis of New S-RNase and SFB alleles in Japanese Apricot (Prunus mume)

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Abstract

As with other self-incompatible Prunus species, cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) display the S-RNase-based gametophytic self-incompatibility system. In this study, S-genotypes of ten Japanese apricot cultivars native to China were subjected to polyacrylamide gel electrophoresis (PAGE) analysis using an efficient Prunus S-RNase primer pair, Pru-C2 and PCE-R. In addition, three new S-RNase genes (S 34 , S 35 and S 36 -RNase) and six new SFB genes (PmSFB14, PmSFB18, PmSFB22, PmSFB24, PmSFB31 and PmSFB34) were identified and their sequences were characterized and deposited in the GenBank database.

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Acknowledgments

We gratefully acknowledge the Special Fund for Agro-Scientific Research in the Public Interest of the Ministry of Agriculture of China (201003058), the National Science Foundation of China (31101526) and the Jiangsu Province Agriculture Independent Innovation System Project [CX(12)2011] for providing financial support.

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  1. College of Horticulture, Nanjing Agricultural University, No. 1 Weigang, Nanjing City, 210095, Jiangsu Province, People’s Republic of China

    ZhiHong Gao, PeiPei Wang, WeiBing Zhuang & Zhen Zhang

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  1. ZhiHong Gao
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  2. PeiPei Wang
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  3. WeiBing Zhuang
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  4. Zhen Zhang
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Correspondence to ZhiHong Gao.

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Zhihong Gao and Peipei Wang are the co-first authors and contribute equally to this work.

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Gao, Z., Wang, P., Zhuang, W. et al. Sequence Analysis of New S-RNase and SFB alleles in Japanese Apricot (Prunus mume). Plant Mol Biol Rep 31, 751–762 (2013). https://doi.org/10.1007/s11105-012-0535-2

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