Abstract
Anther dehiscence, one of the essential steps in pollination and double fertilization, is regulated by a complex signaling pathway encompassing hormones and environmental factors. However, key components underlying the signaling pathway that regulate anther dehiscence remain largely elusive. Here, we isolated a rice mutant anther dehiscence defected 1 (Osadd1) that exhibited defects in anther dehiscence and glume open. Map-based cloning revealed that OsADD1 encoded a GARP (Golden2, ARR-B and Psr1) transcription factor. Sequence analysis showed that a single base deletion in Osadd1 mutant resulted in pre-termination of the GARP domain. OsADD1 was constitutively expressed in various tissues, with more abundance in the panicles. The major genes associated with anther dehiscence were affected in the Osadd1 mutant, and the expression level of the cellulose synthase-like D sub-family 4 (OsCSLD4) was significantly decreased. We demonstrate that OsADD1 regulated the expression of OsCSLD4 by binding to its promoter, and affects rice anther dehiscence.
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Acknowledgements
This research was supported by the National Transform Science and Technology Program (2016ZX08001004-002) and National Key Research and Development Program of China (2016YFD0101107, 2016YFD0101801), the Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in the Mid-lower Yangtze River, the Ministry of Agriculture, China, Jiangsu Plant Gene Engineering Research Center, the Jiangsu Collaborative Innovation Center for Modern Crop Production. This study is funded by China Postdoctoral Science Foundation. We are grateful to Professor Wenzhen Liu (China National Rice Research Institute, China) for providing the seeds of Oscsld4/nrl1 mutant.
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Y.J.X., S.M.Y. and Z.G.Z. were involved in conceiving the project, designing the experiments and analyzing the data; W.Y.K., Y.J.X., S.M.Y. and Y.C. were involving in the map-based cloning of the OsADD1 gene; Q.Y.T., W.T.B. and H.Z. were involved in the generation of the transgenic plants; C.L.W. was involved in OsADD1 transient expression in rice protoplasts; Y.J.X. was involved in LUC assay and EMSA; Y.J.X. was involved in writing this article; J.L. and C.M.W. were involved in revising this article; J.L., Z.G.Z. and J.M.W. were involved in data discussions.
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Xiao, Y., You, S., Kong, W. et al. A GARP transcription factor anther dehiscence defected 1 (OsADD1) regulates rice anther dehiscence. Plant Mol Biol 101, 403–414 (2019). https://doi.org/10.1007/s11103-019-00911-0
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DOI: https://doi.org/10.1007/s11103-019-00911-0