Photosynthetica 2017, 55(3):421-433 | DOI: 10.1007/s11099-016-0657-0

Relationship between photosynthetic pigments and chlorophyll fluorescence in soybean under varying phosphorus nutrition at ambient and elevated CO2

S. K. Singh1,2,*, V. R. Reddy1, D. H. Fleisher1, D. J. Timlin1
1 Crop Systems and Global Change Laboratory, USDA-ARS, Beltsville, USA
2 Wye Research and Education Center, University of Maryland, Beltsville, USA

To assess the relationship between chlorophyll (Chl) fluorescence (CF) and photosynthetic pigments, soybean was grown under varying phosphorus (P) nutrition at ambient and elevated CO2 (EC). The EC stimulated, but P deficiency decreased plant height, node numbers, and leaf area concomitantly with the rates of stem elongation, node addition, and leaf area expansion. Under P deficiency, CF parameters and pigments declined except that carotenoids (Car) were relatively stable indicating its role in photoprotection. The CF parameters were strongly related with Chl concentration but not with Chl a/b or Car. However, total Chl/Car showed the strongest association with CF parameters such as quantum efficiency and yield of photosystem II. This relationship was not affected by CO2 treatment. The high correlation between CF and total Chl/Car underscores the significance of the quantification of both, Chl and Car concentrations, to understand the photochemistry and underlying processes of photoprotection and mechanisms of excess energy dissipation in a given environment.

Additional key words: chlorophyll, carotenoids ratio; energy dissipation; photochemical quenching; relationship; response curve

Received: March 18, 2016; Accepted: July 15, 2016; Published: September 1, 2017  Show citation

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Singh, S.K., Reddy, V.R., Fleisher, D.H., & Timlin, D.J. (2017). Relationship between photosynthetic pigments and chlorophyll fluorescence in soybean under varying phosphorus nutrition at ambient and elevated CO2. Photosynthetica, 55(3), 421-433. doi: 10.1007/s11099-016-0657-0
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