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New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules

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ABSTRACT

Purpose

In general, the surface functionalization of polymeric nanoparticles is carried out by covalently bounding ligands to the nanoparticle surface. This process can cause a lack or decrease of the ligand specificity to its target receptor, besides the need of purification steps. We proposed a ligand-metal-chitosan-lecithin complex as a new strategy to functionalize the surface of biodegradable nanoparticles.

Methods

One pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules was carried out by self-assembly and interfacial reactions. Particle sizing techniques, lipid peroxidation and molecular recognition by enzyme linked immuno sorbent assays were carried out.

Results

The selected formulation had unimodal size distribution with mean diameter of about 130 nm. The metals in the complex did not enhance the oxidative stress, and the scFv anti-LDL(−)-functionalized nanocapsules recognized LDL(−) and did not react with native LDL indicating the maintenance of the active site of the fragment.

Conclusions

The one pot synthesis, using the ligand-metal-chitosan-lecithin complex to functionalize the surface of the biodegradable nanocapsules, maintained the active site of the antibody fragment making the device interesting for applications in nanomedicine.

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Abbreviations

DLS:

Dynamic light scattering

ELISA:

Enzyme linked immuno sorbent assay

LD:

Laser diffractometry

LDE:

Laser Doppler electrophoresis

LDL:

Low density lipoprotein

LDL(−):

Electronegative low density lipoprotein

LNC:

Lipid-core nanocapsules

MCMN:

Metal complex multi-wall nanocapsules

MDA:

Malondialdehyde

MN:

Multi-wall nanocapsules

nLDL:

Native low density lipoprotein

NTA:

Nanoparticle tracking analysis

PCL:

Poly(ε-caprolactone)

PDI:

Polydispersity index

Phe:

Phenylalanine

scFv anti-LDL(−):

Anti-electronegative LDL single-chain fragment variable

SPAN:

Polydispersity

TBA:

Thiobarbituric acid

TBARS:

Thiobarbituric acid reactive substances

TEM:

Transmission electron microscopy

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ACKNOWLEDGMENTS AND DISCLOSURES

EAB thanks CAPES/Brazil for his fellowship. The authors thank PRONEX e PRONEM FAPERGS/CNPq, FAPESP, INCT-IF/CNPq, CNPq/Brasilia/Brazil, Universal CNPq/MCTI and Rede Nanotecnologia Farmaceutica CAPES (Brazil) for grant financial supports.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Eduardo A. Bender, Márcia D. Adorne, Letícia M. Colomé & Sílvia S. Guterres

  2. Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil

    Marcela F. Cavalcante & Dulcinéia S. P. Abdalla

  3. Departamento de Química Orgânica and Programa de Pós-Graduação em Nanotecnologia Farmacêutica, Instituto de Química, Universidade Federal do Rio Grande do Sul, PBOX 15003, Porto Alegre, 91501-970, RS, Brazil

    Adriana R. Pohlmann

Authors
  1. Eduardo A. Bender
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  2. Marcela F. Cavalcante
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  3. Márcia D. Adorne
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  4. Letícia M. Colomé
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  5. Sílvia S. Guterres
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  6. Dulcinéia S. P. Abdalla
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Corresponding author

Correspondence to Adriana R. Pohlmann.

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Bender, E.A., Cavalcante, M.F., Adorne, M.D. et al. New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules. Pharm Res 31, 2975–2987 (2014). https://doi.org/10.1007/s11095-014-1392-5

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  • DOI: https://doi.org/10.1007/s11095-014-1392-5

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