Abstract
Considering the important role of oxidative stress in the pathogenesis of several neurological diseases, and the growing evidence of the presence of compounds with antioxidant properties in the plant extracts, the aim of the present study was to investigate the antioxidant capacity of three plants used in Brazil to treat neurological disorders: Melissa officinalis, Matricaria recutita and Cymbopogon citratus. The antioxidant effect of phenolic compounds commonly found in plant extracts, namely, quercetin, gallic acid, quercitrin and rutin was also examined for comparative purposes. Cerebral lipid peroxidation (assessed by TBARS) was induced by iron sulfate (10 μM), sodium nitroprusside (5 μM) or 3-nitropropionic acid (2 mM). Free radical scavenger properties and the chemical composition of plant extracts were assessed by 1′-1′ Diphenyl-2′ picrylhydrazyl (DPPH) method and by Thin Layer Chromatography (TLC), respectively. M. officinalis aqueous extract caused the highest decrease in TBARS production induced by all tested pro-oxidants. In the DPPH assay, M. officinalis presented also the best antioxidant effect, but, in this case, the antioxidant potencies were similar for the aqueous, methanolic and ethanolic extracts. Among the purified compounds, quercetin had the highest antioxidant activity followed by gallic acid, quercitrin and rutin. In this work, we have demonstrated that the plant extracts could protect against oxidative damage induced by various pro-oxidant agents that induce lipid peroxidation by different process. Thus, plant extracts could inhibit the generation of early chemical reactive species that subsequently initiate lipid peroxidation or, alternatively, they could block a common final pathway in the process of polyunsaturated fatty acids peroxidation. Our study indicates that M. officinalis could be considered an effective agent in the prevention of various neurological diseases associated with oxidative stress.
References
Frei B (1994) Reactive oxygen species and antioxidant vitamins: mechanisms of action. Am J Med 97:5S–13S
Silva CG, Herdeiro RS, Mathias CJ, Panek AD, Silveira CS, Rodrigues VP, Rennó MN, Falcão DQ, Cerqueira DM, Minto ABM (2005) Evaluation of antioxidant activity of Brazilian plants. Pharm Res 52:229–233
Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239–247
Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J (2004) Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 266:37–56
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40
Simonian NA, Coyle JT (1996) Oxidative stress in neurodegenerative diseases. Annu Rev Pharmacol Toxicol 36:83–106
Gilgun-Sherki Y, Melamed E, Offen D (2001) Oxidative stress induced-neurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology 40:959–975
Bastianetto S, Quirion R (2002) Natural extracts as possible protective agents of brain aging. Neurobiol Aging 23:891–897
Ávila DS, Gubert P, Palma A, Colle D, Alves D, Nogueira CW, Rocha JBT, Soares FAA (2008) An organotellurium compound with antioxidant activity against excitotoxic agents without neurotoxic effects in brain of rats. Brain Res Bull 76:114–123
Wagner C, Fachinetto R, Dalla Corte CL, Brito VB, Severo D, Dias GOC, Morel AF, Nogueira CW, Rocha JBT (2006) Quercitrin, a glycoside form of quercetin, prevents lipid peroxidation in vitro. Brain Res 1107:192–198
Williams RJ, Spencer JPE, Rice-Evans C (2004) Flavonoids: antioxidants or signalling molecules? Free Radical Biol Med 36:838–849
Patel R, Garg R, Erande S, Maru GB (2007) Chemopreventive herbal anti-oxidants: current status and future perspectives. J Clin Biochem Nutr 40:82–91
Cui K, Luo XL, Xu KY, Murthy MRV (2004) Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants. Prog Neuropsychopharmacol Biol Psychiatry 28:771–799
Evans DA, Hirsch JB, Dushenkov S (2006) Phenolics, inflammation and nutrigenomics. J Sci Food Agric 86:2503–2509
Mentreddy SR (2007) Review––medicinal plant species with potential antidiabetic properties. J Sci Food Agric 87:743–750
Leite JR, Seabra ML, Maluf E, Assolant K, Suchecki D, Tufik S, Klepacz S, Calil HM, Carlini EA (1986) Pharmacology of lemongrass (Cymbopogon citratus Stapf). III. Assessment of eventual toxic, hypnotic and anxiolytic effects on humans. J Ethnopharmacol 17:75–83
Velioglu YS, Mazza G, Gao L, Oomach BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem 46:4113–4117
Oboh G, Rocha JBT (2007) Polyphenols in red pepper [Capsicum annuum var. aviculare (Tepin)] and their protective effect on some pro-oxidants induced lipid peroxidation in brain and liver. Eur Food Res Tech 225:239–247
Oboh G, Puntel RL, Rocha JBT (2007) Hot pepper (Capsicum annuum, Tepin and Capsicum chinese, Habanero) prevents Fe2+ -induced lipid peroxidation in brain - in vitro. Food Chem 102:178–185
Sabir SM, Maqsood H, Ahmed SD, Shah AH, Khan MQ (2005) Chemical and nutritional constituents of sea buckthorn (Hippophae rhamnoides ssp. turkestanica) berries from Pakistan. Ital J Food Sci 17:455–462
Dreostic IE, Wargovich MJ, Yang CS (1997) Inhibition of carcinogenesis by tea: the evidence from experimental studies. Crit Rev Food Sci Nutr 37:761–770
Jankun J, Selman SH, Swiercz R, Skrzypczak-Jankun E (1997) Why drinking green tea could prevent cancer. Nature 387:561
Wiseman SA, Balentine DA, Frei B (1997) Antioxidants in tea. Crit Rev Food Sci Nutr 37:705–718
Hertog MGL, Hollman PCH, van de Putte B (1993) Content of potentially anticarcinogenic flavonids of tea infusions, wines, and fruit juices. J Agric Food Chem 41:1242–1246
Au Kono S, Shinchi K, Wakabayashi K, Honjo S, Todoroki I, Sakura Y, Imanishi K, Nishikawa H, Ogawa S, Katsurada M (1996) Relation of green tea consumption to serum lipids and lipoproteins in Japanese men. J Epidemio 6:128–133
Tijburg LBM, Mattern T, Folts JD, Weisgerber UM, Katan MB (1997) Tea flavonoids and cardiovascular diseases: a review. Crit Rev Food Sci Nutr 37:771–785
Pietrovski EF, Rosa KA, Facundo VA, Rios K, Marques MCA, Santos ARS (2006) Antinociceptive properties of the ethanolic extract and of the triterpene 3 h, 6 h, 16 h-trihidroxilup-20(29)-ene obtained from the flowers of Combretum leprosum in mice. Pharmacol Biochem Behav 83:90–99
Carlini EA (2003) Plants and the central nervous system. Pharmacol Biochem Behav 75:501–512
dos Santos-Neto LL, de Vilhena Toledo MA, Medeiros-Souza P, de Souza GA (2006) The use of herbal medicine in Alzheimer’s disease––a systematic review. Ev-Bas Comp Alt Med 3:441–445
de Sousa AC, Alviano DS, Blank AF, Alves PB, Alviano CS, Gattass CR (2004) Melissa officinalis L essential oil: antitumoral and antioxidant activities. J Pharm Pharmacol 56:677–681
Marongiu B, Porcedda S, Piras A, Rosa A, Deiana M, Dessi MA (2004) Antioxidant activity of supercritical extract of Melissa officinalis subsp. officinalis and Melissa officinalis subsp. inodora. Phytother Res 18:789–792
Perry EK, Pickering AT, Wang WW, Houghton PJ, Perry NS (1999) Medicinal plants and Alzheimer’s disease: from ethnobotany to phytotherapy. J Pharm Pharmacol 51:527–534
Carlini EA, Contar JDP, Silva-Filho AR, Silveira-Filho NG, Frochtengarten ML, Bueno OF (1986) Pharmacology of lemongrass (Cymbopogon citratus Stapf) I. Effects of teas prepared from the leaves on laboratory animals. J Ethnopharmacol 17:37–64
Avallone R, Zanoli P, Puia G, Kleinschnitz M, Schreier P, Baraldi M (2000) Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla. Biochem Pharmacol 59:1387–1394
Zanoli P, Avallone R, Baraldi M (2000) Behavioral characterization of the flavonoids apigenin and chrysin. Fitoterapia 71:S117–S123
Fidler P, Loprinzi CL, O’Fallon JR, Leitch JM, Lee JK, Hayes DL, Novotny P, Clemens-Schutjer D, Bartel J, Michalak JC (1996) Prospective evaluation of a chamomile mouthwash for prevention of 5-FU-induced oral mucositis. Cancer 77:522–525
Morel AF, Dias GO, Porto C, Simionatto C, Stuker CZ, Dalcol II (2006) Antimicrobial activity of extractives of Solidago microglossa. Fitoterapia 77:453–455
Puntel RL, Roos DH, Grotto D, Garcia SC, Nogueira CW, Rocha JB (2007) Antioxidant properties of Krebs cycle intermediates against malonate pro-oxidant activity in vitro: a comparative study using the colorimetric method and HPLC analysis to determine malondialdehyde in rat brain homogenates. Life Sci 81:51–62
Ohkawa H, Ohishi H, Yagi K (1979) Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358
Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Paerk SH, Kim SK (2002) Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci 153:1161–1168
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Meth Enzymol 299:152–178
Pachaly P (1999) DC-Atlas–Dünnschicht-Chromatographie in der Apotheke. Wissenschaftliche Verlagsgesellschaft, Stuttgart
Stahl E, Schild W (1981) Pharmazeutische Biologie–Drogenanalyse II: Inhaltsstoffe und Isolierung. Gustav Fischer, Stuttgart
Pereira MA, Grubbs CJ, Barnes LH, Li H, Olson GR (1996) Effect of the phytochemicals, curcumin and quercetin upon azomethane-induced: cancer and 7, 12-dimethylbenz(a)anthracene-induced mammary cancer in rats. Carcinogenesis 17:1305–1311
Yang CS, Kim S, Yang GY, Lee MJ, Liao J (1999) Inhibition of the carcinogenesis by tea; bioavailability of the tea polyphenols and mechanisms of the action. Pro Soc Exp Biol Med 220:213–217
Thompson LU (2000) Lignans and isoflavones. In: Eisenbrand G, Dayan AD, Elias PS, Grunow W, Schlatter J (eds) Carcinogenic/anticarcinogenic factors in foods. Dtsch. Forsch. Gem., Ger. Wiley-VCH, Germany
Atoui AK, Mansouri A, Boskou G, Kefalas P (2005) Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chem 89:27–36
Geetha T, Malhotra V, Chopra K, Kaur IP (2005) Antimutagenic and antioxidant/prooxidant activity of Quercetin. Indian J Exp Biol 43:61–67
Bostanci MO, Bagirici F (2008) Neuroprotective effect of aminoguanidine on iron-induced neurotoxicity. Brain Res Bull 76:57–62
Fraga CG, Oteiza PI (2002) Iron toxicity and antioxidant nutrients. Toxicology 80:23–32
Aisen P, Wessling-Resnick M, Leibold EA (1999) Iron metabolism. Curr Opin Chem Biol 3:200–206
Qian ZM, Wang Q, Pu Y (1997) Brain iron and neurological disorders. Chin Med J 110:455–458
Swaiman KF (1991) Hallervorden-Spatz and brain iron metabolism. Arch Neurol 48:1285–1293
Arnold WP, Longneeker DE, Epstein RM (1984) Photodegradation of sodium nitroprusside: biologic activity and cyanide release. Anesthesiology 61:254–260
Bates JN, Baker MT, Guerra R, Harrison DG (1990) Nitric oxide generation from nitroprusside by vascular tissue. Biochem Pharmacol 42:S157–S165
Huie RE, Padmaja S (1993) The reaction of NO with superoxide. Free Radic Res Commun 18:195–199
Pryor WA, Squadrito GL (1995) The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide. Am J Physiol 268:L699–L722
Alston TA, Mela L, Bright HJ (1977) 3-Nitropropionate, the toxic substance of Indiofera, is a suicide inactivator of succinate dehydrogenase. Proc Natl Acad Sci USA 74:3767–3771
Brand Williams W, Cuvelier MC, Berset C (1995) Use of a free-radical method to evaluate antioxidant activity. LWT 28:25–30
Hatano T, Edmatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T (1989) Effects of the interaction of tannins with coexisting substances. VI Effect of tannins and related polyphenols on superoxide anion radicals and on DPPH. Chem Pharm Bull 37:2016–2021
Yasuda T, Inaba A, Ohmori M, Endo T, Kubo S, Ohsawa K (2000) Urinary metabolites of gallic acid in rats and their radical scavenging effect on DPPH. J Nat Prod 63:1444–1446
Rice-Evans C, Miller N (1997) Measurement of the antioxidant status of dietary constituents, low density lipoproteins and plasma. Prostaglandins Leukotv Essent Fatty Acids 57:499–505
Vinson JA, Dabbagh YA, Serry MM, Jang J (1995) Plant flavonoids, especially tea flavonoids, are powerful antioxidants using an in vitro oxidation model for heart disease. J Agric Food Chem 43:2800–2802
Saija A, Scalese M, Lanza M, Marzullo D, Bonina F, Castelli F (1995) Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic Biol Med 19:481–486
Acknowledgements
The financial support by CAPES/SAUX/PROAP, VITAE Fundation, CNPq, FAPERGS, ICTP and FINEP research grant “Rede Instituto Brasileiro de Neurociência (IBN-Net)” # 01.06.0842-00 is gratefully acknowledged.
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Pereira, R.P., Fachinetto, R., de Souza Prestes, A. et al. Antioxidant Effects of Different Extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus . Neurochem Res 34, 973–983 (2009). https://doi.org/10.1007/s11064-008-9861-z
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DOI: https://doi.org/10.1007/s11064-008-9861-z