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MicroRNA-132 targets PEA-15 and suppresses the progression of astrocytoma in vitro

  • Laboratory Investigation
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Abstract

Gliomas are highly malignant tumors, the most common of which are astrocytomas. A growing number of studies suggest that dysregulation of miRNAs is a frequent event contributing to the pathogenesis of gliomas. In this study, we found that over-expression of miR-132 inhibited cell proliferation and migration and triggered apoptosis, while knockdown of miR-132 showed opposite effects. PEA-15 was identified as a direct target of miR-132. Reintroduction of PEA-15 without 3′UTR region reversed the inhibitory effects of miR-132 on cell proliferation, migration, and apoptosis. MiR-132 was inversely correlated with the PEA-15 expression. CREB (cAMP response element binding protein) and KLF (Krüppel-like factor 8) were conformed as transcription factors of miR-132, which bidirectionally regulate the expression of miR-132. Our study suggests that miR-132 is an important tumor suppressor of astrocytoma progression by targeting PEA-15, while CREB and KLF can modulate the expression of miR-132, thus providing new insight into the molecular mechanisms underlying astrocytoma progression in vitro.

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Acknowledgments

This work was supported by China Postdoctoral Science Foundation Funded Project (2015 M572336).

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Authors and Affiliations

  1. Department of Neurobiology, Southern Medical University, Guangzhou, 510515, Guangdong, People’s Republic of China

    Fei Geng, Jian-Lin Wu, Zhi-Ping Liang, Zhuo-Li Duan & Xi Gu

  2. Guangdong Province Key Laboratory of Neuroplasticity of Guangdong Higher Education Institutes, Guangzhou, 510515, Guangdong, People’s Republic of China

    Fei Geng, Jian-Lin Wu, Zhi-Ping Liang, Zhuo-Li Duan & Xi Gu

  3. Department of Pathology, Southern Medical University, Guangzhou, 510515, Guangdong, People’s Republic of China

    Gui-Feng Lu

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  1. Fei Geng
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Correspondence to Xi Gu.

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Geng, F., Wu, JL., Lu, GF. et al. MicroRNA-132 targets PEA-15 and suppresses the progression of astrocytoma in vitro. J Neurooncol 129, 211–220 (2016). https://doi.org/10.1007/s11060-016-2173-2

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