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Long non-coding genes implicated in response to stripe rust pathogen stress in wheat (Triticum aestivum L.)

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Abstract

The non-protein-coding genes have been reported as a critical control role in the regulation of gene expression in abiotic stress. We previously identified four expressed sequence tags numbered S18 (EL773024), S73 (EL773035), S106 (EL773041) and S108 (EL773042) from a SSH-cDNA library of bread wheat Shaanmai 139 infected with Puccinia striiformis f. sp. tritici (Pst). Here, we isolated four cDNA clones and referred them as TalncRNA18, TalncRNA73, TalncRNA106 and TalncRNA108 (GenBank: KC549675–KC549678). These cDNA separately consisted of 1,393, 667, 449 and 647 nucleotides but without any open reading frame. The alignment result showed that TalncRNA18 is a partial cDNA of E3 ubiquitin-protein ligase UPL1-like gene, TalncRNA73 is an antisense transcript of hypothetical protein, TalncRNA108 is a homolog to RRNA intron-encoded homing endonuclease, and lncRNA106 had no similarly sequence. Quantitative RT-PCR studies confirmed that these four lncRNAs were differentially expressed in three near isogenic lines. TalncRNA108 was significantly stepwise decreased at early stage of inoculation with Pst, while the others were upregulated, especially at 1 and 3 dpi (days post-inoculation). Using Chinese Spring nulli-tetrasomic lines and its ditelosomic lines, TalncRNA73 and TalncRNA108 were located to wheat chromosome 7A and the short arm of chromosome 4B, respectively, while TalncRNA18 and TalncRNA106 were located to chromosome 5B. Comparing the sequence of DNA and cDNA of four lncRNAs with polymerase chain reaction primers, the results showed that all of them have no introns. The kinetics analyses of lncRNAs expression as a result of pathogen challenge in immune resistant genotype indicated that they may play the roles of modulating or silencing the protein-coding gene into pathogen-defence response.

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Acknowledgments

This work was financially supported by National Key Basic Research Program of China (2013CB127700), the National Natural Science Foundation of China (31371612) and the Fundamental Research Funds for the Central Universities (Northwest A&F University, QN2011002).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Area, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Hong Zhang, Xueyan Chen, Changyou Wang, Zhongyang Xu, Yajuan Wang, Xinlun Liu & Wanquan Ji

  2. State Key Laboratory of Crop Stress Biology for Arid Area, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Hong Zhang & Zhensheng Kang

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  1. Hong Zhang
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  2. Xueyan Chen
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Correspondence to Hong Zhang, Zhensheng Kang or Wanquan Ji.

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Zhang, H., Chen, X., Wang, C. et al. Long non-coding genes implicated in response to stripe rust pathogen stress in wheat (Triticum aestivum L.). Mol Biol Rep 40, 6245–6253 (2013). https://doi.org/10.1007/s11033-013-2736-7

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  • DOI: https://doi.org/10.1007/s11033-013-2736-7

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