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Targeted spatio-temporal expression based characterization of state of infection and time-point of maximum defense in wheat NILs during leaf rust infection

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Abstract

Leaf rust, caused by the fungus Puccinia triticina, is the most devastating disease of wheat worldwide, which sometimes becomes epidemic. The pathogen evolves into new strains, making its control difficult. Though more than 60 leaf rust resistant genes are now known, only limited insight is available into the molecular mechanism involved in this host pathogen interaction. In the present study, quantitative real-time PCR based differential gene expression profiling was examined for five target genes encoding for chitinase3, β-1,3/1,4 glucanase, thaumatin-like protein, peroxidase2 and mitogen activated protein kinase1 to unravel their coordinated action during compatible and incompatible interaction, to inhibit the pathogen progression and to identify the time-period of maximum defense activity. Spatio-temporal expression profiling suggested that the maximum defense activity occurred at 12–24 hours post inoculation, whereas the state of infection and degree of resistance was predicted using coordinated unique expression signatures of target genes. The significant differences of targeted gene expression between resistant mock inoculated, resistant infected and susceptible infected plants were evaluated using t test at significance level of p < 0.01. The differences occurred can be attributed to the presence of seedling leaf rust resistance Lr28 gene, which facilitated prevention of leaf rust infection in resistant wheat plants.

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References

  1. Long DL, Kolmer JA (1989) A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology 79:525–529

    Article  Google Scholar 

  2. Roelf AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concept and methods of disease management. CIMMYT, Mexico

    Google Scholar 

  3. McIntosh RA, Wellings CR, Park RF (1995) Wheat rust: an atlas of resistance genes. CSIRO, Australia

    Google Scholar 

  4. Saari EE, Prescott JM (1985) World distribution in relation to economic losses. In: Roelfs AP, Bushnell WR (eds) The cereal rusts: diseases, distribution, epidemiology, and control. Academic Press, Orlando, pp 259–298

    Google Scholar 

  5. Bolton MD, Kolmer JA, Garvin DF (2008) Wheat leaf rust caused by Puccinia triticina. Mol Plant Pathol 9:563–575

    Article  PubMed  Google Scholar 

  6. Xu J, Linning R, Fellers J, Dickinson M, Zhu W, Antonov I, Joly DL, Donaldson ME, Eilam T, Anikster Y, Banks T, Munro S, Mayo M, Wynhoven B, Ali J, Moore R, McCallum B, Borodovsky M, Saville B, Bakkeren G (2011) Gene discovery in EST sequences from the wheat leaf rust fungus Puccinia triticina sexual spores, asexual spores and haustoria, compared to other rust and corn smut fungi. BMC Genomics 12:161

    Article  PubMed  CAS  Google Scholar 

  7. Dixon RA, Harrison MJ, Lamb CJ (1994) Early events in the activation of plant defense responses. Annu Rev Phytopathol 32:479–501

    Article  CAS  Google Scholar 

  8. Orczyk W, Dmochowska-Boguta M, Czembor HJ, Nadolska-Orczyk A (2010) Spatiotemporal patterns of oxidative burst and micronecrosis in resistance of wheat to brown rust infection. Plant Pathol 59:567–575

    Article  Google Scholar 

  9. Bozkurt TO, Mcgrann GRD, Maccormack R, Boyd LA, Akkaya MS (2010) Cellular and transcriptional responses of wheat during compatible and incompatible race-specific interactions with Puccinia striiformis f. sp. tritici. Mol Plant Pathol 11:625–640

    PubMed  CAS  Google Scholar 

  10. Wang X, Liu W, Chen X, Tang C, Dong Y, Ma J, Huang X, Wei G, Han Q, Huang L, Kang Z (2010) Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction. BMC Plant Biol 10:9

    Article  PubMed  Google Scholar 

  11. Bent AF, Mackey D (2007) Elicitors, Effectors, and R Genes: the new paradigm and a lifetime supply of questions. Annu Rev Phytopathol 45:399–436

    Article  PubMed  CAS  Google Scholar 

  12. Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363

    Article  PubMed  CAS  Google Scholar 

  13. Lagudah ES (2011) Molecular genetics of race non-specific rust resistance in wheat. Euphytica 179:81–91

    Article  Google Scholar 

  14. Zhang L, Meakin H, Dickinson M (2003) Isolation of genes expressed during compatible interactions between leaf rust (Puccinia triticina) and wheat using cDNA-AFLP. Mol Plant Pathol 4:469–477

    Article  PubMed  CAS  Google Scholar 

  15. Dhariwal R, Vyas S, Bhaganagare GR, Jha SK, Khurana JP, Tyagi AK, Prabhu KV, Balyan HS, Gupta PK (2011) Analysis of differentially expressed genes in leaf rust infected bread wheat involving seedling resistance gene Lr28. Funct Plant Biol 38:479–492

    CAS  Google Scholar 

  16. Thara VK, Fellers JP, Zhou JM (2003) In planta induced genes of Puccinia triticina. Mol Plant Pathol 4:51–56

    Article  PubMed  CAS  Google Scholar 

  17. Hu G, Linning R, McCallum B, Banks T, Cloutier S, Butterfield Y, Liu J, Kirkpatrick R, Stott J, Yang G, Smailus D, Jones S, Marra M, Schein J, Bakkeren G, Yamazaki Y (2007) Generation of a wheat leaf rust, Puccinia triticina, EST database from stage-specific cDNA libraries. Mol Plant Pathol 8:451–467

    Article  PubMed  CAS  Google Scholar 

  18. Fofana B, Banks TW, McCallum B, Strelkov SE, Cloutier S (2007) Temporal gene expression profiling of the wheat leaf rust pathosystem using cDNA microarray reveals differences in compatible and incompatible defence pathways. Int J Plant Genomics 2007:17542

    PubMed  Google Scholar 

  19. Poole RL, Barker GL, Werner K, Biggi GF, Coghill J, Gibbings JG, Berry S, Dunwell JM, Edwards KJ (2008) Analysis of wheat SAGE tags reveals evidence for widespread antisense transcription. BMC Genomics 9:475

    Article  PubMed  Google Scholar 

  20. Paolacci AR, Tanzarella OA, Porceddu E, Ciaffi M (2009) Identification and validation of reference genes for quantitative RT-PCR normalization in wheat. BMC Mol Biol 10:11

    Article  PubMed  Google Scholar 

  21. Hu G, Rijkenberg FHJ (1998) Scanning electron microscopy of early infection structure formation by Puccinia recondita f. sp. tritici on and in susceptible and resistant wheat lines. Mycol Res 102:391–399

    Article  Google Scholar 

  22. Yu X, Wang X, Wang C, Chen X, Qu Z, Yu X, Han Q, Zhao J, Guo J, Huang L, Kang Z (2010) Wheat defense genes in fungal (Puccinia striiformis) infection. Funct Integr Genomics 10:227–239

    Article  PubMed  CAS  Google Scholar 

  23. Rieu I, Powers SJ (2009) Real-time quantitative RT-PCR: design, calculations and statistics. Plant Cell 21:1031–1033

    Article  PubMed  CAS  Google Scholar 

  24. Marais F, Marais A, McCallum B, Pretorius Z (2009) Transfer of leaf rust and stripe rust resistance genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to common wheat. Crop Sci 49:871–879

    Article  CAS  Google Scholar 

  25. Cherukuri DP, Gupta SK, Charpe A, Koul S, Prabhu KV, Singh RB, Haq QMR (2005) Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica 143:19–26

    Article  CAS  Google Scholar 

  26. Kumar AA, Raghavaiah P (2004) Effect of the leaf rust resistance gene Lr28 on grain yield and bread-making quality of wheat. Plant Breed 123:35–38

    Article  CAS  Google Scholar 

  27. Kaur S, Bansal UK, Khanna R, Saini RG (2008) Genetics of leaf and stripe rust resistance in a bread wheat cultivar Tonichi. J Genet 87:191–194

    Article  PubMed  Google Scholar 

  28. Eckardt NA (2008) Chitin signalling in plants: insights into the perception of fungal pathogens and rhizobacterial symbionts. Plant Cell 20:241–243

    Article  PubMed  CAS  Google Scholar 

  29. Munch-Garthoff S, Neuhaus J-M, Boller T, Kimmerling B, Kogel K–H (1997) Expression of beta-1,3-glucanase and chitinase in healthy, stem-rust-affected and elicitor-treated near-isogenic wheat lines showing Sr5 -or Sr24 -specified race-specific rust resistance. Planta 201:235–244

    Article  PubMed  CAS  Google Scholar 

  30. Rajam MV, Chandola N, Goud PS, Singh D, Kashyap V, Choudhary ML, Sihachakr D (2007) Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses in transgenic tobacco plants. Biol Plant 51:135–141

    Article  CAS  Google Scholar 

  31. Manickavelu A, Kawaura K, Oishi K, Kohara Y, Shin-IT, Yahiaoui N, Keller B, Suzuki A, Yano K, Ogihara Y (2010) Comparative gene expression analysis of susceptible and resistant Near-Isogenic Lines in common wheat infected by Puccinia triticina. DNA Res 17:211–222

    Article  PubMed  CAS  Google Scholar 

  32. Rodriguez MCS, Petersen M, Mundy J (2010) Mitogen activated protein kinase signaling in plants. Annu Rev Plant Biol 61:621–649

    Article  PubMed  CAS  Google Scholar 

  33. Asthir B, Koundal A, Bains NS, Mann SK (2010) Stimulation of antioxidative enzymes and polyamines during stripe rust disease of wheat. Biol Plant 54:329–333

    Article  CAS  Google Scholar 

  34. Bandopadhyay R, Haque I, Singh D, Mukhopadhyay K (2009) Levels and stability of expression of transgene. In: Kole C, Michler CH, Abott AG, Hall TC (eds) Transgenic crop plants: principles and development. Springer, Heidelberg, pp 145–186

    Google Scholar 

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Acknowledgments

We are grateful to the Government of Jharkhand, Department of Agriculture for providing infrastructure development fund (5/B.K.V/Misc/12/2001) and to the Department of Biotechnology, Government of India for providing financial support (grant no. BT/PR6037/AGR/02/308/05).

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Authors and Affiliations

  1. Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi, 835215, Jharkhand, India

    Dharmendra Singh, Rajib Bandopadhyay & Kunal Mukhopadhyay

  2. National Phytotron Facility, Indian Agriculture Research Institute, New Delhi, 110012, India

    Govindraj Bhaganagare & Kumble Vinod Prabhu

  3. Department of Genetics and Plant Breeding, Chaudhary Charan Singh University, Meerut, 250004, India

    Pushpendra Kumar Gupta

Authors
  1. Dharmendra Singh
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  2. Govindraj Bhaganagare
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  3. Rajib Bandopadhyay
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  4. Kumble Vinod Prabhu
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  5. Pushpendra Kumar Gupta
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  6. Kunal Mukhopadhyay
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Correspondence to Kunal Mukhopadhyay.

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Singh, D., Bhaganagare, G., Bandopadhyay, R. et al. Targeted spatio-temporal expression based characterization of state of infection and time-point of maximum defense in wheat NILs during leaf rust infection. Mol Biol Rep 39, 9373–9382 (2012). https://doi.org/10.1007/s11033-012-1801-y

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  • DOI: https://doi.org/10.1007/s11033-012-1801-y

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