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A novel 33-bp insertion in the promoter of TaMFT-3A is associated with pre-harvest sprouting resistance in common wheat

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Abstract

TaMFT-3A on wheat chromosome 3AS, encoding a phosphatidyl ethanolamine-binding protein, was previously determined to be a candidate gene underlying the major quantitative trait locus (QTL) for pre-harvest sprouting (PHS) resistance. Three single nucleotide polymorphisms (SNPs) (−222, +646, and +666) and one insertion/deletion (InDel) variation at TaMFT-3A locus are associated with PHS resistance. In the present study, we detected a novel 33-bp InDel in the promoter (−194) of TaMFT-3A and developed a gene-specific marker (MFT-A2). Linkage mapping indicated that MFT-A2 co-segregated with a major QTL on chromosome 3AS for PHS resistance in the recombinant inbred line population from the cross of Annong 0711 × Henong 825, explaining 17.79 and 14.22% of phenotypic variations in 2016 and 2017, respectively. Association analysis also showed that the InDel was significantly associated with PHS resistance using 260 current wheat varieties (lines) and 183 Chinese mini-core wheat collections across environments. TaMFT-3A expression levels in genotypes with the resistance allele TaMFT-3Aa were significantly higher than those in varieties with the susceptible allele TaMFT-3Ab. Moreover, TaMFT-3A transcription was up-regulated in high-temperature and abscisic acid treatments, but down-regulated in low-temperature and gibberellic acid treatments. Notably, PHS resistance of varieties simultaneously carrying favorable haplotypes of SNP-222, SNP646/666, TaMFT-A1, and TaMFT-3A (designated Hap-11) was significantly higher than that of other haplotypes across environments. This study provides useful information for the use of the MFT-A2 marker for improvement of PHS resistance in wheat breeding, and for our better understanding of TaMFT-3A regulatory mechanism for PHS resistance.

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Acknowledgments

We thank Prof. Ji-Zeng Jia for kindly providing the 183 Chinese mini-core collections. We also thank Prof. Ray Rose for revising the manuscript.

Funding

This work was supported by grants from the National Natural Science Foundation of China (31401372), the China Agriculture Research System (CARS-03), the promotion project of high education of Anhui province “Team construction of high level teacher of crop discipline,” Wheat genetics and breeding research platform innovation team of Anhui’s University, Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), the Agriculture Research System of Anhui Province (AHCYTX-02), and the introduced leading talent research team for Universities in Anhui Province.

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  1. Hao Jiang and Liang-Xia Zhao contributed equally to this work.

Authors and Affiliations

  1. College of Agronomy, Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, Ministry of Agriculture, Anhui Agricultural University, Hefei, 230036, Anhui, People’s Republic of China

    Hao Jiang, Liang-Xia Zhao, Xue-Jian Chen, Jia-Jia Cao, Zeng-Yun Wu, Kai Liu, Can Zhang, Wen-Xin Wei, Hong-Yong Xie, Li Li, Yi-Gang Gan, Jie Lu, Cheng Chang, Hai-Ping Zhang, Xian-Chun Xia & Chuan-Xi Ma

  2. Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

    Xian-Chun Xia & Shi-He Xiao

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  1. Hao Jiang
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Correspondence to Hai-Ping Zhang or Chuan-Xi Ma.

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Jiang, H., Zhao, LX., Chen, XJ. et al. A novel 33-bp insertion in the promoter of TaMFT-3A is associated with pre-harvest sprouting resistance in common wheat. Mol Breeding 38, 69 (2018). https://doi.org/10.1007/s11032-018-0830-1

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