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Mendelization and fine mapping of a bread wheat spot blotch disease resistance QTL

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Abstract

The wheat (Triticum aestivum L.) quantitative trait locus (QTL) QSb.bhu-5B, which determines resistance to spot blotch (causative pathogen Bipolaris sorokiniana), was mapped to an interval of 0.62 cM on chromosome arm 5BL via the analysis of a recombinant inbred line population bred from a cross between ‘YS116’ and ‘Sonalika.’ ‘YS116’ is an F12 selection from the cross ‘Yangmai 6’ (source of resistance) × ‘Sonalika,’ which contains only one spot blotch resistance QTL. Lines tested from F3 to F5 were inoculated in the field with B. sorokiniana over three consecutive seasons. Measurements of both disease severity and the area under the disease progress curve were used to assess each line’s disease response. In the present population, QSb.bhu-5B, which is flanked by the SSR loci Xgwm639 (0.28 cM) and Xgwm1043, behaved as a single Mendelian gene and was given the gene symbol Sb2. The physical location of Sb2 is within the 5BL1-0.55-0.75 deletion bin. Both Xgwm1043 and Xgwm639 were effective as indirect selection tools in various genetic backgrounds.

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Acknowledgments

All authors acknowledge the financial support from Department of Biotechnology, Government of India and the BMBF, Germany (Project 01DQ12016). Suneel Kumar was a beneficiary of a Department of Biotechnology JRF/SRF fellowship, granted under the Biotechnology Eligibility Test program. Banaras Hindu University is thanked for permitting the use of ‘YS116’ as the resistance donor. Dr. Raj Kumar Jat (BISA, Samastipur, India) is acknowledged for his experimental support and Anette Heber, Sonja Allner, Binod Kumar Mehato and Md. Aminul Islam for other technical assistance.

Author contributions

Suneel Kumar performed most of the experimental work as part of his Ph.D. thesis and drafted the manuscript. Marion S. Röder provided genotyping facility and facilitated genotyping of mapping population in IPK Gatersleben, Germany. Shashi Bhushan Tripathi extended the phenotyping facility at Gual Pahari and facilitated preliminary screening of parental lines and population with SSR markers in TERI, New Delhi. Sundeep Kumar performed statistical analysis, designed field layout for evaluation and helped in writing manuscript. Ramesh Chand provided spot blotch inoculum and participated in evaluation of lines for spot blotch resistance. Arun Kumar Joshi made scientific contribution for initiation and conduct of experiment and manuscript preparation. Uttam Kumar involved in over all experiment conduct and planning, analysis, result interpretation and helped in manuscript writing.

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11032_2015_411_MOESM1_ESM.docx

The effect on the AUDPC of the linked SSRs Xgwm1043 and Xgwm639. A: ‘YS116’ allele, B: ‘Sonalika’ allele in F3, F4 and F5 generation (DOCX 60 kb)

Supplementary material 2 (DOCX 27 kb)

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Kumar, S., Röder, M.S., Tripathi, S.B. et al. Mendelization and fine mapping of a bread wheat spot blotch disease resistance QTL. Mol Breeding 35, 218 (2015). https://doi.org/10.1007/s11032-015-0411-5

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