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A new powdery mildew resistance allele at the Pm4 wheat locus transferred from einkorn (Triticum monococcum)

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Abstract

Powdery mildew is one of the most destructive foliar diseases of wheat. A set of differential Blumeria graminis f.sp. tritici (Bgt) isolates was used to test the powdery mildew response of a Triticum monococcum-derived resistant hexaploid line, Tm27d2. Segregation analysis of 95 F2:3 lines from a Chinese Spring/Tm27d2 cross revealed that the resistance of Tm27d2 is controlled by a single dominant gene. Using monosomic analysis and a molecular mapping approach, the resistance gene was localized to the terminal end of chromosome 2AL. The linkage map of chromosome 2AL consisted of nine simple sequence repeat markers and one sequence-tagged site (STS) marker (ResPm4) indicative for the Pm4 locus. According to the differential reactions of 19 wheat cultivars/lines with known powdery mildew resistance genes to 13 Bgt isolates, Tm27d2 carried a new resistance specificity. The complete association of the resistance allele with STS marker ResPm4 indicated that it represented a new allele at the Pm4 locus. This new allele was designated Pm4d. The two flanking markers Xgwm526 and Xbarc122 closely linked to Pm4d at genetic distances of 3.4 and 1.0 cM, respectively, are present in chromosome bin 2AL1-0.85-1.00.

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Acknowledgments

We would like to thank A. Fiedler, J.A. Reiche and T. Wurmser for excellent technical assistance during the collection of the genotypic and phenotypic data.

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Authors and Affiliations

  1. Plant Breeding, Technische Universität München, Emil-Ramann-Str.4, 85354, Freising, Germany

    Michael Schmolke, Friedrich J. Zeller & Sai L. K. Hsam

  2. Institute for Crop Science and Plant Breeding, Bavarian State Research Center for Agriculture, Am Gereuth 8, 85354, Freising, Germany

    Volker Mohler & Lorenz Hartl

Authors
  1. Michael Schmolke
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  2. Volker Mohler
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  3. Lorenz Hartl
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  4. Friedrich J. Zeller
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  5. Sai L. K. Hsam
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Correspondence to Michael Schmolke.

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Schmolke, M., Mohler, V., Hartl, L. et al. A new powdery mildew resistance allele at the Pm4 wheat locus transferred from einkorn (Triticum monococcum). Mol Breeding 29, 449–456 (2012). https://doi.org/10.1007/s11032-011-9561-2

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  • DOI: https://doi.org/10.1007/s11032-011-9561-2

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