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Expression of Arabidopsis NDPK2 increases antioxidant enzyme activities and enhances tolerance to multiple environmental stresses in transgenic sweetpotato plants

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Abstract

Transgenic sweetpotato (Ipomoea batatas L. cv. Yulmi) plants expressing the Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) gene under the control of an oxidative stress–inducible peroxidase (SWPA2) promoter (referred to as SN plants) were developed and evaluated for enhanced tolerance of SN plants under various abiotic stress conditions. The level of AtNDPK2 expression and NDPK activity in SN plants following methyl viologen (MV) treatment was positively correlated with the plant’s tolerance to MV. Interestingly, we observed that antioxidant enzyme activities such as peroxidase, ascorbate peroxidase, and catalase increased in MV-treated SN plants. In addition, SN plants showed enhanced tolerance to cold, high salinity, and drought stresses by an increase in the activity of H2O2 scavenging enzymes. These results indicate that overexpression of AtNDPK2 in sweetpotato might efficiently modulate oxidative stress from various environmental stresses.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

Chl:

Chlorophyll

DAB:

3,3-Diaminobenzidine

MV:

Methyl viologen

NDPK:

Nucleoside diphosphate kinase

NT:

Non-transgenic

POD:

Peroxidase

ROS:

Reactive oxygen species

RWC:

Relative water content

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Acknowledgments

This work was supported by grants from the Biogreen21 Program (20070301034015) and the Bioenergy Program (20070201030040), Rural Development Administration, Korea, from the Korea Foundation for International Cooperation of Science and Technology (KICOS), Ministry of Education, Science and Technology (MEST), Korea, from the World Class University Program (R32-10148) supported by MEST, and KRIBB initiative program.

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Authors and Affiliations

  1. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Oun-dong 52, Yuseong-gu, Daejeon, 305-806, Korea

    Yun-Hee Kim, Soon Lim, Kyoung-Sil Yang, Cha Young Kim, Haeng-Soon Lee & Sang-Soo Kwak

  2. Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Korea

    Suk-Yoon Kwon

  3. Institute of Sweetpotato, Chinese Academy of Agricultural Sciences, Donghe East Suburb, 221121, Xuzhou, Jiangsu, China

    Xin Wang, Zhilin Zhou & Daifu Ma

  4. Division of Applied Life Science, and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Korea

    Dae-Jin Yun

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  1. Yun-Hee Kim
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Correspondence to Sang-Soo Kwak.

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Kim, YH., Lim, S., Yang, KS. et al. Expression of Arabidopsis NDPK2 increases antioxidant enzyme activities and enhances tolerance to multiple environmental stresses in transgenic sweetpotato plants. Mol Breeding 24, 233–244 (2009). https://doi.org/10.1007/s11032-009-9286-7

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  • DOI: https://doi.org/10.1007/s11032-009-9286-7

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