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The effects of quercetin protect cardiomyocytes from A/R injury is related to its capability to increasing expression and activity of PKCε protein

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Abstract

Quercetin is a ubiquitous flavonoid found in vegetable foods. Epidemiological and animal studies have reported an inverse association between quercetin intakes and occurrence and development of various cardiovascular diseases. Some researchers have inferred that the mechanisms of quercetin to protect cardiomyocytes from ischemia/reperfusion injury may be involved in modulation of intracellular signal pathways and regulation of proteins expression beyond its antioxidant activity. The aim of this study was to investigate whether quercetin protect cardiomyocytes from anoxia/reoxygenation injury through PKCε pathway. Neonatal rat primary cardiomyocytes were pretreated with quercetin or quercetin plus εV1-2, a selective PKCε inhibitor, prior to A/R treatment. Western blotting analysis showed that the level of PKCε and phosphor-PKCε Ser297 in the quercetin pretreatment group were all increased significantly compared to the control or A/R group. Subsequent assays showed that pretreated with quercetin could increase the viability of neonatal rat primary cardiomyocytes suffered A/R, decrease the apoptosis and ROS and alleviate the loss of mitochondrial membrane potential induced by A/R injury. However, the protective effects of quercetin disappeared in the group pretreated with εV1-2. Thus, for the first time, we revealed that one of the mechanisms of quercetin protecting cardiomyocytes from A/R injury might be increase the expression of PKCε protein and then enhance the activity of its downstream pathway.

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Acknowledgments

This work was supported by 973 projects (2009CB526405) to H.M. and Natural Science Foundation of China (81260492) to T.L.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, People’s Republic of China

    Lei Tang, Yian Peng & Ming He

  2. Department of Pharmacology & Molecular Therapeutics, Nanchang University School of Pharmaceutical Science, Nanchang, 330006, People’s Republic of China

    Lei Tang, Yian Peng, Tiantian Xu, Xiaoqing Yi, Ying Liu, Yuchao Luo & Ming He

  3. Jiangxi Provincial Key Laboratory of Molecular Medicine at the Second Affiliated Hospital, Nanchang University, Nanchang, 330006, People’s Republic of China

    Dong Yin

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  1. Lei Tang
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  2. Yian Peng
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  3. Tiantian Xu
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  4. Xiaoqing Yi
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  5. Ying Liu
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  7. Dong Yin
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  8. Ming He
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Correspondence to Ming He.

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Lei Tang and Yian Peng have contributed equally to this study.

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Tang, L., Peng, Y., Xu, T. et al. The effects of quercetin protect cardiomyocytes from A/R injury is related to its capability to increasing expression and activity of PKCε protein. Mol Cell Biochem 382, 145–152 (2013). https://doi.org/10.1007/s11010-013-1729-0

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  • DOI: https://doi.org/10.1007/s11010-013-1729-0

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