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Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats

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Abstract

Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight). The rats were weighed weekly and sacrificed after 6 months. The glandular portion of the stomach was removed and processed for H+-K+-ATPase immunohistochemistry and light and electron microscopy studies. Acid secretion was measured in vivo. After 6 months of diabetes, the mean weight of the rats was significantly lower (P < 0.001) compared to control. The mean weight of the stomach to body weight percentage increased significantly (P < 0.001) compared to control. The blood glucose level in diabetic rats was significantly higher (P < 0.001) than in normal control. Diabetic rats showed significant (P < 0.001) decrease in basal and stimulated acid secretion when compared to control. Electron micrographs of the parietal cells of glandular stomach of diabetic rats revealed significant (P < 0.0002) reduction in the number of mitochondria and a small though not significant increase in the number of canaliculi in the parietal cells compared with normal. Immunohistochemistry showed reduced H+-K+-ATPase (P < 0.00001) compared to control. Long-term diabetes induces morphological as well as functional changes in gastric parietal cells. The decrease in the number of mitochondria accompanied by reduced in H+-K+-ATPase in parietal cells may explain the reduced acid secretion observed in diabetics.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, UAE

    Salim M. A. Bastaki, Irwin S. Chandranath, Naheed Amir & Abdu Adem

  2. Department of Anatomy, Faculty of Medicine and Health Sciences, UAE University, P.O. Box 17666, Al Ain, UAE

    Ernest Adeghate, Saeed Tariq & Rashed S. Hameed

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  1. Salim M. A. Bastaki
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  2. Ernest Adeghate
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Correspondence to Salim M. A. Bastaki.

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Bastaki, S.M.A., Adeghate, E., Chandranath, I.S. et al. Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats. Mol Cell Biochem 341, 43–50 (2010). https://doi.org/10.1007/s11010-010-0435-4

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  • DOI: https://doi.org/10.1007/s11010-010-0435-4

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