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Isolation and Identification of Three Novel Antioxidant Peptides from the Bactrian Camel Milk Hydrolysates

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Abstract

The aim of this study was isolation and purification of antioxidant peptides from Bactrian camel milk (BCM) hydrolysate. Trypsin, pepsin, alcalase, and papain were used for hydrolysis of BCM. The peptides fraction from trypsin digest exhibited a strong antioxidant activity among them, and it was separated by ultrafiltration (3000 Da membrane). The resulting peptides (< 3000 Da) were further separated with using gel filtration chromatography (HW-55F) and reverse-phase high-performance liquid chromatography (RP-HPLC). Three novel antioxidant peptides were purified and sequenced by MALDI TOF–MS/MS as RLDGQGRPRVWLGR (TFI-b1), TPDNIDIWLGGIAEPQVKR (TFI-b2) and VAYSDDGENWTEYRDQGAVEGK (TFI-b3) with molecular weights 1665.94, 2122.13, and 2489.09 Da, respectively. The IC50 values of these peptides on DPPH were 1.9, 1.2 and 0.6 mg/mL and on ABTS are 2.4, 1.8 and 0.9 mg/mL, respectively. The peptides identified using similarity search tool of SIB ExPASy Bioinformatics Resources Portal (http://www.expasy.org).

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Acknowledgements

This study was supported by Major Special Project of National Science and Technology, Research and Development of New ethnic Drug varieties and its key Innovation Technology (2017ZX09301045) and thank the Central Asia Drug Research and Development Center of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing South Road 40-1, Ürümqi, 830011, Xinjiang, People’s Republic of China

    Ahmidin Wali, Gao Yanhua, Abulimiti Yili & Haji Akber Aisa

  2. A. S. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, 100125, Uzbekistan

    Uchkun Ishimov & Shavkat Salikhov

  3. University of Chinese Academy of Science, Beijing, 100039, People’s Republic of China

    Ahmidin Wali

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  1. Ahmidin Wali
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  2. Gao Yanhua
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  3. Uchkun Ishimov
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  4. Abulimiti Yili
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  5. Haji Akber Aisa
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Correspondence to Abulimiti Yili.

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Wali, A., Yanhua, G., Ishimov, U. et al. Isolation and Identification of Three Novel Antioxidant Peptides from the Bactrian Camel Milk Hydrolysates. Int J Pept Res Ther 26, 641–650 (2020). https://doi.org/10.1007/s10989-019-09871-x

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